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    ssea1-antibody-mc-480-ab16285.pdf

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Anti-SSEA1 抗体 [MC-480] (ab16285)

  • Datasheet
  • SDS
Reviews (9)Q&A (4)References (42)

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出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSEA1 antibody [MC-480] (ab16285)
  • Immunocytochemistry/ Immunofluorescence - Anti-SSEA1 antibody [MC-480] (ab16285)
  • Immunocytochemistry/ Immunofluorescence - Anti-SSEA1 antibody [MC-480] (ab16285)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSEA1 antibody [MC-480] (ab16285)

Key features and details

  • Mouse monoclonal [MC-480] to SSEA1
  • Suitable for: IHC-FoFr, ICC/IF, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgM

こちらの製品もご検討ください

Biochemical
AG 490, JAK2/3 inhibitor (ab120950)

関連製品

製品の概要

  • 製品名

    Anti-SSEA1 antibody [MC-480]
  • 製品の詳細

    Mouse monoclonal [MC-480] to SSEA1
  • 由来種

    Mouse
  • アプリケーション

    適用あり: IHC-FoFr, ICC/IF, IHC-Pmore details
  • 種交差性

    交差種: Mouse, Human
  • 免疫原

    Tissue, cells or virus corresponding to Mouse SSEA1. The mouse was immunized with F9 teratocarcinoma cells

  • ポジティブ・コントロール

    • This antibody gave a positive result in IHC in the following FFPE tissue: Human Hodgkin's Lymphoma Mouse embryonic carcinoma cell lines positive for SSEA1 include: F9, PCC4, ND-1, SCC1, NG2, LT/SV, MH-15, FA-25. Cell lines negative for SSEA1 include: PYS-2, OTT6050f, B3T3SV. C57SV, K129SV. KCA, QAIB, BW5147. ICC/IF: Mouse embryonic stem cells
  • 特記事項

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • 精製度

    Tissue culture supernatant
  • 特記事項(精製)

    Tissue culture supernatant was cross flow concentrated.
  • ポリ/モノ

    モノクローナル
  • クローン名

    MC-480
  • ミエローマ

    P3-x63-Ag8
  • アイソタイプ

    IgM
  • 軽鎖の種類

    kappa
  • 研究分野

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Immunology
    • Cell Type Markers
    • CD
    • Adhesion
    • Stem Cells
    • Embryonic Stem Cells
    • Surface Molecules
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • Human Lineage Negative
    • Stem Cells
    • Mesenchymal Stem Cells
    • Surface Molecules
    • Cancer
    • Tumor immunology
    • CD markers
    • Stem Cells
    • Endothelial Progenitors
    • Endothelial Markers
    • Developmental Biology
    • Embryogenesis
    • Embryonic stem cells
    • Surface molecules
    • Developmental Biology
    • Embryogenesis
    • Embryonic stem cells
    • Intracellular

関連製品

  • Compatible Secondaries

    • Goat F(ab')2 Anti-Mouse IgM mu chain (FITC) (ab5926)
    • Goat F(ab')2 Anti-Mouse IgM mu chain (Texas Red ®) (ab5927)
    • Goat F(ab')2 Anti-Mouse IgM mu chain (TRITC) (ab5928)
    • Goat F(ab')2 Anti-Mouse IgM mu chain (Biotin) (ab5929)
    • Goat F(ab')2 Anti-Mouse IgM mu chain (PE) (ab5932)
    • Rabbit Anti-Mouse IgG+IgM+IgA H&L (ab8516)
    • Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) (ab8517)
    • Goat Anti-Mouse IgM H&L (ab9167)
    • Rabbit Anti-Mouse IgM mu chain (ab9175)
  • Isotype control

    • Mouse IgM [B11/7] - Isotype control (ab91545)

アプリケーション

Our Abpromise guarantee covers the use of ab16285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 - 10 µg/ml.

Fixation with 100% MeOH (5 min) or 4% PFA (10 min).

IHC-P 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 関連性

    Stage-specific embryonic antigen-1 (SSEA-1) was described and named as part of a series of embryonic antigens, defined by monoclonal antibodies isolated in the lab of Prof. Davor Solter and Prof. Barbara Knowles (Solter D and Knowles B.B, 1978, PNAS 75:5565). The anti SSEA-1 monoclonal antibody detects a carbohydrate epitope, which is first present on blastomeres of the 8-cell mouse embryo. It is also present on the cell surface of mouse embryonic stem (ES) cells, mouse embryonal carcinoma (EC) cells and both mouse and human embryonic germ (EG) cells. SSEA1 expression decreases as mouse ES cells differentiate but increases during the differentiation of human ES and EC cells. This antigen is also found on the cell surface of human neutrophils and myeloid leukemias, where it’s expression pattern is likened to that of CD15. However, it is currently believed that the expression pattern of SSEA1 differs from CD15, especially on the early mouse embryo.

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSEA1 antibody [MC-480] (ab16285)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSEA1 antibody [MC-480] (ab16285)

    IHC image of SSEA1 staining in Human Hodgkin’s Lymphoma formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6.0). The section was incubated with ab16285, 1/200 dilution, overnight at 4°C. A Goat anti-Mouse (IgM) HRP conjugated secondary antibody (ab97230 at 1/2000) was used to detect the primary and visualized using DAB as the chromogen. The section was counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Immunocytochemistry/ Immunofluorescence - Anti-SSEA1 antibody [MC-480] (ab16285)
    Immunocytochemistry/ Immunofluorescence - Anti-SSEA1 antibody [MC-480] (ab16285)

    ab16285 stained in Mouse embryonic stem cells. Cells were fixed with 100% methanol (5min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% Triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab16285 at 5µg/ml and ab6046 (Rabbit polyclonal to beta Tubulin - Loading Control) used at 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed, (pseudo-colored green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) preadsorbed, (colored red), both used at a 1/1000 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 µM for 1hour at room temperature.

  • Immunocytochemistry/ Immunofluorescence - Anti-SSEA1 antibody [MC-480] (ab16285)
    Immunocytochemistry/ Immunofluorescence - Anti-SSEA1 antibody [MC-480] (ab16285)This image is courtesy of the laboratory of Peter Andrews.
    The image shows staining of the cell membranes of P19 mouse embryonic carcinoma cells using SSEA1-specific antibody, ab16285.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSEA1 antibody [MC-480] (ab16285)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSEA1 antibody [MC-480] (ab16285)

    IHC image of SSEA1 staining in mouse normal brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16285, 10µg/ml, for 15 mins at room temperature. A Goat anti-Mouse biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

プロトコール

  • Mouse on Mouse staining protocol

Click here to view the general protocols

データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (42)

    ab16285 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab16285 は 42 報の論文で使用されています。

    • Liu C  et al. Identification of primordial germ cell-like cells as liver metastasis initiating cells in mouse tumour models. Cell Discov 6:15 (2020). IHC ; Mouse . PubMed: 32218989
    • Chen K  et al. Spontaneously Formed Spheroids from Mouse Compact Bone-Derived Cells Retain Highly Potent Stem Cells with Enhanced Differentiation Capability. Stem Cells Int 2019:8469012 (2019). ICC/IF ; Mouse . PubMed: 31191686
    • Ortuño-Costela MDC  et al. Derivation of an aged mouse induced pluripotent stem cell line, IISHDOi005-A. Stem Cell Res 36:101418 (2019). ICC/IF ; Mouse . PubMed: 30897488
    • Inada E  et al. Increased Expression of Cell Surface SSEA-1 is Closely Associated with Naïve-Like Conversion from Human Deciduous Teeth Dental Pulp Cells-Derived iPS Cells. Int J Mol Sci 20:N/A (2019). ICC/IF . PubMed: 30987116
    • Li N  et al. Characterization of spontaneous spheroids from oral mucosa-derived cells and their direct comparison with spheroids from skin-derived cells. Stem Cell Res Ther 10:184 (2019). ICC/IF . PubMed: 31234925
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 13 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-SSEA1 antibody [MC-480]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (human iPSC colonies)
    Permeabilization
    Yes - 0.25% Triton X-100
    Specification
    human iPSC colonies
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 15 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-SSEA1 antibody [MC-480]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Chicken Cell (Chicken ESC)
    Permeabilization
    No
    Specification
    Chicken ESC
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Jan 06 2016

    Immunocytochemistry abreview for Anti-SSEA1 antibody [MC480]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Zebrafish Cultured Cells (embryonic)
    Specification
    embryonic
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.5% tritone X 100
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C
    Read More

    Miss. Salma Sana

    Verified customer

    投稿 Jan 16 2012

    Question

    Thank you for your answer The application I will use is the immunofluorescence on PFA fixed cells  

    Read More

    Abcam community

    Verified customer

    Asked on Nov 24 2011

    Answer

    Thank you for your quick response.   ab119844 has been tested and characterized for ICC/IF so our Abpromise applies for this application.   If you need anything further or any help then please let me know.  

    Read More

    Abcam Scientific Support

    Answered on Nov 24 2011

    Question

    Dear Technical Support I have questions about anti CD15 antibodies. CD15 is synonymous with SSEA-1 but you have to different set of antibodies: CD15 (ab119844 or ab754) and SSEA-1 (ab16285). Do they recognize the same protein? I need Ab against human protein, does the ab16285 react with human? If not may I buy a CD15 antibody to stain the same cells? Which is better for IFr application, the ab119844 or ab754 Thank ou very much  

    Read More

    Abcam community

    Verified customer

    Asked on Nov 24 2011

    Answer

    Thank you for your enquiry and your interest in our products. I can confirm that CD15 and SSEA1 are alternative names and these antibodies detect the same protein. Depending when the original antibody was produced and the clone licensed, some antobodies have the CD15 others the SSEA1 as target name. ab754 and ab119844 recognize human protein and both are suitable for immunohistochemistry on formalin-fixed paraffin-embedded (IHC-P) tissue sections. However, ab16285 only detects murine SSEA1. I am not sure what you mean by IFr application? Is it immunofluorescence (ICC/IF) or immunohistochemistry on frozen tissue (IHC-Fr) section? Could you please specify the sample types you wish to use so that I could recommend the most suitable product for you? I hope this helps and if I can assist further, please do not hesitate to contact me.  

    Read More

    Abcam Scientific Support

    Answered on Nov 24 2011

    Flow Cytometry abreview for Anti-SSEA1 antibody [MC480]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Mouse Cell (embryonic stem cell (D3))
    Specification
    embryonic stem cell (D3)
    Preparation
    Cell harvesting/tissue preparation method: cell dissociation buffer
    Sample buffer: enzyme-free
    Fixation
    Formaldehyde
    Permeabilization
    No
    Gating Strategy
    live cells
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 24 2011

    Question

    Hello,I need MSDS for the following products ab19857 and ab16285. Can you kindly email them to me.Thank YouRegards,

    Read More

    Abcam community

    Verified customer

    Asked on Oct 05 2011

    Answer

    Thank you very much for your request. I have put the MSDS in the attachment of this email. The MSDS for ab19857 and ab16285 can also be directly downloaded from their datasheet. The link can be found scrolling down, underneath the "storage buffer". I hope this information is useful. Please do not hesitate to contact us again should you have any additional question.

    Read More

    Abcam Scientific Support

    Answered on Oct 05 2011

    Question

    Dear Tech Support Team,  Our customer is willing to test ab16285 in IHC-Fr with mouse sections. Are you interested in this type of the information for the Abreview promotion (The customer buys certain antibodies and upload Abreview on Abcam's website and then we give 100% credit note for this antibody to buy next antibody)? Thanks in advance for your assistance and reply. Have a nice week,

    Read More

    Abcam community

    Verified customer

    Asked on Sep 19 2011

    Answer

    DISCOUNT CODE: XXXXXXXXX Expiration date: 19-01-2012 I am very pleased to hear you would like to accept our offer and test ab16285 in IHC-Fr. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for IHc-Fr and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Sep 19 2011

    Immunohistochemistry - Free Floating abreview for Anti-SSEA1 antibody [MC480]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Mouse Tissue sections (Coronal Brain Sections)
    Specification
    Coronal Brain Sections
    Read More

    Abcam user community

    Verified customer

    投稿 May 11 2011

    Flow Cytometry abreview for Anti-SSEA1 antibody [MC480]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Mouse Cell (mouse ES cell line D3)
    Specification
    mouse ES cell line D3
    Preparation
    Cell harvesting/tissue preparation method: cell dissociation buffer (gibco) from adherent culture
    Sample buffer: FACS buffer (0.2%BSA, 0.1%sodium azide in PBS)
    Fixation
    Paraformaldehyde
    Permeabilization
    No
    Gating Strategy
    FSC vs SSC on negative control sample
    Read More

    Abcam user community

    Verified customer

    投稿 May 03 2011

    1-10 of 13 Abreviews or Q&A

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