Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Non-Denaturing (Native) (5-16% gel)
Sample
Human Cell lysate - whole cell (Jurkat and Hela cells)
Specification
Jurkat and Hela cells
Treatment
see below
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer (5%BSA in TBST)
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit IgG HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
chemiluminescence
Bands
Specific: 90 kDa
Exposure
5 minute(s) and 0 second(s)
Negative control
-
Positive control
A-431 cell lysate
Additional data
Additional Notes
bands:
1 = Hela cells (untreated) pStat5
2 = Hela cells (IFNa treated) pStat5
3 = Jurkat cells (IFNa treated) pStat5
4 = Jurkat cells (untreated) pStat5
5 = positive control (A-431 cell lysate)
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投稿 Mar 17 2015