製品の概要

  • 製品名
    Anti-SSEA1 antibody [MC-480]
  • 製品の詳細
    Mouse monoclonal [MC-480] to SSEA1
  • 由来種
    Mouse
  • アプリケーション
    適用あり: IHC-FoFr, ICC/IF, IP, IHC-P, IHC-FrFl, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    F9 teratocarcinoma stem cells (X-irradiated).

  • ポジティブ・コントロール
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human Hodgkin's Lymphoma Mouse embryonic carcinoma cell lines positive for SSEA1 include: F9, PCC4, ND-1, SCC1, NG2, LT/SV, MH-15, FA-25. Cell lines negative for SSEA1 include: PYS-2, OTT6050f, B3T3SV. C57SV, K129SV. KCA, QAIB, BW5147. ICC/IF: Mouse embryonic stem cells
  • 特記事項

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab16285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr Use at an assay dependent concentration.
ICC/IF 1/100.
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 1 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-FrFl Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 関連性
    Stage-specific embryonic antigen-1 (SSEA-1) was described and named as part of a series of embryonic antigens, defined by monoclonal antibodies isolated in the lab of Prof. Davor Solter and Prof. Barbara Knowles (Solter D and Knowles B.B, 1978, PNAS 75:5565). The anti SSEA-1 monoclonal antibody detects a carbohydrate epitope, which is first present on blastomeres of the 8-cell mouse embryo. It is also present on the cell surface of mouse embryonic stem (ES) cells, mouse embryonal carcinoma (EC) cells and both mouse and human embryonic germ (EG) cells. SSEA1 expression decreases as mouse ES cells differentiate but increases during the differentiation of human ES and EC cells. This antigen is also found on the cell surface of human neutrophils and myeloid leukemias, where it’s expression pattern is likened to that of CD15. However, it is currently believed that the expression pattern of SSEA1 differs from CD15, especially on the early mouse embryo.

画像

  • IHC image of SSEA1 staining in Human Hodgkin’s Lymphoma formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6.0). The section was incubated with ab16285, 1µg/ml, overnight at 4°C. A Goat anti-Mouse (IgG) HRP conjugated secondary antibody (ab97230 at 1/2000) was used to detect the primary and visualized using DAB as the chromogen. The section was counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • ab16285 stained in Mouse embryonic stem cells. Cells were fixed with 100% methanol (5min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% Triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab16285 at 5µg/ml and ab6046 (Rabbit polyclonal to beta Tubulin - Loading Control) used at 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed, (pseudo-colored green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) preadsorbed, (colored red), both used at a 1/1000 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 µM for 1hour at room temperature.

  • The image shows staining of the cell membranes of P19 mouse embryonic carcinoma cells using SSEA1-specific antibody, ab16285.
  • Cell surface flow analysis of SSEA1 on D3 mouse ES cells using ab16285 at 1:100 dilution. Purple histogram represents negative control; green line represents anti-SSEA1 antibody (ab16285).

    See Abreview

  • IHC image of SSEA1 staining in mouse normal brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16285, 10µg/ml, for 15 mins at room temperature. A Goat anti-Mouse biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

参考文献

This product has been referenced in:
  • Ortuño-Costela MDC  et al. Derivation of an aged mouse induced pluripotent stem cell line, IISHDOi005-A. Stem Cell Res 36:101418 (2019). Read more (PubMed: 30897488) »
  • Inada E  et al. Increased Expression of Cell Surface SSEA-1 is Closely Associated with Naïve-Like Conversion from Human Deciduous Teeth Dental Pulp Cells-Derived iPS Cells. Int J Mol Sci 20:N/A (2019). Read more (PubMed: 30987116) »
See all 37 Publications for this product

レビューと Q&A

1-10 of 13 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human iPSC colonies)
Permeabilization
Yes - 0.25% Triton X-100
Specification
human iPSC colonies
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde

Abcam user community

Verified customer

投稿 Jul 15 2019

Application
Immunocytochemistry/ Immunofluorescence
Sample
Chicken Cell (Chicken ESC)
Permeabilization
No
Specification
Chicken ESC
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

投稿 Jan 06 2016

Application
Immunocytochemistry
Sample
Zebrafish Cultured Cells (embryonic)
Specification
embryonic
Fixative
Formaldehyde
Permeabilization
Yes - 0.5% tritone X 100
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C

Miss. Salma Sana

Verified customer

投稿 Jan 16 2012

Answer

Thank you for your quick response.   ab119844 has been tested and characterized for ICC/IF so our Abpromise applies for this application.   If you need anything further or any help then please let me know.  

Read More

Answer

Thank you for your enquiry and your interest in our products. I can confirm that CD15 and SSEA1 are alternative names and these antibodies detect the same protein. Depending when the original antibody was produced and the clone licensed, some antobodies have the CD15 others the SSEA1 as target name. ab754 and ab119844 recognize human protein and both are suitable for immunohistochemistry on formalin-fixed paraffin-embedded (IHC-P) tissue sections. However, ab16285 only detects murine SSEA1. I am not sure what you mean by IFr application? Is it immunofluorescence (ICC/IF) or immunohistochemistry on frozen tissue (IHC-Fr) section? Could you please specify the sample types you wish to use so that I could recommend the most suitable product for you? I hope this helps and if I can assist further, please do not hesitate to contact me.  

Read More
Application
Flow Cytometry
Sample
Mouse Cell (embryonic stem cell (D3))
Specification
embryonic stem cell (D3)
Preparation
Cell harvesting/tissue preparation method: cell dissociation buffer
Sample buffer: enzyme-free
Fixation
Formaldehyde
Permeabilization
No
Gating Strategy
live cells

Abcam user community

Verified customer

投稿 Nov 24 2011

Answer

Thank you very much for your request. I have put the MSDS in the attachment of this email. The MSDS for ab19857 and ab16285 can also be directly downloaded from their datasheet. The link can be found scrolling down, underneath the "storage buffer". I hope this information is useful. Please do not hesitate to contact us again should you have any additional question.

Read More

Answer

DISCOUNT CODE: XXXXXXXXX Expiration date: 19-01-2012 I am very pleased to hear you would like to accept our offer and test ab16285 in IHC-Fr. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for IHc-Fr and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Coronal Brain Sections)
Specification
Coronal Brain Sections

Abcam user community

Verified customer

投稿 May 11 2011

Application
Flow Cytometry
Sample
Mouse Cell (mouse ES cell line D3)
Specification
mouse ES cell line D3
Preparation
Cell harvesting/tissue preparation method: cell dissociation buffer (gibco) from adherent culture
Sample buffer: FACS buffer (0.2%BSA, 0.1%sodium azide in PBS)
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
FSC vs SSC on negative control sample

Abcam user community

Verified customer

投稿 May 03 2011

1-10 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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