Sphingomyelin Assay Kit (Fluorometric) (ab138877)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell culture extracts, Whole Blood
- Sensitivity: 1 µM
製品の概要
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製品名
Sphingomyelin Assay Kit (Fluorometric)
Sphingomyelin キット 製品一覧 -
検出方法
Fluorescent -
サンプルの種類
Cell culture extracts, Whole Blood -
アッセイタイプ
Quantitative -
検出感度
1 µM -
種交差性
交差種: Mammals, Other species -
製品の概要
Abcam's Sphingomyelin Assay Kit (Fluorometric) (ab138877) provides the most sensitive method for detecting neutral SM activity or screening SM inhibitors. The kit uses AbRed Indicator as a fluorogenic probe to indirectly quantify the phosphocholine produced from the hydrolysis of sphingomyelin (SM) by sphingomyelinase (SMase). It can be used for measuring the amount of SM in blood, cell extracts or other solutions. The fluorescence intensity of AbRed Indicator is proportional to the formation of phosphocholine, therefore to the amount of SM. AbRed Indicator enables the assay readable by either a fluorescence reader or an absorbance reader.
The kit is an optimized “mix and read” assay that can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step.
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特記事項
Sphingomyelin (SM) is largely found in the exoplasmic leaflet of the cell membrane, primarily in nervous tissue. It plays an important role in signal transduction. Sphingomyelin accumulates abnormally in Niemann-Pick disease and Abetalipoproteinemia.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests AbRed Indicator 1 vial Assay Buffer 1 x 5ml DMSO 1 x 200µl Enzyme Mix 1 vial SMase Reaction Buffer 1 x 20ml 50mM Sphingomyelin standard 1 x 20µl Sphingomyelinase 1 vial -
研究分野
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab138877 は 6 報の論文で使用されています。
- Lee TY et al. Platelet autophagic machinery involved in thrombosis through a novel linkage of AMPK-MTOR to sphingolipid metabolism. Autophagy 17:4141-4158 (2021). PubMed: 33749503
- Kreutzberger AJB et al. Distinct insulin granule subpopulations implicated in the secretory pathology of diabetes types 1 and 2. Elife 9:N/A (2020). PubMed: 33164744
- Im EJ et al. Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A. Nat Commun 10:1387 (2019). PubMed: 30918259
- Pang Y et al. Crystal structure of a cytocidal protein from lamprey and its mechanism of action in the selective killing of cancer cells. Cell Commun Signal 17:54 (2019). PubMed: 31133022
- Faict S et al. The Transfer of Sphingomyelinase Contributes to Drug Resistance in Multiple Myeloma. Cancers (Basel) 11:N/A (2019). PubMed: 31756922
- Aligeti M et al. Cooperation between the Hepatitis C Virus p7 and NS5B Proteins Enhances Virion Infectivity. J Virol 89:11523-33 (2015). PubMed: 26355084