Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (E13 spinal cord)
Specification
E13 spinal cord
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Other product details
Dilution
1/3000
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Secondary antibody
Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200
Additional data
Additional Notes
Localisation of nuclear positivity appears to conform to that expected. Submitted image of developing spinal cord ( T/S) shows clear nuclear positivity in the ventricular region and also in a subset of cells clustered around ganglion cells in a dorsal root ganglion.
Interestingly, optimal dilution factors vary between mouse and quail, in my hands.
Interestingly, optimal dilution factors vary between mouse and quail, in my hands.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
投稿 Sep 08 2010