製品の概要

  • 製品名

    SREBP-2 Transcription Factor Assay Kit
  • 検出方法

    Colorimetric
  • サンプルの種類

    Adherent cells, Suspension cells, Nuclear Extracts
  • アッセイタイプ

    Semi-quantitative
  • 種交差性

    交差種: Human
    交差が予測される動物種: Mammals
  • 製品の概要

    Abcam's SREBP-2 Transcription Factor Assay Kit (ab133111) is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates.

    A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the SREBP response element is immobilized to the wells of a 96-well plate. SREBP contained in a nuclear extract, binds specifically to the SREBP response element. SREBP is detected by addition of a specific primary antibody directed against SREBP. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm.

  • 特記事項

    Lipid homeostasis in vertebrate cells is regulated by a family of transcription factors called sterol regulatory elements binding proteins (SREBPs). SREBPs directly activate the expression of over 30 gened involved in teh synthesis and uptake of cholesterol, fatty cids, triglycerides and phopholipids. SREBP-2 activates cholesterol synthesis by upregulating expression of HMG-CoA reductase.

  • 試験プラットフォーム

    Microplate reader

製品の特性

関連製品

画像

  • HepG2 cells were grown in protein free hybridoma medium overnight (Life Technologies), followed by a 30 minutes incubation with the addition of 1% (2-Hydroxypropyl)-β-cyclodextrin (Sigma) or with the supplement of liqued cholesterol (Life Technologies). 10 uL of nuclear extracts (ab113474) were tested, corresponding to 2.5e5 cells per well.

  • Positive control (10 or 5 uL) with or without inhibitor (10 or 2 uL) (duplicates; +/- SD).

  • Example of typical standard curve obtained using ab133111.

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参考文献

This product has been referenced in:

  • Marwarha G  et al. Palmitate-Induced SREBP1 Expression and Activation Underlies the Increased BACE 1 Activity and Amyloid Beta Genesis. Mol Neurobiol N/A:N/A (2018). Read more (PubMed: 30569418) »
See 1 Publication for this product

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