Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (Mouse Embyronic fibroblasts)
Total protein in input
200 µg
Immuno-precipitation step
Protein A
Specification
Mouse Embyronic fibroblasts
Other product details
Incubation time
1 hour(s) and 30 minute(s) · Temperature: 4°C · Diluent: Standard NP40 lysis buffer
Dilution
1/150
Western Blot antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: anti-p62
Host species: Guinea pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Guinea pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
Important note! We found that this antibody does not recognize denatured mouse p62. However, we found that it IP's the native protein quite well. We have verified that this antibody works well for IP but is unsuitable for mouse Western, but Western works for human p62. Hope this saves you some troubleshooting!
Abcam response
Thank you for your valuable feedback. We hope that this information will be helpful for future customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Aug 22 2017