Anti-SOX8 抗体 (ab104245)
Key features and details
- Rabbit polyclonal to SOX8
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-SOX8 antibody
SOX8 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SOX8 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Dog, Macaque monkey, Gorilla
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免疫原
Synthetic peptide corresponding to Human SOX8 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab125935) -
ポジティブ･コントロール
- This antibody gave a positive signal in both Human Testis and Human Colon tissue lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab104245の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 47 kDa).
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| 特記事項 |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 47 kDa). |
ターゲット情報
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機能
May play a role in central nervous system, limb and facial development. May be involved in male sex determination. Binds the consensus motif 5'-[AT][AT]CAA[AT]G-3'. -
配列類似性
Contains 1 HMG box DNA-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 30812 Human
- Entrez Gene: 20681 Mouse
- Entrez Gene: 302993 Rat
- Omim: 605923 Human
- SwissProt: P57073 Human
- SwissProt: Q04886 Mouse
- Unigene: 243678 Human
- Unigene: 258220 Mouse
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別名
- MGC24837 antibody
- SOX 8 antibody
- Sox8 antibody
see all
画像
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Western blot - Anti-SOX8 antibody (ab104245)All lanes : Anti-SOX8 antibody (ab104245) at 1 µg/ml
Lane 1 : Human testis tissue lysate - total protein (ab30257)
Lane 2 : Human colon tissue lysate - total protein (ab30051)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutes
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab104245 は 2 報の論文で使用されています。
- Kimura S et al. Sox8 is essential for M cell maturation to accelerate IgA response at the early stage after weaning in mice. J Exp Med 216:831-846 (2019). PubMed: 30877171
- Gryder BE et al. Histone hyperacetylation disrupts core gene regulatory architecture in rhabdomyosarcoma. Nat Genet 51:1714-1722 (2019). PubMed: 31784732