Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (embryo 9 weeks of gestation)
Permeabilization
Yes - Triton
Specification
embryo 9 weeks of gestation
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 21°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.1%Triton/0.25% Gelatin 1x PBS
Dilution
1/400
Secondary antibody
Name
Non-Abcam antibody was used: JacksonIR Donkey anti-Rabbit, mcr, #711165152
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
Additional data
Additional Notes
Fixation in 4% Paraformaldehyde for one day, cryoprotected in sucrose 10%, Cryostat sections
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Alain Chedotal
Verified customer
投稿 Jan 20 2021