Application
Flow Cytometry
Fixation
Paraformaldehyde
Permeabilization
No
Sample
Human Cell (human pluripotent iPS cells)
Specification
human pluripotent iPS cells
Gating Strategy
FSC vs SSC on negative control sample
Preparation
Cell harvesting/tissue preparation method: cell dissociation buffer
Sample buffer: FACS buffer (0.2% BSA, 0.1% sodium azide in PBS)
Sample buffer: FACS buffer (0.2% BSA, 0.1% sodium azide in PBS)
Other product details
Concentration
15 µg/ml
Incubation time
45 minute(s) · Temperature: 4°C · Diluent: FACS buffer (as above)
Secondary antibody
Name
Non-Abcam antibody was used: goat anti mouse IgG PE Invitrogen
Host species: Goat
Clonality: Polyclonal
Conjugation: Phycoerythrin
Host species: Goat
Clonality: Polyclonal
Conjugation: Phycoerythrin
Dilution
1/100
Additional data
Additional Notes
This antibody gave inconclusive results. A standard flow cytometry protocol was followed, whereby cells were harvested with cell dissociation buffer and fixed in 1%PFA prior to analysis. The purple profile represents our negative control mouse IgG, the green profile is the SSEA4 antibody.
Abcam response
Thank you for sharing this data with us. ab16297 is batch tested on NTERA2 cells in flow cytometry and has been cited in numerous publications for this application. However, this review shows that ab16297 is not working on human iPS cells in flow cytometry, under these conditions. Abcam would welcome feedback from other customers currently using this antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jul 04 2013