Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Monodelphis domestica Tissue sections (spinal cord)
Antigen retrieval step
None
Permeabilization
Yes - 0.3% Triton X-100 in PBS for 1 hour
Specification
spinal cord
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Fixative
Paraformaldehyde
Other product details
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1%BSA+1%goat serum+0,1% Tween 20 in PBS
Dilution
1/500
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: goat anti-rabbit cross-adsorbed Alexa Fluor Plus 5
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Additional data
Additional Notes
works on spinal cord from neonatal P6 and P18 opossums (Monodelphis domestica)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Jelena Ban
Verified customer
投稿 Nov 08 2018