Application
Western blot
Sample
Xenopus laevis Recombinant protein (Clone expressed in Promega's TnT® SP6 High-Yield)
Gel Running Conditions
Non-reduced Denaturing (BioRad TGX Fast Cast 12%)
Loading amount
10 µg
Specification
Clone expressed in Promega's TnT® SP6 High-Yield
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk in PBS-Tween
Dilution
1/1000
Secondary antibody
Dilution
1/2500
Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked Antibody #7074
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Negative control
No-template IVT mix (lane 1), and sox21-FLAG IVT (lane 3).
Exposure
30 second(s)
Bands
Specific: 37 kDa
Positive control
anti-HA-HRP blot (blot C.)
Additional data
Additional Notes
Loading amount set to default 10ug. However, given these antibodies were tested qualitatively, the samples were not quantified.
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投稿 May 24 2018