Anti-Spt6 抗体 (ab32820)
Key features and details
- Rabbit polyclonal to Spt6
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Spt6 antibody -
製品の詳細
Rabbit polyclonal to Spt6 -
由来種
Rabbit -
特異性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Zebrafish -
免疫原
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ポジティブ・コントロール
- This antibody gave a positive signal for the following cell lysates: HeLa Nuclear; HeLa; Jurkat.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab32820の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
Use at an assay dependent concentration.
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WB | (1) |
1/500. Detects a band of approximately 230 kDa (predicted molecular weight: 199 kDa).
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特記事項 |
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IP
Use at an assay dependent concentration. |
WB
1/500. Detects a band of approximately 230 kDa (predicted molecular weight: 199 kDa). |
ターゲット情報
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機能
Acts to stimulate transcriptional elongation by RNA polymerase II. -
組織特異性
Ubiquitously expressed. -
配列類似性
Belongs to the SPT6 family.
Contains 1 S1 motif domain.
Contains 1 SH2 domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 6830 Human
- Entrez Gene: 20926 Mouse
- Entrez Gene: 337866 Zebrafish
- Omim: 601333 Human
- SwissProt: Q7KZ85 Human
- SwissProt: Q3TY72 Mouse
- SwissProt: Q62383 Mouse
- SwissProt: Q8UVK2 Zebrafish
see all -
別名
- emb 5 antibody
- hSPT6 antibody
- KIAA0162 antibody
see all
画像
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Lane 1 : Marker
Lanes 2-4 : Anti-Spt6 antibody (ab32820) at 1/500 dilution
Lane 5 : High Mark marker
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 3 :Jurkat whole cell lysate (ab7899) at 20 µg
Lane 4 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 20 µg
Secondary
Lanes 2-4 : IR Dye 680 Conjugated Goat anti Rabbit IgG (H+L) at 1/21250 dilution
Performed under reducing conditions.
Predicted band size: 199 kDa
Observed band size: 230 kDa why is the actual band size different from the predicted?
Ab32820 detects a band at approximately 230kDa. Other antibodies to this target detect a band in this region and it is understood that this band corresponds to the native Spt6 protein. -
Spt6 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Spt6 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab32820.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 230kDa: Spt6
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab32820 は 11 報の論文で使用されています。
- Bobkov GOM et al. Spt6 is a maintenance factor for centromeric CENP-A. Nat Commun 11:2919 (2020). PubMed: 32522980
- Obara EAA et al. SPT6-driven error-free DNA repair safeguards genomic stability of glioblastoma cancer stem-like cells. Nat Commun 11:4709 (2020). PubMed: 32948765
- Dembowski JA et al. Replication-Coupled Recruitment of Viral and Cellular Factors to Herpes Simplex Virus Type 1 Replication Forks for the Maintenance and Expression of Viral Genomes. PLoS Pathog 13:e1006166 (2017). PubMed: 28095497
- Soboleva TA et al. A new link between transcriptional initiation and pre-mRNA splicing: The RNA binding histone variant H2A.B. PLoS Genet 13:e1006633 (2017). ChIP ; Mouse . PubMed: 28234895
- Gérard A et al. The integrase cofactor LEDGF/p75 associates with Iws1 and Spt6 for postintegration silencing of HIV-1 gene expression in latently infected cells. Cell Host Microbe 17:107-17 (2015). PubMed: 25590759