Anti-STAT1 抗体 [SM1] (ab3987)

Reviews (3)Q&A (1)References (17)
Western blot - Anti-STAT1 antibody [SM1] (ab3987)
  • Flow Cytometry - Anti-STAT1 antibody [SM1] (ab3987)

Key features and details

  • Mouse monoclonal [SM1] to STAT1
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Human
  • Isotype: IgG2b

製品の概要

  • 製品名

    Anti-STAT1 antibody [SM1]
    STAT1 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [SM1] to STAT1

  • 由来種

    Mouse

  • 特異性

    The antibody recognizes an epitope included within amino acids 721-733 of the 91 kDa STAT 1 protein.

  • アプリケーション

    適用あり: WB, Flow Cytmore details

  • 種交差性

    交差種: Mouse, Human

  • 免疫原

    Synthetic peptide:

    DNLLPMSPEEFDE

    , corresponding to amino acids 721-733 of STAT 1.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab3987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Detects a band of approximately 91 kDa (predicted molecular weight: 87 kDa).
Flow Cyt Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能

    Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state.

  • 関連疾患

    Note=STAT1 deficiency results in impaired immune response leading to severe mycobacterial and viral diseases. In the case of complete deficiency, patients can die of viral disease.
    Defects in STAT1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance.

  • 配列類似性

    Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.

  • 翻訳後修飾

    Phosphorylated on tyrosine and serine residues in response to IFN-alpha, IFN-gamma, PDGF and EGF. Phosphorylation on Tyr-701 (lacking in beta form) by JAK promotes dimerization and subsequent translocation to the nucleus. Phosphorylation on Ser-727 by several kinases including MAPK14, ERK1/2 and CAMKII on IFN-gamma stimulation, regulates STAT1 transcriptional activity. Phosphorylation on Ser-727 promotes sumoylation though increasing interaction with PIAS. Phosphorylation on Ser-727 by PKCdelta induces apoptosis in response to DNA-damaging agents.
    Sumoylated by SUMO1, SUMO2 and SUMO3. Sumoylation is enhanced by IFN-gamma-induced phosphorylation on Ser-727, and by interaction with PIAS proteins. Enhances the transactivation activity.
    ISGylated.

  • 細胞内局在

    Cytoplasm. Nucleus. Translocated into the nucleus in response to IFN-gamma-induced tyrosine phosphorylation and dimerization.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Signal transducer and activator of transcription 1 91kD antibody
      • CANDF7 antibody
      • DKFZp686B04100 antibody
      • IMD31A antibody
      • IMD31B antibody
      • IMD31C antibody
      • ISGF 3 antibody
      • ISGF-3 antibody
      • OTTHUMP00000163552 antibody
      • OTTHUMP00000165046 antibody
      • OTTHUMP00000165047 antibody
      • OTTHUMP00000205845 antibody
      • Signal transducer and activator of transcription 1 91kDa antibody
      • Signal transducer and activator of transcription 1 antibody
      • Signal transducer and activator of transcription 1, 91kD antibody
      • Signal transducer and activator of transcription 1-alpha/beta antibody
      • STAT 1 antibody
      • Stat1 antibody
      • STAT1_HUMAN antibody
      • STAT91 antibody
      • Transcription factor ISGF 3 components p91 p84 antibody
      • Transcription factor ISGF-3 components p91/p84 antibody
      • Transcription factor ISGF3 components p91/p84 antibody
      • XStat1 antibody
      see all

    画像

    • Western blot - Anti-STAT1 antibody [SM1] (ab3987)
      Western blot - Anti-STAT1 antibody [SM1] (ab3987)

      Induction of phosphorylation of STAT 1 at Ser727 in human malignant melanoma cells (short-term culture derived from a patient) in response to interferons. Subconfluent cells were serum-starved before exposure to activation dosages of IFN-gamma (10 ng/ml) and IFN-alpha (1000 IU/ml and 5000 IU/ml). Western blotting analysis of cell extracts shows detection of phosphorylated STAT-1 (Ser727) by the antibody PSM1 (upper panel) and total STAT 1 level by the antibody SM1 (lower panel).
      Induction of phosphorylation of STAT 1 at Ser727 in human malignant melanoma cells (short-term culture derived from a patient) in response to interferons. Subconfluent cells were serum-starved before exposure to activation dosages of IFN-gamma (10 ng/ml) and IFN-alpha (1000 IU/ml and 5000 IU/ml). Western blotting analysis of cell extracts shows detection of phosphorylated STAT-1 (Ser727)
    • Flow Cytometry - Anti-STAT1 antibody [SM1] (ab3987)
      Flow Cytometry - Anti-STAT1 antibody [SM1] (ab3987)
      Overlay histogram showing HeLa cells stained with ab3987 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab3987, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    参考文献 (17)

    ab3987 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab3987 は 17 報の論文で使用されています。

    • Zhou J  et al. LncGBP9/miR-34a axis drives macrophages toward a phenotype conducive for spinal cord injury repair via STAT1/STAT6 and SOCS3. J Neuroinflammation 17:134 (2020). PubMed: 32345320
    • Chen FX  et al. Inflammation-dependent downregulation of miR-532-3p mediates apoptotic signaling in human sarcopenia through targeting BAK1. Int J Biol Sci 16:1481-1494 (2020). PubMed: 32226296
    • Wang Y  et al. A lipid-soluble extract of Pinellia pedatisecta Schott enhances antitumor T cell responses by restoring tumor-associated dendritic cell activation and maturation. J Ethnopharmacol 241:111980 (2019). PubMed: 31146000
    • Li X  et al. Silence of MEG3 intensifies lipopolysaccharide-stimulated damage of human lung cells through modulating miR-4262. Artif Cells Nanomed Biotechnol 47:2369-2378 (2019). PubMed: 31184231
    • Shi Q  et al. microRNA-29a functions as a tumor suppressor in nasopharyngeal carcinoma 5-8F cells through targeting VEGF. Iran J Basic Med Sci 22:541-546 (2019). PubMed: 31217935
    View all Publications for this product

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    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-STAT1 antibody [SM1]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - whole cell (macrophage)
    Specification
    macrophage
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 23 2014

    Western blot abreview for Anti-STAT1 antibody [SM1]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    2e+006 cells
    Gel Running Conditions
    Reduced Denaturing (10%)
    Sample
    Human Cell lysate - whole cell (primary human monocyte derived macrophages)
    Specification
    primary human monocyte derived macrophages
    Treatment
    100ug/ml MDP
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 26 2013

    Flow Cytometry abreview for Anti-STAT1 antibody [SM1]

     Poor
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Fixation
    BD #554722
    Sample
    Human Cell (primary human macrophages)
    Specification
    primary human macrophages
    Gating Strategy
    macrophages
    Permeabilization
    Yes - BD Phosflow #558050
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 15 2013

    Question

    Dr. Hayward: Thank you for update the abcam antibody. I would like to try your STAT1 (ab3987), some reason the order on line is so slow, it is hard to go through. Would you please help me to order it by other way?

    Read More
    Answer

    If you find the online order system too slow you can e-mail your order to orders@abcam.com or phone on 01223 472030 (dialing code +44) or fax your order on 01223 472038. Hope this helps.

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