Name: Anti-SPR antibody [EPR9290]
Brand: RabMAb
SKU: ab157194
Rating: 4 (1 reviews)

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Western blot - Anti-SPR antibody [EPR9290] (ab157194)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR9290] to SPR
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP
Knockout validated
Reacts with: Human

Carrier Free

製品名

Anti-SPR antibody [EPR9290]
SPR 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR9290] to SPR
由来種

Rabbit
アプリケーション

適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IPmore details
種交差性

交差種: Human
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- WB: A549, MCF7, HepG2 and HeLa (ab150035) cell lysates. IHC-P: Human thyroid gland carcinoma tissue. ICC/IF: HeLa cells. Flow Cyt (intra): MCF7 cells.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at -20ºC.
バッファー

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR9290
アイソタイプ

IgG
研究分野

Alternative Versions
- Anti-SPR antibody [EPR9290] - BSA and Azide free (ab249302)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
KO cell lines
- Human SPR knockout HeLa cell line (ab264681)
KO cell lysates
- Human SPR knockout HeLa cell lysate (ab258700)
Positive Controls
Recombinant Protein
- Recombinant Mouse SPR protein (His tag) (ab226880)
Related Products
- Recombinant Human SPR protein (ab86445)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab157194の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
Flow Cyt (Intra)		1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB	(1)	1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
IHC-P		1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF		1/100 - 1/250.
IP		1/10 - 1/100.

特記事項
Flow Cyt (Intra) 1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.

機能

Catalyzes the final one or two reductions in tetra-hydrobiopterin biosynthesis to form 5,6,7,8-tetrahydrobiopterin.
関連疾患

Defects in SPR are the cause of dystonia DOPA-responsive due to sepiapterin reductase deficiency (DRDSPRD) [MIM:612716]. In the majority of cases, patients manifest progressive psychomotor retardation, dystonia and spasticity. Cognitive anomalies are also often present. The disease is due to severe dopamine and serotonin deficiencies in the central nervous system caused by a defect in BH4 synthesis. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures.
配列類似性

Belongs to the sepiapterin reductase family.
翻訳後修飾

In vitro phosphorylation of Ser-213 by CaMK2 does not change kinetic parameters.
細胞内局在

Cytoplasm.
Target information above from: UniProt accession P35270 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 6697 Human
- Omim: 182125 Human
- SwissProt: P35270 Human
- Unigene: 301540 Human
別名
- OTTHUMP00000160199 antibody
- SDR38C1 antibody
- Sepiapterin reductase (7,8 dihydrobiopterin:NADP+ oxidoreductase) antibody
- Sepiapterin reductase antibody
- Short chain dehydrogenase/reductase family 38C, member 1 antibody
- SPR antibody
- SPRE_HUMAN antibody
see all

Western blot - Anti-SPR antibody [EPR9290] (ab157194)

All lanes : Anti-SPR antibody [EPR9290] (ab157194) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : SPR knockout HeLa cell lysate
Lane 3 : MCF7 cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 28 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?

Lanes 1-3: Merged signal (red and green). Green - ab157194 observed at 26 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab157194 Anti-SPR antibody [EPR9290] was shown to specifically react with SPR in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264681 (knockout cell lysate ab258700) was used. Wild-type and SPR knockout samples were subjected to SDS-PAGE. ab157194 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-SPR antibody [EPR9290] (ab157194)

All lanes : Anti-SPR antibody [EPR9290] (ab157194) at 1/1000 dilution

Lane 1 : A549 cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HeLa cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 28 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPR antibody [EPR9290] (ab157194)

ab157194 showing +ve staining in Human normal pancreas.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPR antibody [EPR9290] (ab157194)

ab157194 showing +ve staining in Human heart.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPR antibody [EPR9290] (ab157194)

ab157194 showing +ve staining in Human endometrial carcinoma.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPR antibody [EPR9290] (ab157194)

ab157194 showing +ve staining in Human colonic adenocarcinoma.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cytometry (Intracellular) - Anti-SPR antibody [EPR9290] (ab157194)

Flow cytometic analysis of permeabilized MCF7 cells labeling TPST2 with ab157194 at 1/100 (red) or a rabbit IgG (negative) (green).
Immunocytochemistry/ Immunofluorescence - Anti-SPR antibody [EPR9290] (ab157194)

Immunofluorescent analysis of HeLa cells labeling TPST2 with ab157194 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPR antibody [EPR9290] (ab157194)

Immunohistochemical analysis of paraffin-eEmbedded Human thyroid gland carcinoma tissue labeling TPST2 with ab157194 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunoprecipitation - Anti-SPR antibody [EPR9290] (ab157194)

Detection of TPST2 by Western Blot of Immunprecipitate. MCF7 cell lysate immunoprecipitated using ab157194 at 1/10 dilution.
Anti-SPR antibody [EPR9290] (ab157194)

Flow cytometry protocols
Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

Datasheet download

Download

ab157194 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab157194 は 3 報の論文で使用されています。

Wu Y et al. Sepiapterin reductase promotes hepatocellular carcinoma progression via FoxO3a/Bim signaling in a nonenzymatic manner. Cell Death Dis 11:248 (2020). PubMed: 32312975
Mooney MR et al. Anti-tumor effect of sulfasalazine in neuroblastoma. Biochem Pharmacol 162:237-249 (2019). PubMed: 30639262
Sallin O et al. Semisynthetic biosensors for mapping cellular concentrations of nicotinamide adenine dinucleotides. Elife 7:N/A (2018). PubMed: 29809136

レビューと Q&A

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Application

Western blot

Sample

Rabbit Tissue lysate - whole (brain)

Gel Running Conditions

Non-reduced Denaturing

Loading amount

0.2 µg

Treatment

hypoxic 40 minutes at E25

Specification

brain

Abcam user community

Verified customer

投稿 Jan 25 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Key features and details

Related conjugates and formulations

製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Isotype control

KO cell lines

KO cell lysates

Positive Controls

Recombinant Protein

Related Products

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

関連疾患

配列類似性

翻訳後修飾

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

Datasheet download

参考文献 (3)

レビューと Q&A

Western blot abreview for Anti-SPR antibody [EPR9290]