Anti-SOX2 抗体 (ab97959)
Key features and details
- Rabbit polyclonal to SOX2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-SOX2 antibody
SOX2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SOX2 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Sheep, Horse, Chicken, Cow, Pig, Xenopus laevis, Zebrafish, Quail, Rhesus monkey, Rainbow trout, Spotted catshark, Thornback ray -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- ICC/IF: NCCIT and NIH/3T3 cells. Dissociated induced pluripotent stem cells from mouse embryonic fibroblasts. Mouse embryonic stem cells. WB: NCCIT, IOUD2, HUES7, F9 and MCF7 whole cell lysate. IHC: Human brain glioma.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1 mg/ml may have BSA added as a stabilizing agent. If you would like information about the formulation of a specific lot, please contact our Scientific Support Team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97959の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | (24) |
Use a concentration of 1 mg/ml.
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WB | (14) |
Use a concentration of 1 µg/ml. Detects a band of approximately 34,40 kDa (predicted molecular weight: 34 kDa).
Recombinant Human SOX2 protein (ab80520) can be used as a positive control in WB. |
ICC/IF | (12) |
Use a concentration of 1 µg/ml.
We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody. |
特記事項 |
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IHC-P
Use a concentration of 1 mg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 34,40 kDa (predicted molecular weight: 34 kDa). Recombinant Human SOX2 protein (ab80520) can be used as a positive control in WB. |
ICC/IF
Use a concentration of 1 µg/ml. We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody. |
ターゲット情報
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機能
Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. -
関連疾患
Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula. -
配列類似性
Contains 1 HMG box DNA-binding domain. -
翻訳後修飾
Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 396105 Chicken
- Entrez Gene: 784383 Cow
- Entrez Gene: 6657 Human
- Entrez Gene: 20674 Mouse
- Entrez Gene: 407739 Pig
- Entrez Gene: 499593 Rat
- Entrez Gene: 398000 Xenopus laevis
- Entrez Gene: 378723 Zebrafish
see all -
別名
- ANOP3 antibody
- cb236 antibody
- Delta EF2a antibody
see all
画像
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ab97959 staining SOX2 in mES cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab97959 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 100% methanol (5 min).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
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IHC image of SOX2 staining in Human brain glioma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab97959, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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ab97959 staining SOX2 in primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab97959 at 0.1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Cell line: NCCIT (human pluripotent embryonal carcinoma)
Target AbID: Ab97959 anti-Sox2, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody was used.
Counterstain AbID: Ab7291 anti-Tubulin (Rabbit mAb), 97959
Fixative: 4% PFA
Permeabilisation: 0.1% Triton-X
Nuclear counter stain: DAPI
Comments: Confocal image showing negative staining on NCCIT cells
Target primary antibody dilution: 1:500
Target secondary antibody dilution: 1:1000 (2ug/mL)
Counterstain primary antibody dilution: 1:1000 (1ug/mL)
Counterstain secondary antibody dilution: 1:1000 (2ug/mL)
Negative control 1 primary antibody dilution: 1:500 (Ab97959)
Negative control 1 secondary antibody dilution: 1:1000 (2ug/mL) (Ab150120)
Negative control 2 primary antibody dilution: 1:1000 (1ug/mL) (Ab7291)
Negative control 2 secondary antibody dilution: 1:1000 (2ug/mL) (Ab150077)
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All lanes : Anti-SOX2 antibody (ab97959) at 1 µg
Lane 1 : NCCIT (Human embryonic carcinoma cell line) Whole Cell Lysate
Lane 2 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 4 : C6 (Rat glial tumor cell line) Whole Cell Lysate
Lane 5 : Hippocampus (Rat) Tissue Lysate
Lane 6 : Spinal Cord (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Blots were developed with goat anti-rabbit IgG (H+L) and goat anti-mouse IgG (H+L) secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?Green signal from target - ab97959 observed at 40 kDa
Red signal from loading control ab9484 (GAPDH) observed at 37 kDa -
Cell line: NIH/3T3 (mouse embryonic fibroblast cell line)
Target AbID: Ab97959 anti-Sox2, used Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody
Counterstain AbID: Ab7291 anti-Tubulin (Rabbit mAb), Ab150120 AlexaFluor®594 Goat anti-Mouse secondary
Fixative: 4% PFA
Permeabilisation: 0.1% Triton-X
Nuclear counter stain: DAPI
Comments: Confocal image showing negative staining on NIH/3T3 cells
Target primary antibody dilution: 1:500
Target secondary antibody dilution: 1:1000 (2ug/mL)
Counterstain primary antibody dilution: 1:1000 (1ug/mL)
Counterstain secondary antibody dilution: 1:1000 (2ug/mL)
Negative control 1 primary antibody dilution: 1:500 (Ab97959)
Negative control 1 secondary antibody dilution: 1:1000 (2ug/mL) (Ab150120)
Negative control 2 primary antibody dilution: 1:1000 (1ug/mL) (Ab7291)
Negative control 2 secondary antibody dilution: 1:1000 (2ug/mL) (Ab150077)
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ICC/IF analysis of dissociated induced pluripotent stem cells from mouse embryonic fibroblasts stained for SOX-2 (Red) using ab97959. TOPRO (purple). Scale bar=100 μm
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All lanes : Anti-SOX2 antibody (ab97959) at 1 µg/ml
Lane 1 : IOUD2 (Mouse embryonic stem cell) Whole Cell Lysate
Lane 2 : HUES7 (Human embryonic stem cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
Additional bands at: 37 kDa, 39 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (656)
ab97959 は 656 報の論文で使用されています。
- Shi CJ et al. TGFβR-1/ALK5 inhibitor RepSox induces enteric glia-to-neuron transition and influences gastrointestinal mobility in adult mice. Acta Pharmacol Sin 44:92-104 (2023). PubMed: 35794374
- Espinosa-Medina I et al. TEMPO enables sequential genetic labeling and manipulation of vertebrate cell lineages. Neuron 111:345-361.e10 (2023). PubMed: 36417906
- Lee J et al. Production of large, defined genome modifications in rats by targeting rat embryonic stem cells. Stem Cell Reports 18:394-409 (2023). PubMed: 36525967
- Ohishi H et al. STREAMING-tag system reveals spatiotemporal relationships between transcriptional regulatory factors and transcriptional activity. Nat Commun 13:7672 (2022). PubMed: 36539402
- Xu X et al. Folate regulates RNA m5C modification and translation in neural stem cells. BMC Biol 20:261 (2022). PubMed: 36424632