Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human ES cell (hues7))
Specification
human ES cell (hues7)
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
1% serum 0.1% BSA in PBS as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Other product details
Dilution
1/100
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer (see above)
Secondary antibody
Name
Dilution
1/200
Additional data
Additional Notes
Undifferentiated human ES cells were fixed in 4% PFA prior to immunofluorescent analysis. SSEA3 staining (green) was observed at the cell surface of some, but not all colonies. Furthermore, evidence of 'spotting' was observed when using this antibody. Total cells were visualised using DAPI (blue).
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投稿 Jun 08 2012