Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
nomal goat serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
Yes - 0,3% Triton X100
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 1% DNS 0,3% Triton
Dilution
1/2000
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti Rabbit Cy3
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3
Dilution
1/1000
Additional data
Additional Notes
The test has been performed on both control mice and sst1 KO mice (Low et al, JCI, 2010) brain sections in the same experiment. Identical results have been observed (additional picture available), suggesting that the labeling observed is non specific.
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Nov 13 2013