Application
Western blot
Sample
Mouse Cell lysate - whole cell (CAFs)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
50 µg
Specification
CAFs
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Milk (5%) in TBS-T
Dilution
1/1000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: anti rb HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
shRNA against murined p62
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 62 kDa
Positive control
samples
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jul 06 2020