Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7894] to SSRP1 - BSA and Azide free
- Suitable for: Flow Cyt, WB
- Reacts with: Mouse, Rat, Human
製品名Anti-SSRP1 antibody [EPR7894] - BSA and Azide free
SSRP1 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR7894] to SSRP1 - BSA and Azide free
アプリケーション適用あり: Flow Cyt, WBmore details
適用なし: ICC,ICC/IF,IHC-P or IP
種交差性交差種: Mouse, Rat, Human
Synthetic peptide within Human SSRP1 aa 50-150. The exact sequence is proprietary.
Ab248307 is the carrier-free version of ab129109. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab248307 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C. Do Not Freeze.
解離定数（KD 値）KD = 1.87 x 10 -11 M Learn more about KD
Concentration information loading...
Our Abpromise guarantee covers the use of ab248307 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).|
機能Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Binds specifically to double-stranded DNA and at low levels to DNA modified by the antitumor agent cisplatin. May potentiate cisplatin-induced cell death by blocking replication and repair of modified DNA. Also acts as a transcriptional coactivator for p63/TP63.
配列類似性Belongs to the SSRP1 family.
Contains 1 HMG box DNA-binding domain.
ドメインThe HMG box DNA-binding domain mediates DNA-binding. It has both affinity and specificity for DNA damaged globally with cisplatin.
翻訳後修飾Phosphorylated by CK2 following UV but not gamma irradiation. Phosphorylation inhibits its DNA-binding activity.
Ubiquitinated. Polyubiquitinated following caspase cleavage resulting in degradation of the N-terminal ubiquitinated part of the cleaved protein.
細胞内局在Nucleus. Chromosome. Colocalizes with RNA polymerase II on chromatin. Recruited to actively transcribed loci.
- Information by UniProt
- Chromatin-specific transcription elongation factor 80 kDa subunit antibody
- Cisplatin DNA SSRP antibody
- Facilitates chromatin remodeling 80 kDa subunit antibody
All lanes : Anti-SSRP1 antibody [EPR7894] (ab129109) at 1/10000 dilution
Lane 1 : K562 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : MOLT4 cell lysate
Lane 4 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 81 kDa
This data was developed using ab129109, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.