Anti-Sonic Hedgehog 抗体 (ab19897)
Key features and details
- Rabbit polyclonal to Sonic Hedgehog
- Suitable for: Sandwich ELISA, WB
- Reacts with: Mouse
- Isotype: IgG
製品の概要
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製品名
Anti-Sonic Hedgehog antibody
Sonic Hedgehog 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Sonic Hedgehog -
由来種
Rabbit -
アプリケーション
適用あり: Sandwich ELISA, WBmore details
適用なし: IHC-Fr -
種交差性
交差種: Mouse
交差が予測される動物種: Rat, Chicken, Cow, Human, Xenopus laevis, Zebrafish, a wide range of other species
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免疫原
Synthetic peptide corresponding to Human Sonic Hedgehog aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab21448) -
ポジティブ･コントロール
- This antibody gave a positive signal in Mouse Recombinant Sonic Hedgehog protein.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab19897の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [10H6] to Sonic Hedgehog (ab87382) as Capture.
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| WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 48 kDa).
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| 特記事項 |
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Sandwich ELISA
Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [10H6] to Sonic Hedgehog (ab87382) as Capture. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 48 kDa). |
追加情報
Is unsuitable for IHC-Fr.
ターゲット情報
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機能
Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. -
組織特異性
Expressed in fetal intestine, liver, lung, and kidney. Not expressed in adult tissues. -
関連疾患
Defects in SHH are the cause of microphthalmia isolated with coloboma type 5 (MCOPCB5) [MIM:611638]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, cataract and other abnormalities like cataract may also be present. Ocular colobomas are a set of malformations resulting from abnormal morphogenesis of the optic cup and stalk, and the fusion of the fetal fissure (optic fissure).
Defects in SHH are the cause of holoprosencephaly type 3 (HPE3) [MIM:142945]. Holoprosencephaly (HPE) [MIM:236100] is the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability. The majority of HPE3 cases are apparently sporadic, although clear examples of autosomal dominant inheritance have been described. Interestingly, up to 30% of obligate carriers of HPE3 gene in autosomal dominant pedigrees are clinically unaffected.
Defects in SHH are a cause of solitary median maxillary central incisor (SMMCI) [MIM:147250]. SMMCI is a rare dental anomaly characterized by the congenital absence of one maxillary central incisor.
Defects in SHH are the cause of triphalangeal thumb-polysyndactyly syndrome (TPTPS) [MIM:174500]. TPTPS is an autosomal dominant syndrome characterized by a wide spectrum of pre- and post-axial abnormalities due to altered SHH expression pattern during limb development. TPTPS mutations have been mapped to the 7q36 locus in the LMBR1 gene which contains in its intron 5 a long-range cis-regulatory element of SHH expression. -
配列類似性
Belongs to the hedgehog family. -
翻訳後修飾
The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity.
Cholesterylation is required for N-product targeting to lipid rafts and multimerization.
N-palmitoylation of Cys-24 by HHAT is required for N-product multimerization and full activity. -
細胞内局在
Cell membrane. The N-product either remains associated with lipid rafts at the cell surface, or forms freely diffusible active multimers with its hydrophobic lipid-modified N- and C-termini buried inside and Secreted > extracellular space. The C-terminal peptide diffuses from the cell. - Information by UniProt
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参照データベース
- Entrez Gene: 6469 Human
- Entrez Gene: 20423 Mouse
- Entrez Gene: 29499 Rat
- Entrez Gene: 398047 Xenopus laevis
- Omim: 600725 Human
- SwissProt: Q15465 Human
- SwissProt: Q62226 Mouse
- SwissProt: Q63673 Rat
see all -
別名
- HHG 1 antibody
- HHG-1 antibody
- HHG1 antibody
see all
画像
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Sandwich ELISA - Anti-Sonic Hedgehog antibody (ab19897)Standard Curve for Sonic Hedgehog (Analyte: Sonic Hedgehog protein (Amino end active) (ab63216)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [10H6] to Sonic Hedgehog (ab87382) at 1µg/ml and Detector Antibody Rabbit polyclonal to Sonic Hedgehog (ab19897) at 0.5µg/ml.
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Western blot - Anti-Sonic Hedgehog antibody (ab19897)All lanes : Anti-Sonic Hedgehog antibody (ab19897) at 1 µg/ml
Lane 1 : 22 kDa fragment of Mouse recombinant Sonic Hedgehog protein
Lane 2 : 22 kDa fragment of Mouse recombinant Sonic Hedgehog protein with Human Sonic Hedgehog peptide (ab21448) at 1 µg/ml
Lysates/proteins at 1 µg per lane.
Predicted band size: 48 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?
ab19897 detects a 180 AA (22 kDa) recombinant fragment of mouse Sonic Hedgehog (lane 1). Binding of ab19897 to Sonic Hedgehog was reduced when blocking using the immunising peptide (lane 2).
データシートおよび資料
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Datasheet download
参考文献 (20)
ab19897 は 20 報の論文で使用されています。
- Tarulli GA et al. Discrete Hedgehog Factor Expression and Action in the Developing Phallus. Int J Mol Sci 21:N/A (2020). PubMed: 32059607
- Sun Q et al. microRNA-9 and -29a regulate the progression of diabetic peripheral neuropathy via ISL1-mediated sonic hedgehog signaling pathway. Aging (Albany NY) 12:11446-11465 (2020). PubMed: 32544883
- Chen L et al. CtBP2 interacts with ZBTB18 to promote malignancy of glioblastoma. Life Sci 262:118477 (2020). PubMed: 32971103
- Sinkemani A et al. Nucleus Pulposus Cell Conditioned Medium Promotes Mesenchymal Stem Cell Differentiation into Nucleus Pulposus-Like Cells under Hypoxic Conditions. Stem Cells Int 2020:8882549 (2020). PubMed: 33424982
- Tian H & He Z Anti-hepatoma effect of taccalonolide A through suppression of sonic hedgehog pathway. Artif Cells Nanomed Biotechnol 48:939-947 (2020). PubMed: 32496832
