Application
Flow Cytometry
Sample
Mouse Cell (embryonic stem cell (D3))
Specification
embryonic stem cell (D3)
Preparation
Cell harvesting/tissue preparation method: cell dissociation buffer
Sample buffer: enzyme-free
Sample buffer: enzyme-free
Fixation
Formaldehyde
Permeabilization
No
Gating Strategy
live cells
Other product details
Dilution
1/100
Incubation time
30 minute(s) · Temperature: 4°C · Diluent: 0.2%BSA + 0.1% sodium azide in PBS
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-mouse IgM-PE
Host species: Goat
Clonality: Polyclonal
Conjugation: Phycoerythrin
Host species: Goat
Clonality: Polyclonal
Conjugation: Phycoerythrin
Dilution
1/200
Additional data
Additional Notes
Purple is control (no primary antibody), pink line is primary and secondary antibody.
Staining is identical to another SSEA1 antibody that we have used and published with for many years.
Staining is identical to another SSEA1 antibody that we have used and published with for many years.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Nov 24 2011