Anti-SRP68 抗体 (ab157120)
Key features and details
- Rabbit polyclonal to SRP68
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-SRP68 antibody
SRP68 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SRP68 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Opossum, Common marmoset -
免疫原
Synthetic peptide, corresponding to a region within the N terminal amino acids 1-50 of Human SRP68 (NP_055045.2).
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ポジティブ・コントロール
- HeLa, 293T, TCMK 1, Renca, NIH 3T3, and Jurkat whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab157120 was affinity purified using an epitope specific to SRP68 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab157120の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 70 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 70 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
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機能
Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. SRP68 binds the 7S RNA, SRP72 binds to this complex subsequently. This ribonucleoprotein complex might interact directly with the docking protein in the ER membrane and possibly participate in the elongation arrest function. -
配列類似性
Belongs to the SRP68 family. -
ドメイン
The RNA-binding domain is located near the N-terminus. -
細胞内局在
Cytoplasm. Nucleus > nucleolus. - Information by UniProt
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参照データベース
- Entrez Gene: 6730 Human
- Entrez Gene: 217337 Mouse
- Omim: 604858 Human
- SwissProt: Q00004 Dog
- SwissProt: Q9UHB9 Human
- SwissProt: Q8BMA6 Mouse
- Unigene: 514495 Human
- Unigene: 29655 Mouse
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別名
- Signal recognition particle 68 antibody
- Signal recognition particle 68 kDa antibody
- Signal recognition particle 68 kDa protein antibody
see all
画像
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All lanes : Anti-SRP68 antibody (ab157120) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 70 kDa
Exposure time: 30 seconds -
All lanes : Anti-SRP68 antibody (ab157120) at 0.1 µg/ml
Lane 1 : TCMK 1 whole cell lysate
Lane 2 : Renca whole cell lysate
Lane 3 : NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 70 kDa
Exposure time: 30 seconds -
Detection of SRP68 by Western Blot of Immunprecipitate.
ab157120 at 0.4µg/ml labeling SRP68 in HeLa whole cell lysate immunoprecipitated using ab157120 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab157120 は論文での使用が確認できていません。