Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Neuromesodermal Progenitors)
Permeabilization
Yes - 0.5% Triton X-100
Specification
Neuromesodermal Progenitors
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking buffer
Dilution
1/200
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Additional data
Additional Notes
10% FCS, 0.1% BSA in PBS used for blocking
The IF was performed in Neuromesodermal Progenitors (NMPs) which express high levels of Sox2
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MISS. Antigoni Gogolou
Verified customer
投稿 Nov 13 2018