Application
Western blot
Sample
Human Cell lysate - whole cell (Hela kyoto beta)
Loading amount
15 µg
Specification
Hela kyoto beta
Treatment
siRNA knock down for 48 h
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 8% · Temperature: 4°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBST +4% Milk
Secondary antibody
Name
Non-Abcam antibody was used: ECL anti-rabbit IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 60 kDa
Positive control
MOCK
Negative control
siRNA knock down cells
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Aug 05 2010