Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (mouse intestinal organoids)
Specification
mouse intestinal organoids
Blocking step
Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Fixative
Paraformaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: goat serum + 0.01% TX-100
Dilution
1/50
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: anti-Rabbit IgG (H+L) (Thermo Fisher, A-21245)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 647
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 647
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Sylke Hoehnel
Verified customer
投稿 Jun 20 2017