Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (e14.5 brain)
Specification
e14.5 brain
Fixative
Paraformaldehyde
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
Dilution
1/100
Incubation time
12 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Molecular probes
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Additional data
Additional Notes
Citric acid was used during antigen retrieval step
Abcam response
Thankyou for the Abreview, which confirms that this antibody is not suitable for IHC-Fr following paraformaldehyde fixation in mouse at the dilution used. However, the antibody is able to detect recombinant Sonic Hedgehog using Western Blotting.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Dean Griffiths
Verified customer
投稿 Jan 04 2007