Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5869] to SPTBN1 - BSA and Azide free
- Suitable for: ICC, WB, IHC-P
- Reacts with: Mouse, Rat, Human
製品名Anti-SPTBN1 antibody [EPR5869] - BSA and Azide free
SPTBN1 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR5869] to SPTBN1 - BSA and Azide free
アプリケーション適用あり: ICC, WB, IHC-Pmore details
適用なし: Flow Cyt or IP
種交差性交差種: Mouse, Rat, Human
Synthetic peptide within Human SPTBN1 aa 1900-2000. The exact sequence is proprietary.
Ab248042 is the carrier-free version of ab124888. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab248042 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C. Do Not Freeze.
解離定数（KD 値）KD = 4.40 x 10 -11 M Learn more about KD
Concentration information loading...
Our Abpromise guarantee covers the use of ab248042 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 270 kDa (predicted molecular weight: 275 kDa).|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
For antigen retrieval, heat up to 98 degree C, below boiling, and then let cool for 10-20 minutes.
機能Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
組織特異性Isoform 2 is present in brain, lung and kidney (at protein level).
配列類似性Belongs to the spectrin family.
Contains 2 CH (calponin-homology) domains.
Contains 1 PH domain.
Contains 17 spectrin repeats.
翻訳後修飾Isoform 2 is phosphorylated on Ser-8 and Ser-10.
細胞内局在Cell membrane and Cytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere > M line. Colocalizes with ANK2 in a distinct intracellular compartment of neonatal cardiomyocytes.
- Information by UniProt
- Beta G spectrin antibody
- Beta II spectrin antibody
- Beta spectrin 2 antibody
All lanes : Anti-SPTBN1 antibody [EPR5869] (ab124888) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Caco 2 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 275 kDa
This data was developed using ab124888, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.