Anti-SSEA1 抗体 [MC-480] (ab16285)
製品の概要
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製品名Anti-SSEA1 antibody [MC-480]
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製品の詳細Mouse monoclonal [MC-480] to SSEA1
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由来種Mouse
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アプリケーション適用あり: IHC-FoFr, ICC/IF, IP, IHC-P, IHC-FrFl, Flow Cytmore details
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種交差性交差種: Mouse, Human
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免疫原
F9 teratocarcinoma stem cells (X-irradiated).
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ポジティブ･コントロール
- This antibody gave a positive result in IHC in the following FFPE tissue: Human Hodgkin's Lymphoma Mouse embryonic carcinoma cell lines positive for SSEA1 include: F9, PCC4, ND-1, SCC1, NG2, LT/SV, MH-15, FA-25. Cell lines negative for SSEA1 include: PYS-2, OTT6050f, B3T3SV. C57SV, K129SV. KCA, QAIB, BW5147.
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特記事項
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.02% Sodium azide
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精製度Tissue culture supernatant
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特記事項（精製）Tissue culture supernatant was cross flow concentrated.
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ポリ/モノモノクローナル
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クローン名MC-480
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ミエローマP3-x63-Ag8
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アイソタイプIgM
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軽鎖の種類kappa
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研究分野
関連製品
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Compatible Secondaries
- Goat F(ab')2 Anti-Mouse IgM mu chain (FITC) (ab5926)
- Goat F(ab')2 Anti-Mouse IgM mu chain (Texas Red ®) (ab5927)
- Goat F(ab')2 Anti-Mouse IgM mu chain (TRITC) (ab5928)
- Goat F(ab')2 Anti-Mouse IgM mu chain (Biotin) (ab5929)
- Goat F(ab')2 Anti-Mouse IgM mu chain (Alkaline Phosphatase) (ab5931)
- Goat F(ab')2 Anti-Mouse IgM mu chain (Phycoerythrin) (ab5932)
- Rabbit Anti-Mouse IgG+IgM+IgA H&L (ab8516)
- Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) (ab8517)
- Goat Anti-Mouse IgM H&L (ab9167)
- Rabbit Anti-Mouse IgM mu chain (ab9175)
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Conjugation kits
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Immunohistochemistry kits
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab16285 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
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| IHC-FoFr | Use at an assay dependent concentration. | |
| ICC/IF | 1/100. | |
| IP | Use at an assay dependent concentration. | |
| IHC-P | Use a concentration of 1 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| IHC-FrFl | Use at an assay dependent concentration. | |
| Flow Cyt | Use at an assay dependent concentration. ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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関連性Stage-specific embryonic antigen-1 (SSEA-1) was described and named as part of a series of embryonic antigens, defined by monoclonal antibodies isolated in the lab of Prof. Davor Solter and Prof. Barbara Knowles (Solter D and Knowles B.B, 1978, PNAS 75:5565). The anti SSEA-1 monoclonal antibody detects a carbohydrate epitope, which is first present on blastomeres of the 8-cell mouse embryo. It is also present on the cell surface of mouse embryonic stem (ES) cells, mouse embryonal carcinoma (EC) cells and both mouse and human embryonic germ (EG) cells. SSEA1 expression decreases as mouse ES cells differentiate but increases during the differentiation of human ES and EC cells. This antigen is also found on the cell surface of human neutrophils and myeloid leukemias, where it’s expression pattern is likened to that of CD15. However, it is currently believed that the expression pattern of SSEA1 differs from CD15, especially on the early mouse embryo.
画像
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSEA1 antibody [MC-480] (ab16285)](https://www.abcam.co.jp/ps/products/16/ab16285/Images/ab16285-346812-ab16285ap4507114Huhodgekinslymphoma5ugedited.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSEA1 antibody [MC-480] (ab16285)IHC image of SSEA1 staining in Human Hodgkin’s Lymphoma formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6.0). The section was incubated with ab16285, 1µg/ml, overnight at 4°C. A Goat anti-Mouse (IgG) HRP conjugated secondary antibody (ab97230 at 1/2000) was used to detect the primary and visualized using DAB as the chromogen. The section was counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Immunocytochemistry/ Immunofluorescence - Anti-SSEA1 antibody [MC-480] (ab16285)](https://www.abcam.co.jp/ps/products/16/ab16285/Images/ap200085-ab16285-081001-1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SSEA1 antibody [MC-480] (ab16285)ICC/IF image of ab16285 stained mouse embryonic stem cells. The cells were 4% formalin fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16285, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgM (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). -
![Immunocytochemistry/ Immunofluorescence - Anti-SSEA1 antibody [MC-480] (ab16285)](https://www.abcam.co.jp/ps/products/16/ab16285/Images/ab16285_2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SSEA1 antibody [MC-480] (ab16285)This image is courtesy of the laboratory of Peter Andrews.The image shows staining of the cell membranes of P19 mouse embryonic carcinoma cells using SSEA1-specific antibody, ab16285. -
![Flow Cytometry - Anti-SSEA1 antibody [MC-480] (ab16285)](https://www.abcam.co.jp/ps/products/16/ab16285/Images/SSEA1-Primary-antibodies-ab16285-6.jpg)
Flow Cytometry - Anti-SSEA1 antibody [MC-480] (ab16285)This image is taken from an anonymous abreview.Cell surface flow analysis of SSEA1 on D3 mouse ES cells using ab16285 at 1:100 dilution. Purple histogram represents negative control; green line represents anti-SSEA1 antibody (ab16285). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSEA1 antibody [MC-480] (ab16285)](https://www.abcam.co.jp/ps/products/16/ab16285/Images/SSEA1-Primary-antibodies-ab16285-15.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSEA1 antibody [MC-480] (ab16285)IHC image of SSEA1 staining in mouse normal brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16285, 10µg/ml, for 15 mins at room temperature. A Goat anti-Mouse biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
参考文献
This product has been referenced in:
- Pei Y et al. Murine pluripotent stem cells that escape differentiation inside teratomas maintain pluripotency. PeerJ 6:e4177 (2018). Read more (PubMed: 29312817) »
- Wu Y et al. Generation and characterization of induced pluripotent stem cells from guinea pig fetal fibroblasts. Mol Med Rep 15:3690-3698 (2017). Read more (PubMed: 28393187) »
