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Anti-SPINK1/P12 抗体 [SP166] - BSA and Azide free (ab271943)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPINK1/P12 antibody [SP166] - BSA and Azide free (ab271943)
  • Anti-SPINK1/P12 antibody [SP166] - BSA and Azide free (ab271943)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP166] to SPINK1/P12 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Human

製品の概要

  • 製品名

    Anti-SPINK1/P12 antibody [SP166] - BSA and Azide free
    SPINK1/P12 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [SP166] to SPINK1/P12 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant full length protein corresponding to Human SPINK1/P12 aa 1 to the C-terminus. Without signal peptide.
    Database link: P00995

  • ポジティブ･コントロール

    • Human prostate adenocarcinoma tissue; Human pancreas lysate

  • 特記事項

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    Cow: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product was previously labelled as SPINK1.

    ab271943 is the carrier-free version of ab183347. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • バッファー

    pH: 7.20
    Constituent: PBS

  • キャリア・フリー

    はい
  • Concentration information loading...

  • 精製度

    Protein A/G purified

  • 特記事項（精製）

    Purified from TCS by protein A/G.

  • ポリ/モノ

    モノクローナル

  • クローン名

    SP166

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab271943 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 8 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 8 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Serine protease inhibitor which exhibits anti-trypsin activity (PubMed:7142173). In the pancreas, protects against trypsin-catalyzed premature activation of zymogens.
    In the male reproductive tract, binds to sperm heads where it modulates sperm capacitance by inhibiting calcium uptake and nitrogen oxide (NO) production.

  • 関連疾患

    Pancreatitis, hereditary
    Tropical calcific pancreatitis

  • 配列類似性

    Contains 1 Kazal-like domain.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • ISK1_HUMAN antibody
      • Pancreatic secretory trypsin inhibitor antibody
      • Pancreatic serine protease inhibitor Kazal antibody
      • PCTT antibody
      • PSTI antibody
      • Serine protease inhibitor Kazal type 1 antibody
      • Serine protease inhibitor Kazal-type 1 antibody
      • Spink 3 antibody
      • SPINK1 antibody
      • Spink3 antibody
      • TaTI antibody
      • TCP antibody
      • Tumor associated trypsin inhibitor antibody
      • Tumor-associated trypsin inhibitor antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPINK1/P12 antibody [SP166] - BSA and Azide free (ab271943)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPINK1/P12 antibody [SP166] - BSA and Azide free (ab271943)

      Immunohistochemical analysis of paraffin embedded Human prostate adenocarcinoma tissue labeling SPINK1/P12 with ab183347 at 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183347).
    • Anti-SPINK1/P12 antibody [SP166] - BSA and Azide free (ab271943)
      Anti-SPINK1/P12 antibody [SP166] - BSA and Azide free (ab271943)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab271943 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab271943 は論文での使用が確認できていません。

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com