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  1. Link

    spr-antibody-epr9290-ab157194.pdf

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Neuroscience Neurotransmission Nitric Oxide NOS
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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-SPR 抗体 [EPR9290] (ab157194)

  • Datasheet
Reviews (1) Submit a question References (3)

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-SPR antibody [EPR9290] (ab157194)
  • Western blot - Anti-SPR antibody [EPR9290] (ab157194)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPR antibody [EPR9290] (ab157194)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPR antibody [EPR9290] (ab157194)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPR antibody [EPR9290] (ab157194)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPR antibody [EPR9290] (ab157194)
  • Flow Cytometry - Anti-SPR antibody [EPR9290] (ab157194)
  • Immunocytochemistry/ Immunofluorescence - Anti-SPR antibody [EPR9290] (ab157194)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPR antibody [EPR9290] (ab157194)
  • Immunoprecipitation - Anti-SPR antibody [EPR9290] (ab157194)
  • Anti-SPR antibody [EPR9290] (ab157194)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9290] to SPR
  • Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt
  • Knockout validated
  • Reacts with: Human

こちらの製品もご検討ください

タンパク質
Product image
Recombinant Mouse SPR protein (His tag) (ab226880)
ノックアウト
Product image
Human SPR knockout HeLa cell line (ab264681)

関連製品

製品の概要

  • 製品名

    Anti-SPR antibody [EPR9290]
    SPR 一次抗体 製品一覧
  • 製品の詳細

    Rabbit monoclonal [EPR9290] to SPR
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: WB, IHC-P, ICC/IF, IP, Flow Cytmore details
  • 種交差性

    交差種: Human
  • 免疫原

    Synthetic peptide, corresponding to residues in Human SPR (UniProt: P35270).

  • ポジティブ・コントロール

    • WB: A549, MCF7, HepG2 and HeLa (ab150035) cell lysates. IHC-P: Human thyroid gland carcinoma tissue. ICC/IF: HeLa cells. Flow Cyt: MCF7 cells.
  • 特記事項

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at -20ºC.
  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • 精製度

    Protein A purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    EPR9290
  • アイソタイプ

    IgG
  • 研究分野

    • Neuroscience
    • Neurotransmission
    • Nitric Oxide
    • NOS
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Parkinson's disease
    • Other
    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Neuroscience
    • Neurotransmitter
    • Biogenic Amines
    • Other Amines
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism
    • Metabolism
    • Types of disease
    • Neurodegenerative disease

関連製品

  • Alternative Versions

    • Anti-SPR antibody [EPR9290] - BSA and Azide free (ab249302)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human SPR knockout HeLa cell line (ab264681)
  • KO cell lysates

    • Human SPR knockout HeLa cell lysate (ab258700)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • MCF7 whole cell lysate (ab29537)
    • HeLa whole cell lysate (ab29545)
    • A549 whole cell lysate (ab7910)
  • Recombinant Protein

    • Recombinant Mouse SPR protein (His tag) (ab226880)
  • Related Products

    • Recombinant Human SPR protein (ab86445)

アプリケーション

Our Abpromise guarantee covers the use of ab157194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能

    Catalyzes the final one or two reductions in tetra-hydrobiopterin biosynthesis to form 5,6,7,8-tetrahydrobiopterin.
  • 関連疾患

    Defects in SPR are the cause of dystonia DOPA-responsive due to sepiapterin reductase deficiency (DRDSPRD) [MIM:612716]. In the majority of cases, patients manifest progressive psychomotor retardation, dystonia and spasticity. Cognitive anomalies are also often present. The disease is due to severe dopamine and serotonin deficiencies in the central nervous system caused by a defect in BH4 synthesis. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures.
  • 配列類似性

    Belongs to the sepiapterin reductase family.
  • 翻訳後修飾

    In vitro phosphorylation of Ser-213 by CaMK2 does not change kinetic parameters.
  • 細胞内局在

    Cytoplasm.
  • Target information above from: UniProt accession P35270 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 6697 Human
    • Omim: 182125 Human
    • SwissProt: P35270 Human
    • Unigene: 301540 Human
    • 別名

      • OTTHUMP00000160199 antibody
      • SDR38C1 antibody
      • Sepiapterin reductase (7,8 dihydrobiopterin:NADP+ oxidoreductase) antibody
      • Sepiapterin reductase antibody
      • Short chain dehydrogenase/reductase family 38C, member 1 antibody
      • SPR antibody
      • SPRE_HUMAN antibody
      see all

    画像

    • Western blot - Anti-SPR antibody [EPR9290] (ab157194)
      Western blot - Anti-SPR antibody [EPR9290] (ab157194)
      All lanes : Anti-SPR antibody [EPR9290] (ab157194) at 1/1000 dilution

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : SPR knockout HeLa cell lysate
      Lane 3 : MCF7 cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 28 kDa
      Observed band size: 26 kDa
      why is the actual band size different from the predicted?



      Lanes 1-3: Merged signal (red and green). Green - ab157194 observed at 26 kDa. Red - loading control, ab8245 observed at 37 kDa.

      ab157194 Anti-SPR antibody [EPR9290] was shown to specifically react with SPR in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264681 (knockout cell lysate ab258700) was used. Wild-type and SPR knockout samples were subjected to SDS-PAGE. ab157194 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

       

    • Western blot - Anti-SPR antibody [EPR9290] (ab157194)
      Western blot - Anti-SPR antibody [EPR9290] (ab157194)
      All lanes : Anti-SPR antibody [EPR9290] (ab157194) at 1/1000 dilution

      Lane 1 : A549 cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 28 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPR antibody [EPR9290] (ab157194)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPR antibody [EPR9290] (ab157194)

      ab157194 showing +ve staining in Human normal pancreas.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPR antibody [EPR9290] (ab157194)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPR antibody [EPR9290] (ab157194)

      ab157194 showing +ve staining in Human heart.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPR antibody [EPR9290] (ab157194)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPR antibody [EPR9290] (ab157194)

      ab157194 showing +ve staining in Human endometrial carcinoma.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPR antibody [EPR9290] (ab157194)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPR antibody [EPR9290] (ab157194)

      ab157194 showing +ve staining in Human colonic adenocarcinoma.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Flow Cytometry - Anti-SPR antibody [EPR9290] (ab157194)
      Flow Cytometry - Anti-SPR antibody [EPR9290] (ab157194)
      Flow cytometic analysis of permeabilized MCF7 cells labeling TPST2 with ab157194 at 1/100 (red) or a rabbit IgG (negative) (green).
    • Immunocytochemistry/ Immunofluorescence - Anti-SPR antibody [EPR9290] (ab157194)
      Immunocytochemistry/ Immunofluorescence - Anti-SPR antibody [EPR9290] (ab157194)
      Immunofluorescent analysis of HeLa cells labeling TPST2 with ab157194 at 1/100 dilution.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPR antibody [EPR9290] (ab157194)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPR antibody [EPR9290] (ab157194)

      Immunohistochemical analysis of paraffin-eEmbedded Human thyroid gland carcinoma tissue labeling TPST2 with ab157194 at 1/100 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-SPR antibody [EPR9290] (ab157194)
      Immunoprecipitation - Anti-SPR antibody [EPR9290] (ab157194)
      Detection of TPST2 by Western Blot of Immunprecipitate. MCF7 cell lysate immunoprecipitated using ab157194 at 1/10 dilution.
    • Anti-SPR antibody [EPR9290] (ab157194)
      Anti-SPR antibody [EPR9290] (ab157194)

    プロトコール

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet
  • 参考文献 (3)

    ab157194 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab157194 は 3 報の論文で使用されています。

    • Wu Y  et al. Sepiapterin reductase promotes hepatocellular carcinoma progression via FoxO3a/Bim signaling in a nonenzymatic manner. Cell Death Dis 11:248 (2020). PubMed: 32312975
    • Mooney MR  et al. Anti-tumor effect of sulfasalazine in neuroblastoma. Biochem Pharmacol 162:237-249 (2019). PubMed: 30639262
    • Sallin O  et al. Semisynthetic biosensors for mapping cellular concentrations of nicotinamide adenine dinucleotides. Elife 7:N/A (2018). PubMed: 29809136

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Western blot abreview for Anti-SPR antibody [EPR9290]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rabbit Tissue lysate - whole (brain)
    Gel Running Conditions
    Non-reduced Denaturing
    Loading amount
    0.2 µg
    Treatment
    hypoxic 40 minutes at E25
    Specification
    brain
    Read More

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    投稿 Jan 25 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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