Anti-Staphylococcus aureus 抗体 (ab20920)

The Hamza and Li publication BMC Microbiol 14:207 (2014) cites ab20920 as detecting the ATCC 25923 strain so there is evidence it will cross react.

The cells used for the immunogen started out as whole cells that were disrupted by sonication and freeze/thaw cycles. Therefore the immunogen is a mixture of intact Staph aureus organisms and soluble proteins from disrupted cells. This is no record of UV-inactivation.

Thank you very much for your email.
There is absolutely no problem, the antibody will still work properly in my opinion.
Indeed, we have tested different antibodies, and even after a week at 37 degrees Celcius, the antibodies did not seem to loose activity. I am therefore confident that the antibody ab20920 you received, will still work perfectly fine.
If against expectations the antibody would not work, do please contact us again and we will send a replacement vial.
I hope this information is helpful. Please do not hesitate to contact us again, should you have any further question.

Thank you for contacting us.

Please find below the information I managed to collect by contacting the sources of the Anti-Staphylococcus aureusand the Anti-Streptococcus agalactiaeantibodies we have in our catalog :

Anti-Staphylococcus aureus:
https://www.abcam.com/ab23568 shows no reactivity to any streptococcus sp., mycoplasma sp. or corynebacteria sp.

Unfortunately, ab35192 has not been specifically tested against streptococcus so we are unable to guarantee it won’t react to S. agalactiae.

ab68950, ab68952, ab68954: These Staphylococcus aureus polyclonal antibodies are not absorbed for related microorganisms and so therefore could cross react with related bacteria such as Staph. Epidermidis.

MLS : Staphylococcus aureus: We do not have an antibody that is specific to Staph. aureus but does not have crossreactivity to Staph. epidermidis.

Anti-Streptococcus agalactiae:
https://www.abcam.com/ab21126 has weak crossreactivity with Strep. dysgalactiae and does not cross-react with Strep. uberis, Strep. bovis, Strep. faecalis, Staph. aureus, Mycoplasma bovis, Mycoplasma californicum, Mycoplasma bovigenitalium, E. coli or Corynebacterium bovis.

I hope this information is helpful to you. I am still waiting for more information about ab128711 (Anti-Streptococcus agalactiae antibody [224/46]).
Please do not hesitate to contact us if you need any more advice or information.

Thank you for contacting us. Indeed, neither ab68954 nor ab20920 have been tested for immunoprecipitation (IP) yet, but they could theoretically be used in this application. If it is just between the two, I would recommend ab20920 because it is an unconjugated antibody. The biotin-labeled antibody ab68954 might not bind to the Protein A or G agarose as well as the unconjugated antibody. Also, ab20920 seems to detect its antigen(s) in a rather native state (as it works with frozen tissue sections) which might be more suitable for IP. However, these antibodies are polyclonal antibodies against various soluble and structural antigens of the whole Staphylococcus aureus. By using the antibodies in IP, a variety of proteins would be pulled down. If this is not appropriate for your experimental setting, I would be pleased to assist you in finding a suitable antibody (for example against a certain target protein). If you decide to go ahead and purchase one of these products though, you might be eligible for our testing discount program. With this, you get a discount off a future purchase if you buy the antibody now, test it in IP and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.