Anti-Sphingomyelin Synthase 2 抗体 (ab272570)
Key features and details
- Rabbit polyclonal to Sphingomyelin Synthase 2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Sphingomyelin Synthase 2 antibody
Sphingomyelin Synthase 2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Sphingomyelin Synthase 2 -
由来種
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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免疫原
Recombinant fragment corresponding to Human Sphingomyelin Synthase 2 aa 1-100.
Database link: Q8NHU3 -
ポジティブ･コントロール
- WB: RT4, U-251 MG sp. IHC-P: Human lung tissue, Fallopian tube tissue, small intestine tissue, gallbladder tissue, skeletal muscle tissue.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272570 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| IHC-P |
Human
|
| WB |
Human
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
|
|
| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
|
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Bidirectional lipid cholinephosphotransferase capable of converting phosphatidylcholine (PC) and ceramide to sphingomyelin (SM) and diacylglycerol (DAG) and vice versa. Direction is dependent on the relative concentrations of DAG and ceramide as phosphocholine acceptors. Directly and specifically recognizes the choline head group on the substrate. Also requires two fatty chains on the choline-P donor molecule in order to be recognized efficiently as a substrate. Does not function strictly as a SM synthase. Required for cell growth. -
組織特異性
Brain, heart, kidney, liver, muscle and stomach. -
配列類似性
Belongs to the sphingomyelin synthase family. -
翻訳後修飾
Palmitoylated on Cys-331, Cys-332, Cys-343 and Cys-348; which plays an important role in plasma membrane localization. -
細胞内局在
Cell membrane. Golgi apparatus membrane. Predominantly plasma membrane, some in Golgi apparatus. - Information by UniProt
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参照データベース
- Entrez Gene: 166929 Human
- Omim: 611574 Human
- SwissProt: Q8NHU3 Human
- Unigene: 595423 Human
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別名
- MGC26963 antibody
- Phosphatidylcholine:ceramide cholinephosphotransferase 2 antibody
- SGMS 2 antibody
see all
画像
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Western blot - Anti-Sphingomyelin Synthase 2 antibody (ab272570)Lanes 2-3 : Anti-Sphingomyelin Synthase 2 antibody (ab272570) at 0.4 µg/ml
Lane 1 : Molecular weight ladder
Lane 2 : RT4
Lane 3 : U-251 MG sp -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sphingomyelin Synthase 2 antibody (ab272570)Paraffin-embedded formalin-fixed Human lung tissue stained for Sphingomyelin Synthase 2 using ab272570 at 1/500 in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sphingomyelin Synthase 2 antibody (ab272570)Paraffin-embedded formalin-fixed Human Fallopian tube tissue stained for Sphingomyelin Synthase 2 using ab272570 at 1/500 in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sphingomyelin Synthase 2 antibody (ab272570)Paraffin-embedded formalin-fixed Human small intestine tissue stained for Sphingomyelin Synthase 2 using ab272570 at 1/500 in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sphingomyelin Synthase 2 antibody (ab272570)Paraffin-embedded formalin-fixed Human gallbladder tissue stained for Sphingomyelin Synthase 2 using ab272570 at 1/500 in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sphingomyelin Synthase 2 antibody (ab272570)Paraffin-embedded formalin-fixed Human skeletal muscle tissue stained for Sphingomyelin Synthase 2 using ab272570 1/500 in immunohistochemical analysis.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab272570 は論文での使用が確認できていません。
