Anti-SPC25 抗体 (ab20679)
Key features and details
- Rabbit polyclonal to SPC25
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-SPC25 antibody -
製品の詳細
Rabbit polyclonal to SPC25 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human Spc25.
Immunogen の所有権に関して (Peptide available as ab23408.) -
ポジティブ･コントロール
- WB: Human pancreas and adrenal gland lysates
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab20679の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 26 kDa).
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| 特記事項 |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 26 kDa). |
ターゲット情報
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機能
Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. -
配列類似性
Belongs to the SPC25 family. -
細胞内局在
Nucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore. - Information by UniProt
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参照データベース
- Entrez Gene: 57405 Human
- Omim: 609395 Human
- SwissProt: Q9HBM1 Human
- Unigene: 421956 Human
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別名
- AD024 antibody
- hSpc25 antibody
- Kinetochore protein Spc25 antibody
see all
画像
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Western blot - Anti-SPC25 antibody (ab20679)All lanes : Anti-SPC25 antibody (ab20679) at 1 µg/ml
Lane 1 : Human Pancreas lysate
Lane 2 : Human Adrenal gland lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution
Predicted band size: 26 kDa
Observed band size: 31 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutesPrimary Conc: 1 µg/ml
Blocking buffer: 2% BSA
Gel type: MES
データシートおよび資料
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Datasheet download
参考文献 (4)
ab20679 は 4 報の論文で使用されています。
- Chen YJ et al. A Combined Systemic Strategy for Overcoming Cisplatin Resistance in Head and Neck Cancer: From Target Identification to Drug Discovery. Cancers (Basel) 12:N/A (2020). PubMed: 33238517
- Chen J et al. SPC25 upregulation increases cancer stem cell properties in non-small cell lung adenocarcinoma cells and independently predicts poor survival. Biomed Pharmacother 100:233-239 (2018). WB . PubMed: 29432994
- Villegas E et al. Plk2 regulates mitotic spindle orientation and mammary gland development. Development 141:1562-71 (2014). WB . PubMed: 24598160
- Sun SC et al. Perturbation of Spc25 expression affects meiotic spindle organization, chromosome alignment and spindle assembly checkpoint in mouse oocytes. Cell Cycle 9:4552-9 (2010). ICC/IF ; Mouse . PubMed: 21084868
