製品の概要

  • 製品名

    Anti-Stathmin 1 antibody [EP1573Y]
    Stathmin 1 一次抗体 製品一覧
  • 製品の詳細

    Rabbit monoclonal [EP1573Y] to Stathmin 1
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: ICC/IF, WB, IP, Flow Cyt, IHC-Pmore details
  • 種交差性

    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human Stathmin 1 aa 100 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P16949

  • ポジティブ・コントロール

    • WB: Human lymph node, Mouse brain, PC-12 whole cell lysates IP: human colon and HeLa cells. IHC-P: lymph node, Human lung carcinoma, Mouse brain, and Rat brain tissue. FC: Jurkat cells ICC/IF: HeLa cells
  • 特記事項

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/100 - 1/250.
WB 1/10000. Detects a band of approximately 19 kDa.

For unpurified use at 1/50000

IP 1/30.

For unpurified use at 1/50

Flow Cyt 1/60.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

For unpurified use at 1/100

IHC-P 1/2000.

See IHC antigen retrieval protocols

ターゲット情報

  • 機能

    Involved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear.
  • 組織特異性

    Ubiquitous. Expression is strongest in fetal and adult brain, spinal cord, and cerebellum, followed by thymus, bone marrow, testis, and fetal liver. Expression is intermediate in colon, ovary, placenta, uterus, and trachea, and is readily detected at substantially lower levels in all other tissues examined. Lowest expression is found in adult liver. Present in much greater abundance in cells from patients with acute leukemia of different subtypes than in normal peripheral blood lymphocytes, non-leukemic proliferating lymphoid cells, bone marrow cells, or cells from patients with chronic lymphoid or myeloid leukemia.
  • 配列類似性

    Belongs to the stathmin family.
    Contains 1 SLD (stathmin-like) domain.
  • 翻訳後修飾

    Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. MAPK is responsible for the phosphorylation of stathmin in response to NGF. Phosphorylation at Ser-16 seems to be required for neuron polarization (By similarity). Phosphorylation at Ser-63 reduces tubulin binding 10-fold and suppresses the MT polymerization inhibition activity.
  • 細胞内局在

    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 参照データベース

  • 別名

    • C1orf215 antibody
    • Lag antibody
    • LAP 18 antibody
    • LAP18 antibody
    • Leukemia associated phosphoprotein p18 antibody
    • Leukemia-associated phosphoprotein p18 antibody
    • Metablastin antibody
    • Oncoprotein 18 antibody
    • OP 18 antibody
    • Op18 antibody
    • p18 antibody
    • p19 antibody
    • Phosphoprotein 19 antibody
    • Phosphoprotein p19 antibody
    • pp17 antibody
    • pp19 antibody
    • PR22 antibody
    • Pr22 protein antibody
    • Prosolin antibody
    • Protein Pr22 antibody
    • SMN antibody
    • Stathmin antibody
    • Stathmin1 antibody
    • STMN 1 antibody
    • Stmn1 antibody
    • STMN1_HUMAN antibody
    see all

画像

  • Anti-Stathmin 1 antibody [EP1573Y] (ab52630) at 1/50000 dilution (Purified) + Mouse brain lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Observed band size: 18 kDa
    why is the actual band size different from the predicted?

  • Anti-Stathmin 1 antibody [EP1573Y] (ab52630) at 1/10000 dilution (Purified) + Human lymph node lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 18 kDa why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Stathmin 1 with purified ab52630 at 1/200 dilution (3.1 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 µg/ml) dilution. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Anti-Stathmin 1 antibody [EP1573Y] (ab52630) at 1/500000 dilution + PC12 lysate at 10 µg

    Secondary
    goat anti-rabbit HRP at 1/2000 dilution

    Observed band size: 18 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling Stathmin 1 with purified ab52630 at 1/2000 dilution (0.31 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • ab52630 (purified) at 1/30 dilution (2ug) immunoprecipitating Stathmin 1 in HeLa whole cell lysates.
    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates 10ug
    Lane 2 (+): ab52630 & HeLa whole cell lysates
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52630 in HeLa whole cell lysates
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling Stathmin 1 with purified ab52630 at 1/60 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat brain tissue sections labeling Stathmin 1 with purified ab52630 at 1/2000 dilution (0.31 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse brain tissue sections labeling Stathmin 1 with purified ab52630 at 1/2000 dilution (0.31 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • ab52630 stained HeLa cells
  • ab52630, at a 1/250 dilution, staining human Stathmin 1 in lymph node tissue, using Immunohistochemistry, Paraffin embedded sections.
  • Overlay histogram showing Jurkat cells stained with ab52630 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52630, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) ( 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • Whole cell lysate prepared from human colon cells was loaded at 20µg. The immunoprecipitation step was performed using Protein A/G. ab52630 used at a 1/200 dilution for 12 hours at 4°C. For WB ab52630 used at a 1/10000 dilution.

    See Abreview

参考文献

This product has been referenced in:

  • Ke B  et al. Clinical significance of Stathmin1 expression and epithelial-mesenchymal transition in curatively resected gastric cancer. Mol Clin Oncol 10:214-222 (2019). Read more (PubMed: 30680197) »
  • Yan L  et al. A novel rapid quantitative method reveals stathmin-1 as a promising marker for esophageal squamous cell carcinoma. Cancer Med 7:1802-1813 (2018). Read more (PubMed: 29577639) »
See all 19 Publications for this product

レビューと Q&A

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1-5 of 5 Abreviews

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Adult skin)
Permeabilization
No
Specification
Adult skin
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Fixative
unfixed

Mr. Julien Coutier

Verified customer

投稿 Jul 02 2018

Application
Western blot
Sample
Human Cell lysate - whole cell (primary keratinocytes)
Gel Running Conditions
Reduced Denaturing (any kD BioRad)
Loading amount
50 µg
Specification
primary keratinocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Mr. Julien Coutier

Verified customer

投稿 Jul 02 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain, Dentate gyrus)
Permeabilization
Yes - TritonX-100(0.05%)
Specification
Brain, Dentate gyrus
Fixative
Formaldehyde

Abcam user community

Verified customer

投稿 Mar 27 2018

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (Colon)
Total protein in input
20 µg
Specification
Colon
Immuno-precipitation step
Protein A/G

Mr. Qifeng Lin

Verified customer

投稿 Jul 06 2011

Application
Western blot
Sample
Human Cell lysate - whole cell (HCT-116)
Loading amount
20 µg
Specification
HCT-116
Gel Running Conditions
Non-reduced Denaturing (12.5)
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 23°C

Mr. Qifeng Lin

Verified customer

投稿 Jan 13 2011

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