Anti-StAR 抗体 (ab96637)
Key features and details
- Rabbit polyclonal to StAR
- Suitable for: ICC, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-StAR antibody
StAR 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to StAR -
由来種
Rabbit -
アプリケーション
適用あり: ICC, WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Cow, Dog, Pig -
免疫原
Recombinant protein fragment corresponding to a region within amino acids 15 - 238 of Human StAR (NP_000340).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96637の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC |
Use at an assay dependent concentration.
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WB | (3) |
1/100 - 1/10000. Predicted molecular weight: 32 kDa.
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IHC-P |
1/100 - 1/1000.
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特記事項 |
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ICC
Use at an assay dependent concentration. |
WB
1/100 - 1/10000. Predicted molecular weight: 32 kDa. |
IHC-P
1/100 - 1/1000. |
ターゲット情報
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機能
Plays a key role in steroid hormone synthesis by enhancing the metabolism of cholesterol into pregnenolone. Mediates the transfer of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane where it is cleaved to pregnenolone. -
組織特異性
Expressed in gonads, adrenal cortex and kidney. -
パスウェイ
Steroid metabolism; cholesterol metabolism. -
関連疾患
Defects in STAR are the cause of adrenal hyperplasia type 1 (AH1) [MIM:201710]. The most severe form of adrenal hyperplasia. It is a condition characterized by onset of profound adrenocortical insufficiency shortly after birth, hyperpigmentation reflecting increased production of pro-opiomelanocortin, elevated plasma renin activity as a consequence of reduced aldosterone synthesis, and male pseudohermaphroditism resulting from deficient fetal testicular testosterone synthesis. Affected individuals are phenotypic females irrespective of gonadal sex, and frequently die in infancy if mineralocorticoid and glucocorticoid replacement are not instituted. -
配列類似性
Contains 1 START domain. -
細胞内局在
Mitochondrion. - Information by UniProt
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参照データベース
- Entrez Gene: 6770 Human
- Omim: 600617 Human
- SwissProt: P49675 Human
- SwissProt: Q28996 Pig
- Unigene: 521535 Human
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別名
- Cholesterol trafficker antibody
- Luteinizing hormone induced protein antibody
- mitochondrial antibody
see all
画像
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Anti-StAR antibody (ab96637) at 1/1000 dilution + K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 32 kDa12% SDS-PAGE
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Immunohistochemical analysis of paraffin-embedded human hepatoma tissue staining StAR protein at cytosol with ab96637 at 1/500.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min. -
Immunofluorescence analysis of 4% paraformaldehyde-fixed HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate labeling StAR protein at mitochondria with ab96637 at 1/500 dilution. Blue: Hoechst 33342 staining.
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All lanes : Anti-StAR antibody (ab96637) at 1/5000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : Transfected HEK-293T whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 32 kDa12% SDS-PAGE
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All lanes : Anti-StAR antibody (ab96637) at 1/1000 dilution
Lane 1 : Whole tissue lysate prepared from murine adrenal gland
Lane 2 : Whole tissue lysate prepared from rat adrenal gland
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-rabbit polyclonal at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 32 kDa
Exposure time: 20 seconds
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (21)
ab96637 は 21 報の論文で使用されています。
- Manna PR et al. Expression and Function of StAR in Cancerous and Non-Cancerous Human and Mouse Breast Tissues: New Insights into Diagnosis and Treatment of Hormone-Sensitive Breast Cancer. Int J Mol Sci 24:N/A (2023). PubMed: 36614200
- Mohr MA et al. Puberty enables oestradiol-induced progesterone synthesis in female mouse hypothalamic astrocytes. J Neuroendocrinol 34:e13082 (2022). PubMed: 35000221
- Gudelska M et al. Chemerin Affects P4 and E2 Synthesis in the Porcine Endometrium during Early Pregnancy. Int J Mol Sci 23:N/A (2022). PubMed: 35055130
- Przygrodzka E et al. PKA and AMPK Signaling Pathways Differentially Regulate Luteal Steroidogenesis. Endocrinology 162:N/A (2021). PubMed: 33502468
- Plewes MR et al. Trafficking of cholesterol from lipid droplets to mitochondria in bovine luteal cells: Acute control of progesterone synthesis. FASEB J 34:10731-10750 (2020). PubMed: 32614098