Application
Western blot
Sample
Rat Cell lysate - whole cell (Hippocampal)
Gel Running Conditions
Reduced Denaturing (4-15% gradient)
Loading amount
40 µg
Specification
Hippocampal
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS-Tween 0.1%
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Invitrogen, Donkey anti-Rabbit IgG (H+L) Highly Cr
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Detection
Detection method
Fluorescence (Odissey)
Exposure
7 minute(s) and 0 second(s)
Bands
Specific: 40 kDa
Additional data
Additional Notes
Predicted band size: 34 kDa Observed band size: 40 kDa
Abcam response
We would like to thank the reviewer for submitting this AbReview for ab92494. We are pleased that this antibody worked well for you on rat samples, a species that we have not tested yet. We encourage all researchers to contact us should they wish to test any of our products in untested species or applications, as these may qualify for our AbTrial testing program.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Feb 26 2020