Anti-STEAP1 抗体 (ab3679)

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Western blot - Anti-STEAP1 antibody (ab3679)

    Key features and details

    • Rabbit polyclonal to STEAP1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-STEAP1 antibody
      STEAP1 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to STEAP1

    • 由来種

      Rabbit

    • 特異性

      This antibody reacts predominantly with STEAP. Ab3679 can be used as a plasma marker for prostate cancer cells

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human

    • 免疫原

      Synthetic peptide corresponding to Human STEAP1 aa 14-28 conjugated to keyhole limpet haemocyanin.
      Sequence:

      WKMKPRRNLEEDDYL

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • ポジティブ･コントロール

      • WB: A431 cell lysate

    • 特記事項


      Prostate cancer is the most frequently diagnosed cancer and is the second leading cause of cancer-related deaths in American men. Although the early serum detection of prostate-specific antigen (PSA) plays important roles in early diagnosis, it may not distinguish prostate cancer from benign diseases. Recently, a novel protein, which contains 339 amino acids, named STEAP (six transmembrane epithelial antigen of the prostate), was identified in advanced prostate cancer. STEAP is unique among the currently known prostate cancer markers because of its putative secondary structure, from which one may predict that it functions as a potential channel protein. STEAP is strongly expressed in advanced prostate cancer and some extent expression in other cancers, such as colon and ovarian cancer cell lines.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • バッファー

      pH: 7.40
      Preservative: 0.05% Sodium azide
      Constituents: PBS, 0.1% BSA
    • Concentration information loading...

    • 精製度

      Immunogen affinity purified

    • 一次抗体 備考

      Prostate cancer is the most frequently diagnosed cancer and is the second leading cause of cancer-related deaths in American men. Although the early serum detection of prostate-specific antigen (PSA) plays important roles in early diagnosis, it may not distinguish prostate cancer from benign diseases. Recently, a novel protein, which contains 339 amino acids, named STEAP (six transmembrane epithelial antigen of the prostate), was identified in advanced prostate cancer. STEAP is unique among the currently known prostate cancer markers because of its putative secondary structure, from which one may predict that it functions as a potential channel protein. STEAP is strongly expressed in advanced prostate cancer and some extent expression in other cancers, such as colon and ovarian cancer cell lines.

    • ポリ/モノ

      ポリクローナル

    • アイソタイプ

      IgG

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab3679の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa).
    特記事項
    WB
    Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa).

    ターゲット情報

    • 機能

      Metalloreductase that has the ability to reduce both Fe(3+) to Fe(2+) and Cu(2+) to Cu(1+). Uses NAD(+) as acceptor.

    • 組織特異性

      Ubiquitously expressed. Highly expressed in prostate tumors.

    • 配列類似性

      Belongs to the STEAP family.
      Contains 1 ferric oxidoreductase domain.

    • 細胞内局在

      Endosome membrane.
    • Information by UniProt

    • 参照データベース

      • 別名

        • 2410007B19Rik antibody
        • EC 1.16.1.- antibody
        • Metalloreductase STEAP1 antibody
        • MGC19484 antibody
        • PRSS 24 antibody
        • PRSS24 antibody
        • Six transmembrane epithelial antigen of prostate antibody
        • Six transmembrane epithelial antigen of the prostate 1 antibody
        • Six transmembrane epithelial antigen of the prostate antibody
        • Six-transmembrane epithelial antigen of prostate 1 antibody
        • STEA1_HUMAN antibody
        • STEAP 1 antibody
        • Steap antibody
        • STEAP1 antibody
        see all

      画像

      • Western blot - Anti-STEAP1 antibody (ab3679)
        Western blot - Anti-STEAP1 antibody (ab3679)
        Anti-STEAP1 antibody (ab3679) at 2 µg/ml + A431 cell lysate

        Predicted band size: 38 kDa

      参考文献 (1)

      ab3679 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab3679 は 1 報の論文で使用されています。

      • Jiang JN  et al. EIF4E regulates STEAP1 expression in peritoneal metastasis. J Cancer 11:990-996 (2020). PubMed: 31949502

      レビューと Q&A

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      Question

      Sorry for the delay, however I just heard back from the customer after several contact attempts. I have attached their answers to your questions. In answer to your questions: 1) We see many non specific bands (please see picture attached for a sample) 2) Antibody is being tested on EL-4 (mouse T cells) that have been transfected with pcDNA3 encoded with human STEAP. WB was done on whole cell extract. 3) Sample were lysed with SDS, protease inhibitors, b-mercapto, EDTA, etc. Samples were heated for 10 min at 100'C. 4) Ab was raised against Human STEAP. Dilutions used were 1/500, 1/1000, 1/2000, 1/6000. Incubation time of 1hour followed by three thorough washing steps. 5) Secondary antibody is an anti-rabbit. Incubated for 45 min followed by three thorough washing steps. Secondary antibody is commonly used by numerous members of our lab with no problems. 6) ECL kit. 7) Secondary antibody has been run alone (with no primary antibody) and shows no background whatsoever. Blocking and washing steps are sufficient and stringent. We block o/n with agitation at 4 degrees. Washing is has been done 3 times for 10 min each, or 6 times for 5 min each. Bands cannot be degraded products as they are at a higher molecular weight then the full-length expected Band size. 8) Westerns were done many times with the same results. Altered steps were washing times, antibody concentration, altered incubation times. 9) Negative controls showed the same result to a lesser intensity.no positive controls were used. Just to recap, this is the customer that found the same result with his transfected and non-transfected cells (control) and saw no difference in staining. He does not get the 40kDa band that he expects to see (I will ask to see if the customer can show the MWts of the control bands), but a lot of other bands instead. Customer is very unhappy and wants a credit. I look forward to your response,

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      Answer

      Thank you for your enquiry and your patience. We are very sorry that the customer is having problem with this product. We have read through the forwarded protocol and it seems to be fine. After careful consideration, we offer a credit note. We apologize for any inconvenience caused and thank you for bringing this matter to our attention.

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