Anti-SSEA1 抗体 [MC-480] (ab16285)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSEA1 antibody [MC-480] (ab16285)](https://www.abcam.co.jp/ps/products/16/ab16285/Images/ab16285-384712-ap3698875assy76353datasheetimage.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSEA1 antibody [MC-480] (ab16285)")
Key features and details
- Mouse monoclonal [MC-480] to SSEA1
- Suitable for: IHC-FoFr, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgM
製品の概要
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製品名
Anti-SSEA1 antibody [MC-480] -
製品の詳細
Mouse monoclonal [MC-480] to SSEA1 -
由来種
Mouse -
アプリケーション
適用あり: IHC-FoFr, ICC/IF, IHC-Pmore details -
種交差性
交差種: Mouse, Human -
免疫原
Tissue, cells or virus corresponding to Mouse SSEA1. The mouse was immunized with F9 teratocarcinoma cells
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ポジティブ･コントロール
- This antibody gave a positive result in IHC in the following FFPE tissue: Human Hodgkin's Lymphoma Mouse embryonic carcinoma cell lines positive for SSEA1 include: F9, PCC4, ND-1, SCC1, NG2, LT/SV, MH-15, FA-25. Cell lines negative for SSEA1 include: PYS-2, OTT6050f, B3T3SV. C57SV, K129SV. KCA, QAIB, BW5147. ICC/IF: Mouse embryonic stem cells
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特記事項
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Sodium azide -
Concentration information loading... -
精製度
Tissue culture supernatant -
特記事項（精製）
Tissue culture supernatant was cross flow concentrated. -
ポリ/モノ
モノクローナル -
クローン名
MC-480 -
ミエローマ
P3-x63-Ag8 -
アイソタイプ
IgM -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
- Goat F(ab')2 Anti-Mouse IgM mu chain (FITC) (ab5926)
- Goat F(ab')2 Anti-Mouse IgM mu chain (Texas Red ®) (ab5927)
- Goat F(ab')2 Anti-Mouse IgM mu chain (TRITC) (ab5928)
- Goat F(ab')2 Anti-Mouse IgM mu chain (Biotin) (ab5929)
- Goat F(ab')2 Anti-Mouse IgM mu chain (PE) (ab5932)
- Rabbit Anti-Mouse IgG+IgM+IgA H&L (ab8516)
- Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) (ab8517)
- Goat Anti-Mouse IgM H&L (ab9167)
- Rabbit Anti-Mouse IgM mu chain (ab9175)
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab16285 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-FoFr | Use at an assay dependent concentration. | |
| ICC/IF | Use a concentration of 5 - 10 µg/ml. Fixation with 100% MeOH (5 min) or 4% PFA (10 min). |
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| IHC-P | 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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関連性
Stage-specific embryonic antigen-1 (SSEA-1) was described and named as part of a series of embryonic antigens, defined by monoclonal antibodies isolated in the lab of Prof. Davor Solter and Prof. Barbara Knowles (Solter D and Knowles B.B, 1978, PNAS 75:5565). The anti SSEA-1 monoclonal antibody detects a carbohydrate epitope, which is first present on blastomeres of the 8-cell mouse embryo. It is also present on the cell surface of mouse embryonic stem (ES) cells, mouse embryonal carcinoma (EC) cells and both mouse and human embryonic germ (EG) cells. SSEA1 expression decreases as mouse ES cells differentiate but increases during the differentiation of human ES and EC cells. This antigen is also found on the cell surface of human neutrophils and myeloid leukemias, where it’s expression pattern is likened to that of CD15. However, it is currently believed that the expression pattern of SSEA1 differs from CD15, especially on the early mouse embryo.
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSEA1 antibody [MC-480] (ab16285)
IHC image of SSEA1 staining in Human Hodgkin’s Lymphoma formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6.0). The section was incubated with ab16285, 1/200 dilution, overnight at 4°C. A Goat anti-Mouse (IgM) HRP conjugated secondary antibody (ab97230 at 1/2000) was used to detect the primary and visualized using DAB as the chromogen. The section was counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Immunocytochemistry/ Immunofluorescence - Anti-SSEA1 antibody [MC-480] (ab16285)](https://www.abcam.co.jp/ps/products/16/ab16285/Images/ab16285-352441-antiSSEA1antibodyimmunocytochemistrymESmouse.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SSEA1 antibody [MC-480] (ab16285)ab16285 stained in Mouse embryonic stem cells. Cells were fixed with 100% methanol (5min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% Triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab16285 at 5µg/ml and ab6046 (Rabbit polyclonal to beta Tubulin - Loading Control) used at 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed, (pseudo-colored green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) preadsorbed, (colored red), both used at a 1/1000 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 µM for 1hour at room temperature.
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![Immunocytochemistry/ Immunofluorescence - Anti-SSEA1 antibody [MC-480] (ab16285)](https://www.abcam.co.jp/ps/products/16/ab16285/Images/ab16285-24919-anti-ssea1-antibody-mc-480-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SSEA1 antibody [MC-480] (ab16285)This image is courtesy of the laboratory of Peter Andrews.The image shows staining of the cell membranes of P19 mouse embryonic carcinoma cells using SSEA1-specific antibody, ab16285. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSEA1 antibody [MC-480] (ab16285)](https://www.abcam.co.jp/ps/products/16/ab16285/Images/ab16285-24917-anti-ssea1-antibody-mc-480-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSEA1 antibody [MC-480] (ab16285)IHC image of SSEA1 staining in mouse normal brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16285, 10µg/ml, for 15 mins at room temperature. A Goat anti-Mouse biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
参考文献 (42)
ab16285 は 42 報の論文で使用されています。
- Liu C et al. Identification of primordial germ cell-like cells as liver metastasis initiating cells in mouse tumour models. Cell Discov 6:15 (2020). IHC ; Mouse . PubMed: 32218989
- Chen K et al. Spontaneously Formed Spheroids from Mouse Compact Bone-Derived Cells Retain Highly Potent Stem Cells with Enhanced Differentiation Capability. Stem Cells Int 2019:8469012 (2019). ICC/IF ; Mouse . PubMed: 31191686
- Ortuño-Costela MDC et al. Derivation of an aged mouse induced pluripotent stem cell line, IISHDOi005-A. Stem Cell Res 36:101418 (2019). ICC/IF ; Mouse . PubMed: 30897488
- Inada E et al. Increased Expression of Cell Surface SSEA-1 is Closely Associated with Naïve-Like Conversion from Human Deciduous Teeth Dental Pulp Cells-Derived iPS Cells. Int J Mol Sci 20:N/A (2019). ICC/IF . PubMed: 30987116
- Li N et al. Characterization of spontaneous spheroids from oral mucosa-derived cells and their direct comparison with spheroids from skin-derived cells. Stem Cell Res Ther 10:184 (2019). ICC/IF . PubMed: 31234925
