Anti-SREBP1 抗体 [2A4] - BSA and Azide free (ab269577)

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Western blot - Anti-SREBP1 antibody [2A4] - BSA and Azide free (ab269577)

    Key features and details

    • Mouse monoclonal [2A4] to SREBP1 - BSA and Azide free
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG1

    製品の概要

    • 製品名

      Anti-SREBP1 antibody [2A4] - BSA and Azide free
      SREBP1 一次抗体 製品一覧

    • 製品の詳細

      Mouse monoclonal [2A4] to SREBP1 - BSA and Azide free

    • 由来種

      Mouse

    • Tested Applications & Species

      Application Species
      Protein Array
      Human
      See all applications and species data

    • 免疫原

      Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

    • エピトープ

      Amino acids 301 - 407.

    • ポジティブ･コントロール

      • WB: HeLa and HAP1 whole cell lysates.

    • 特記事項

      This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

      ab269577 is the carrier-free version of ab3259. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

       

      Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

      Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

      This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

      Maxpar® is a trademark of Fluidigm Canada Inc.

    アプリケーション

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab269577 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    アプリケーション Species
    Protein Array
    Human
    アプリケーション Abreviews 特記事項
    WB
    Use a concentration of 5 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 127 kDa).

    By Western blot, this antibody detects bands of 125 kDa (precursor SREBP1) and 60 - 70 kDa (cleaved SREBP1). 

    Based on in-house testing, Abcam recommends using a 5% milk block for this product. 
    特記事項
    WB
    Use a concentration of 5 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 127 kDa).

    By Western blot, this antibody detects bands of 125 kDa (precursor SREBP1) and 60 - 70 kDa (cleaved SREBP1). 

    Based on in-house testing, Abcam recommends using a 5% milk block for this product. 

    ターゲット情報

    • 機能

      Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway (By similarity). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3').

    • 組織特異性

      Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines. Isoform SREBP-1A and isoform SREBP-1C are found in kidney, brain, white fat, and muscle.

    • 配列類似性

      Belongs to the SREBP family.
      Contains 1 basic helix-loop-helix (bHLH) domain.

    • 翻訳後修飾

      At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7.
      Phosphorylated by AMPK, leading to suppress protein processing and nuclear translocation, and repress target gene expression. Phosphorylation at Ser-402 by SIK1 represses activity possibly by inhibiting DNA-binding.

    • 細胞内局在

      Nucleus and Endoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasmic vesicle > COPII-coated vesicle membrane. Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.
    • Information by UniProt

    • 参照データベース

      • 別名

        • ADD 1 antibody
        • bHLHd1 antibody
        • Class D basic helix-loop-helix protein 1 antibody
        • D630008H06 antibody
        • Processed sterol regulatory element-binding protein 1 antibody
        • SRBP1_HUMAN antibody
        • SREBF 1 antibody
        • SREBF1 antibody
        • SREBP 1 antibody
        • SREBP 1c antibody
        • SREBP-1 antibody
        • SREBP1 antibody
        • Sterol regulatory element binding protein 1 antibody
        • Sterol Regulatory Element Binding Transcription Factor 1 / Protein 1 antibody
        • Sterol regulatory element binding transcription factor 1 antibody
        • Sterol regulatory element-binding transcription factor 1 antibody
        see all

      画像

      • Western blot - Anti-SREBP1 antibody [2A4] - BSA and Azide free (ab269577)
        Western blot - Anti-SREBP1 antibody [2A4] - BSA and Azide free (ab269577)
        All lanes : Anti-SREBP1 antibody [2A4] (ab3259) at 5 µg/ml

        Lane 1 : HeLa whole cell lysate
        Lane 2 : HAP1 whole cell lysate

        Lysates/proteins at 20 µg per lane.

        Performed under reducing conditions.

        Predicted band size: 127 kDa



        This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was blocked for an hour using 5% milk before ab3259 and ab181602 (rabbit anti-GAPDH loading control) were incubated overnight at 4°C at a 5ug/ml concentration and 1/20000 dilution respectively. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

        This image was produced using the same antibody clone but in a different formulation ab3259, PBS and sodium azide.

      プロトコール

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      参考文献 (0)

      ab269577 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab269577 は論文での使用が確認できていません。

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