Anti-srGAP2 抗体 [EP2506(2)] - BSA and Azide free (ab248072)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2506(2)] to srGAP2 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-srGAP2 antibody [EP2506(2)] - BSA and Azide free
srGAP2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP2506(2)] to srGAP2 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF,IHC-P or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: HEK-293, and Mouse and Rat brain lysate.
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特記事項
ab248072 is the carrier-free version of ab124958.
This product has switched from a hybridoma to recombinant production method on 7th September 2023.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.20
Constituent: 100% PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP2506(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab248072の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 140 kDa (predicted molecular weight: 121 kDa).
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特記事項 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 140 kDa (predicted molecular weight: 121 kDa). |
ターゲット情報
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機能
Putative GTPase-activating protein for Rho family small GTPases. -
組織特異性
Expression at low levels in placenta, kidney, and ovary, and little or no expression in all other tissues tested. -
配列類似性
Contains 1 FCH domain.
Contains 1 Rho-GAP domain.
Contains 1 SH3 domain. - Information by UniProt
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参照データベース
- Entrez Gene: 23380 Human
- Entrez Gene: 14270 Mouse
- Entrez Gene: 360840 Rat
- Omim: 606524 Human
- SwissProt: O75044 Human
- SwissProt: Q91Z67 Mouse
- SwissProt: D4A208 Rat
- Unigene: 497575 Human
see all -
別名
- ARHGAP34 antibody
- FBP2 antibody
- FNBP2 antibody
see all
画像
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Anti-srGAP2 antibody [EP2506(2)] (ab124958) at 1/1000 dilution + HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 121 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
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All lanes : Anti-srGAP2 antibody [EP2506(2)] (ab124958) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 121 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab248072 は論文での使用が確認できていません。