保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
バッファーPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration information loading...
精製度Immunogen affinity purified
特記事項（精製）Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab77191 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.05 - 0.3 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 50 kDa).|
|IHC-P||Use a concentration of 1 - 2 µg/ml.|
機能Substrate of protein kinase PTK6. May play a regulatory role in the acute-phase response in systemic inflammation and may modulate STAT3 activity.
配列類似性Contains 1 PH domain.
Contains 1 SH2 domain.
翻訳後修飾Phosphorylated on tyrosine. Tyr-250 may be important for interaction with kinases. Tyr-22 and Tyr-322 appears to be phosphorylated by SRC.
- Information by UniProt
- BKS antibody
- Breast tumor kinase substrate antibody
- Brk kinase substrate antibody
Anti-STAP2 antibody (ab77191) at 0.03 µg/ml + Human heart lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 50 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Exposure time: 1 hour
ab77191 at 3.8 µg/ml staining STAP2 in Human cortex by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
ab77191 has not yet been referenced specifically in any publications.