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  1. Link

    sostdc1-antibody-ab99340.pdf

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Signal Transduction Growth Factors/Hormones TGF
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Anti-SOSTDC1 抗体 (ab99340)

  • Datasheet
Reviews (1)Q&A (5)References (9)

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Abpromise

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOSTDC1 antibody (ab99340)
  • Western blot - Anti-SOSTDC1 antibody (ab99340)
  • Immunocytochemistry/ Immunofluorescence - Anti-SOSTDC1 antibody (ab99340)

Key features and details

  • Rabbit polyclonal to SOSTDC1
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

こちらの製品もご検討ください

タンパク質
Product image
Recombinant Human SOSTDC1 protein (denatured) (ab139616)
二次抗体
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
一次抗体
Product image
Anti-BMP2 antibody [EPR20807] (ab214821)

関連製品

製品の概要

  • 製品名

    Anti-SOSTDC1 antibody
  • 製品の詳細

    Rabbit polyclonal to SOSTDC1
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性

    交差種: Mouse, Rat, Human
    交差が予測される動物種: Macaque monkey, Orangutan
  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ・コントロール

    • This antibody gave a positive signal in Human, Mouse and Rat Kidney tissue lysates. It also gave a positive signal in FFPE human normal lung tissue sections
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Signal Transduction
    • Growth Factors/Hormones
    • TGF
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • Structures
    • Bone

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human SOSTDC1 protein (denatured) (ab139616)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab99340の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
Use a concentration of 5 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 23 kDa).
IHC-P (1)
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
特記事項
ICC/IF
Use a concentration of 5 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 23 kDa).
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    May be involved in the onset of endometrial receptivity for implantation/sensitization for the decidual cell reaction Enhances Wnt signaling and inhibits TGF-beta signaling (By similarity). Directly antagonizes activity of BMP2, BMP4, BMP6 and BMP7 in a dose-dependent manner.
  • 組織特異性

    Highly expressed in kidney and weakly in lung.
  • 配列類似性

    Belongs to the sclerostin family.
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
  • 細胞内局在

    Secreted.
  • Target information above from: UniProt accession Q6X4U4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 25928 Human
    • Entrez Gene: 66042 Mouse
    • Entrez Gene: 266803 Rat
    • Omim: 609675 Human
    • SwissProt: Q6X4U4 Human
    • SwissProt: Q9CQN4 Mouse
    • SwissProt: Q642G2 Rat
    • Unigene: 648106 Human
    • Unigene: 43375 Mouse
    • Unigene: 2415 Rat
    see all
  • 別名

    • CDA019 antibody
    • DKFZp564D206 antibody
    • Ectodermal BMP inhibitor antibody
    • Ectodin antibody
    • sclerostin domain containing 1 antibody
    • Sclerostin domain containing protein 1 antibody
    • Sclerostin domain-containing protein 1 antibody
    • SOSD1_HUMAN antibody
    • Sostdc1 antibody
    • USAG-1 antibody
    • USAG1 antibody
    • Uterine sensitization associated gene 1 protein antibody
    • Uterine sensitization-associated gene 1 protein antibody
    • Wise antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOSTDC1 antibody (ab99340)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOSTDC1 antibody (ab99340)
    IHC image of SOSTDC1 staining in human normal lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab99340, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Western blot - Anti-SOSTDC1 antibody (ab99340)
    Western blot - Anti-SOSTDC1 antibody (ab99340)
    All lanes : Anti-SOSTDC1 antibody (ab99340) at 1 µg/ml

    Lane 1 : Human kidney tissue lysate - total protein (ab30203)
    Lane 2 : Kidney (Mouse) Tissue Lysate
    Lane 3 : Kidney (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 23 kDa
    Observed band size: 28 kDa why is the actual band size different from the predicted?


    Exposure time: 20 minutes


    SOSTDC1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
  • Immunocytochemistry/ Immunofluorescence - Anti-SOSTDC1 antibody (ab99340)
    Immunocytochemistry/ Immunofluorescence - Anti-SOSTDC1 antibody (ab99340)
    ICC/IF image of ab99340 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab99340, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) HeLa cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa and HepG2 cells at 5µg/ml.

プロトコール

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • Datasheet download

    Download

参考文献 (9)

ab99340 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab99340 は 9 報の論文で使用されています。

  • Lu L  et al. Febuxostat inhibits TGF-ß1-induced epithelial-mesenchymal transition via downregulation of USAG-1 expression in Madin-Darby canine kidney cells in vitro. Mol Med Rep 19:1694-1704 (2019). PubMed: 30628645
  • Gao B  et al. Macrophage-lineage TRAP+ cells recruit periosteum-derived cells for periosteal osteogenesis and regeneration. J Clin Invest 129:2578-2594 (2019). PubMed: 30946695
  • Valensi M  et al. Sostdc1 is expressed in all major compartments of developing and adult mammalian eyes. Graefes Arch Clin Exp Ophthalmol 257:2401-2427 (2019). PubMed: 31529323
  • Barbe MF  et al. Prolonged high force high repetition pulling induces osteocyte apoptosis and trabecular bone loss in distal radius, while low force high repetition pulling induces bone anabolism. Bone 110:267-283 (2018). IHC-P . PubMed: 29476978
  • Chen G  et al. SOSTDC1 inhibits bone metastasis in non-small cell lung cancer and may serve as a clinical therapeutic target. Int J Mol Med 42:3424-3436 (2018). PubMed: 30320379
  • Prochazkova M  et al. FGF signaling refines Wnt gradients to regulate the patterning of taste papillae. Development 144:2212-2221 (2017). PubMed: 28506989
  • Zhang Y  et al. Role of artesunate in TGF-ß1-induced renal tubular epithelial-mesenchymal transdifferentiation in NRK-52E cells. Mol Med Rep 16:8891-8899 (2017). PubMed: 28990102
  • Bansho Y  et al. Identification and characterization of secreted factors that are upregulated during somatic cell reprogramming. FEBS Lett 591:1584-1600 (2017). PubMed: 28471520
  • Yuan S  et al. Post-transcriptional Regulation of Keratinocyte Progenitor Cell Expansion, Differentiation and Hair Follicle Regression by miR-22. PLoS Genet 11:e1005253 (2015). PubMed: 26020521

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1-6 of 6 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SOSTDC1 antibody

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (pancreas)
Specification
pancreas
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
Yes - tritonX100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Nov 05 2012

Question

Hola ,
Sí yo creo que era lo que decías del tamaño del archivo. He hecho el montaje que me indicaste y puesto ahora la foto de una islet de pancreas y el control del secundario ( sin anticuerpo primario) que sale limpio.Todo correcto ahora?
Puedo hacer el nuevo pedido?
Muchas gracias por tu ayuda

Read More

Abcam community

Verified customer

Asked on Nov 05 2012

Answer

Gracias por enviarnos el Abreview.

Esta mañana lo he publicado en nuestra página web, con las imágenes incluidas. Ha quedado muy bien el montaje, gracias por molestarte.

El código puedes utilizarlo ya. Realiza tu pedido preferiblemente por mail, a mailto:orders@abcam.com indicando el código de descuento valido por un nuevo producto.

Si tienes cualquier otra consulta, no dudes en volverme a contactar.

Que pases una buena semana.

Read More

Abcam Scientific Support

Answered on Nov 05 2012

Question

Muchas gracias, muy amable ;)
a ver si hay suerte!

Read More

Abcam community

Verified customer

Asked on Jun 21 2012

Answer

¡Gracias a ti!

No olvides una vez enviados tus resultados a nuestra web, hacer el nuevo pedido indicando el código promocional para obtener un 100% de descuento.

Si necesitas mas información no dudes en contactarnos, por mail, o por teléfono.

Read More

Abcam Scientific Support

Answered on Jun 21 2012

Question

Hola,
Me gustaría probar el anticuerpo que he escritomás abajopara histología. Es válido para el descuento?

Read More

Abcam community

Verified customer

Asked on Jun 19 2012

Answer

Gracias por tu respuesta.

Sí que sería válido para testarlo en immunohistoquímica. Para poder mandarte el código es necesario indicar también si las secciones a teñir están congeladas o embebidas en parafina.

Una vez me lo indiques te envío el código para que puedas testarlo y mandarnos tu publicación.

Muchas gracias.

Read More

Abcam Scientific Support

Answered on Jun 19 2012

Question

Yes, I would like to use the antibody for an untestedtechnique. Would you mind please sending methe address of the Scientific Support person I should contact to?Thank you for your help in advance

Read More

Abcam community

Verified customer

Asked on Jun 18 2012

Answer

Thank you for your interest in our Abreview service. Let me explain how it works:

If you want to test any of the antibodies in an application / species not yet tested, follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test a specific antibody in a new species/technique.

I will then send a discount code. This code must be issued before purchasing the antibody so please wait for my reply before ordering.

2. Purchase the antibody either by phone, fax, or online (www.abcam.com).

3. Test it in the new species / application.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!).

To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Jun 18 2012

Question

Muchas gracias . Tiene que ser el mismo anticuerpo o puede ser otro?

Por cierto, vosotros no tendreis por casualidad un mapa de las rutas del metabolismo de estos de propaganda?
muchas gracias

Read More

Abcam community

Verified customer

Asked on Nov 14 2012

Answer

Puede ser cualquier otro anticuerpo de nuestro catálogo.

Te mando el link a nuestro catálogo de posters de rutas. Puedes descargártelo e imprimirlo, o si prefieres te lo podemos mandar por correo postal tamaño poster. Para ello mándame la dirección postal y cuál de ellos te interesa, y procedo al envío.

https://www.abcam.com/index.html?pageconfig=resource&rid=11652

¡Que tengas un buen día!

Read More

Abcam Scientific Support

Answered on Nov 14 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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  • 感染症
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