Anti-Somatostatin Receptor 2 抗体 [UMB1] - BSA and Azide free (ab271907)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [UMB1] to Somatostatin Receptor 2 - BSA and Azide free
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Somatostatin Receptor 2 antibody [UMB1] - BSA and Azide free
Somatostatin Receptor 2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [UMB1] to Somatostatin Receptor 2 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details
適用なし: Flow Cyt,ICC/IF or IHC-Fr -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab134152) -
ポジティブ・コントロール
- WB: Human and mouse brain tissue lysate. U937, INS-1 and Somatostatin Receptor 2 transfected HEK-293 cell lysates. IHC-P: Human pancreas, brain and breast carcinoma tissues.
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特記事項
ab271907 is the carrier-free version of ab134152.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
UMB1 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152)
- Alexa Fluor® 488 Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab311039)
- Alexa Fluor® 594 Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab311816)
- Alexa Fluor® 568 Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab313099)
- Alexa Fluor® 555 Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab313292)
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab271907の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 41 kDa.Can be blocked with Rabbit monoclonal [UMB1] to Somatostatin Receptor 2 - C-terminal (ab134152).
This antibody works better in 1%SDS Hot Lysates in WB. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).
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IP |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 41 kDa.Can be blocked with Rabbit monoclonal [UMB1] to Somatostatin Receptor 2 - C-terminal (ab134152). This antibody works better in 1%SDS Hot Lysates in WB. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).
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IP
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Receptor for somatostatins-14 and -28. This receptor is coupled via pertussis toxin sensitive G proteins to inhibition of adenylyl cyclase. In addition it stimulates phosphotyrosine phosphatase and PLC via pertussis toxin insensitive as well as sensitive G proteins. In RIN-5F cells, this receptor inhibits calcium entry by suppressing voltage dependent calcium-channels. -
組織特異性
Cerebrum and kidney. In lesser amounts in jejunum, colon and liver. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 6752 Human
- Entrez Gene: 20606 Mouse
- Entrez Gene: 54305 Rat
- Omim: 182452 Human
- SwissProt: P30874 Human
- SwissProt: P30875 Mouse
- SwissProt: P30680 Rat
- Unigene: 514451 Human
see all -
別名
- Somatostatin receptor type 2 antibody
- SRIF-1 antibody
- SRIF1 antibody
see all
画像
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Immunohistochemical analysis of formalin-fixed paraffin-embedded human pancreas labelling somatostatin receptor 2 with ab134152 at a concentration of 0.2µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32min with ULTRA cell conditioning solution (CC1 pH8.5). ab134152 anti somatostatin receptor 2 antibody was incubated at 37°C for 16min. Sections were counterstained is with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134152).
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This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134152).
Tissue Microarrays stained for Anti-Somatostatin Receptor 2 antibody [UMB1] using ab134152 in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negaive (cross mark) staining per sample type tested.The sections were incubated with ab134152 for 30 mins at room temperature used at 1:2000 dilution (0.928 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) secondary antibody. Counterstain was Hematoxylin.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labelling Somatostatin Receptor 2 with ab134152 at 1/2000 dilution (0.928 μg/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at a ready to use dilution. Positive staining on human breast carcinoma. The section was incubated with ab134152 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at a ready to use dilution.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134152).
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Immunohistochemical analysis of paraffin embedded human brain tissue labeled with ab134152 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134152). -
Immunohistochemical analysis of paraffin embedded human pancreas tissue labeled with ab134152 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134152).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab271907 は論文での使用が確認できていません。