Application
Immunocytochemistry/ Immunofluorescence
Sample
Zebrafish Cell (whole embryo 3.5-4.5 hours past fertilization)
Permeabilization
Yes - -20°C MeOH storage over night
Specification
whole embryo 3.5-4.5 hours past fertilization
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 23°C
Fixative
Paraformaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: PBS + 0.5% BSA, 0.25% Triton X-100
Dilution
1/100
Secondary antibody
Dilution
1/400
Name
Non-Abcam antibody was used: Donkey anti-rabbit IgG Secondary
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Additional data
Additional Notes
Only unspecific background signal is visible across the embryo.
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jul 14 2021