Anti-SPR 抗体 [EPR9290] (ab157194)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9290] to SPR
- Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt
- Knockout validated
- Reacts with: Human
製品の概要
-
製品名
Anti-SPR antibody [EPR9290]
SPR 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR9290] to SPR -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IF, IP, Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide, corresponding to residues in Human SPR (UniProt: P35270).
-
ポジティブ・コントロール
- WB: A549, MCF7, HepG2 and HeLa (ab150035) cell lysates. IHC-P: Human thyroid gland carcinoma tissue. ICC/IF: HeLa cells. Flow Cyt: MCF7 cells.
-
特記事項
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20ºC. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR9290 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
KO cell lines
-
KO cell lysates
-
Positive Controls
-
Recombinant Protein
-
Related Products
アプリケーション
Our Abpromise guarantee covers the use of ab157194 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | 1/1000 - 1/10000. Predicted molecular weight: 28 kDa. | |
IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
ICC/IF | 1/100 - 1/250. | |
IP | 1/10 - 1/100. | |
Flow Cyt | 1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
-
機能
Catalyzes the final one or two reductions in tetra-hydrobiopterin biosynthesis to form 5,6,7,8-tetrahydrobiopterin. -
関連疾患
Defects in SPR are the cause of dystonia DOPA-responsive due to sepiapterin reductase deficiency (DRDSPRD) [MIM:612716]. In the majority of cases, patients manifest progressive psychomotor retardation, dystonia and spasticity. Cognitive anomalies are also often present. The disease is due to severe dopamine and serotonin deficiencies in the central nervous system caused by a defect in BH4 synthesis. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. -
配列類似性
Belongs to the sepiapterin reductase family. -
翻訳後修飾
In vitro phosphorylation of Ser-213 by CaMK2 does not change kinetic parameters. -
細胞内局在
Cytoplasm. - Information by UniProt
-
参照データベース
- Entrez Gene: 6697 Human
- Omim: 182125 Human
- SwissProt: P35270 Human
- Unigene: 301540 Human
-
別名
- OTTHUMP00000160199 antibody
- SDR38C1 antibody
- Sepiapterin reductase (7,8 dihydrobiopterin:NADP+ oxidoreductase) antibody
see all
画像
-
All lanes : Anti-SPR antibody [EPR9290] (ab157194) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : SPR knockout HeLa cell lysate
Lane 3 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 28 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab157194 observed at 26 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab157194 Anti-SPR antibody [EPR9290] was shown to specifically react with SPR in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264681 (knockout cell lysate ab258700) was used. Wild-type and SPR knockout samples were subjected to SDS-PAGE. ab157194 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
-
All lanes : Anti-SPR antibody [EPR9290] (ab157194) at 1/1000 dilution
Lane 1 : A549 cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 28 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPR antibody [EPR9290] (ab157194)
ab157194 showing +ve staining in Human normal pancreas.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPR antibody [EPR9290] (ab157194)
ab157194 showing +ve staining in Human heart.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPR antibody [EPR9290] (ab157194)
ab157194 showing +ve staining in Human endometrial carcinoma.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPR antibody [EPR9290] (ab157194)
ab157194 showing +ve staining in Human colonic adenocarcinoma.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
Flow cytometic analysis of permeabilized MCF7 cells labeling TPST2 with ab157194 at 1/100 (red) or a rabbit IgG (negative) (green).
-
Immunofluorescent analysis of HeLa cells labeling TPST2 with ab157194 at 1/100 dilution.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPR antibody [EPR9290] (ab157194)
Immunohistochemical analysis of paraffin-eEmbedded Human thyroid gland carcinoma tissue labeling TPST2 with ab157194 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
Detection of TPST2 by Western Blot of Immunprecipitate. MCF7 cell lysate immunoprecipitated using ab157194 at 1/10 dilution.
プロトコール
データシートおよび資料
参考文献 (3)
ab157194 は 3 報の論文で使用されています。
- Wu Y et al. Sepiapterin reductase promotes hepatocellular carcinoma progression via FoxO3a/Bim signaling in a nonenzymatic manner. Cell Death Dis 11:248 (2020). PubMed: 32312975
- Mooney MR et al. Anti-tumor effect of sulfasalazine in neuroblastoma. Biochem Pharmacol 162:237-249 (2019). PubMed: 30639262
- Sallin O et al. Semisynthetic biosensors for mapping cellular concentrations of nicotinamide adenine dinucleotides. Elife 7:N/A (2018). PubMed: 29809136