Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Dog Tissue sections (Optic nerve)
Antigen retrieval step
None
Permeabilization
Yes - TritonX-100 + Tween20
Specification
Optic nerve
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.01M PBS + 0.05% Tween-20
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Additional data
Additional Notes
4% PFA was used for fixation. The image was acquired by epifluorescence microscopy. SOX9 (magenta).
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投稿 Jan 16 2020