Application
IHC - Wholemount
Sample
Mouse Embryo (E12.5 whole embryo)
Specification
E12.5 whole embryo
Other product details
Incubation time
99 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: PBS 1x + 0.5% Gelatin + 0.5% triton
Dilution
1/500
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
Incubated for 7 days at 37°C in primary antibody
1 day in the secondary at 37°C
then cleared using iDisco+ protocol
Imaged with light sheet microscopy
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Alain Chedotal
Verified customer
投稿 Nov 23 2020