Application
Western blot
Sample
Human Cell lysate - whole cell (THP-1)
Loading amount
25 µg
Specification
THP-1
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST + 2.5% milk
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit IgG
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL
Exposure
1 minute(s) and 30 second(s)
Bands
Specific: 30-32 kDa
Additional data
Additional Notes
The StarD1 band at 30-32 kDa was easily detected. Also another band below the 43 kDa marker showed up, and that could be the full length StarD1 (37 kDa). Some non-specific bands showed up at the top of the membrane.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Sep 29 2010