Anti-STAT1 (phospho S727) 抗体 (ab4742)

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Key features and details

  • Rabbit polyclonal to STAT1 (phospho S727)
  • Suitable for: WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-STAT1 (phospho S727) antibody
    STAT1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to STAT1 (phospho S727)

  • 由来種

    Rabbit

  • 特異性

    This antibody does not react with non-phosphorylated STAT 1 protein.

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Mouse, Human

  • 免疫原

    Synthetic peptide (Human) derived from the region of human STAT 1 that contains serine 727. This region is conserved between human and mouse.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab4742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 91 kDa (predicted molecular weight: 91 kDa).

ターゲット情報

  • 機能

    Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state.

  • 関連疾患

    Note=STAT1 deficiency results in impaired immune response leading to severe mycobacterial and viral diseases. In the case of complete deficiency, patients can die of viral disease.
    Defects in STAT1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance.

  • 配列類似性

    Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.

  • 翻訳後修飾

    Phosphorylated on tyrosine and serine residues in response to IFN-alpha, IFN-gamma, PDGF and EGF. Phosphorylation on Tyr-701 (lacking in beta form) by JAK promotes dimerization and subsequent translocation to the nucleus. Phosphorylation on Ser-727 by several kinases including MAPK14, ERK1/2 and CAMKII on IFN-gamma stimulation, regulates STAT1 transcriptional activity. Phosphorylation on Ser-727 promotes sumoylation though increasing interaction with PIAS. Phosphorylation on Ser-727 by PKCdelta induces apoptosis in response to DNA-damaging agents.
    Sumoylated by SUMO1, SUMO2 and SUMO3. Sumoylation is enhanced by IFN-gamma-induced phosphorylation on Ser-727, and by interaction with PIAS proteins. Enhances the transactivation activity.
    ISGylated.

  • 細胞内局在

    Cytoplasm. Nucleus. Translocated into the nucleus in response to IFN-gamma-induced tyrosine phosphorylation and dimerization.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Signal transducer and activator of transcription 1 91kD antibody
      • CANDF7 antibody
      • DKFZp686B04100 antibody
      • IMD31A antibody
      • IMD31B antibody
      • IMD31C antibody
      • ISGF 3 antibody
      • ISGF-3 antibody
      • OTTHUMP00000163552 antibody
      • OTTHUMP00000165046 antibody
      • OTTHUMP00000165047 antibody
      • OTTHUMP00000205845 antibody
      • Signal transducer and activator of transcription 1 91kDa antibody
      • Signal transducer and activator of transcription 1 antibody
      • Signal transducer and activator of transcription 1, 91kD antibody
      • Signal transducer and activator of transcription 1-alpha/beta antibody
      • STAT 1 antibody
      • Stat1 antibody
      • STAT1_HUMAN antibody
      • STAT91 antibody
      • Transcription factor ISGF 3 components p91 p84 antibody
      • Transcription factor ISGF-3 components p91/p84 antibody
      • Transcription factor ISGF3 components p91/p84 antibody
      • XStat1 antibody
      see all

    画像

    • Western blot - Anti-STAT1 (phospho S727) antibody (ab4742)
      Western blot - Anti-STAT1 (phospho S727) antibody (ab4742)

      To demonstrate the phosphorylation of STAT 1 [pS727], 3T3- L1 cells were treated with 50 nM insulin for 15 minutes, followed by treatment with 100 units/mL IFN-gamma for 15 minutes. Proteins from serum-starved (Lane 1) and serumstarved/ insulin and INF gamma treated (Lane 2) 3T3-L1 cell extracts were resolved by SDS-PAGE on a 4-20% Trisglycine gel. The proteins were then transferred to nitrocellulose. Membranes were incubated with 1 µg/mL anti-STAT 1 [pS727]. After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar detection method. The Western blotting data indicate that STAT 1 is phosphorylated at serine 727 upon exposure to insulin followed by IFN-gamma.

       

      To demonstrate the phosphorylation of STAT 1 [pS727], 3T3- L1 cells were treated with 50 nM insulin for 15 minutes, follow

    参考文献 (7)

    ab4742 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab4742 は 7 報の論文で使用されています。

    • Rychtarcikova Z  et al. Tumor-initiating cells of breast and prostate origin show alterations in the expression of genes related to iron metabolism. Oncotarget 8:6376-6398 (2017). WB ; Human . PubMed: 28031527
    • Feng J  et al. Dnmt1 and Dnmt3a maintain DNA methylation and regulate synaptic function in adult forebrain neurons. Nat Neurosci 13:423-30 (2010). IHC ; Mouse . PubMed: 20228804
    • Modestou MA  et al. Inhibition of IFN-gamma-dependent antiviral airway epithelial defense by cigarette smoke. Respir Res 11:64 (2010). WB ; Human . PubMed: 20504369
    • Mastronardi FG  et al. Synergy between paclitaxel plus an exogenous methyl donor in the suppression of murine demyelinating diseases. Mult Scler 13:596-609 (2007). PubMed: 17548438
    • Tjandra SS  et al. IFN-{beta} signaling positively regulates tumorigenesis in aggressive fibromatosis, potentially by modulating mesenchymal progenitors. Cancer Res 67:7124-31 (2007). PubMed: 17671179
    • Rosner D  et al. Interferon-gamma induces Fas trafficking and sensitization to apoptosis in vascular smooth muscle cells via a PI3K- and Akt-dependent mechanism. Am J Pathol 168:2054-63 (2006). PubMed: 16723718
    • Tropea D  et al. Gene expression changes and molecular pathways mediating activity-dependent plasticity in visual cortex. Nat Neurosci 9:660-8 (2006). PubMed: 16633343

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    Western blot abreview for Anti-STAT1 (phospho S727) antibody

     Average
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human umbilical vein endothelial cells)
    Loading amount
    50 µg
    Specification
    Human umbilical vein endothelial cells
    Treatment
    Timecourse of IFN-gamma (0, 4, 8, 24 hours)
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
    Read More

    Mr. Saydul Alam

    Verified customer

    投稿 Apr 19 2011

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