Application
IHC - Wholemount
Sample
Leucoraja erinacea Embryo (Whole embryo)
Specification
Whole embryo
Other product details
Incubation time
48 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 10% fetal calf serum in 1X PBST
Dilution
1/200
Secondary antibody
Name
Non-Abcam antibody was used: Jackson Immunoresearch goat anti-Rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/600
Additional data
Additional Notes
Specimen was fixed overnight in 4% PFA (Electron Microscopy Sciences) at 4 degrees Celsius, rinsed in 1X PBS, and stored in 1X PBS at 4 degrees Celsius prior to IHC.
Abcam response
Thank you for your valuable feedback. To date ab19897 has not been tested in Leucoraja erinacea. It appears that under these experimental conditions, the antibody does not cross-react with this species. We welcome further customer feedback- for more information, please contact scientific support.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Andrew Gillis
Verified customer
投稿 Jul 25 2013