Anti-Sodium Potassium ATPase 抗体 (ab353)
Key features and details
- Chicken polyclonal to Sodium Potassium ATPase
- Suitable for: ELISA, IHC-Fr, ICC/IF, WB
- Reacts with: Dog
- Isotype: IgY
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Sodium Potassium ATPase antibody
Sodium Potassium ATPase 一次抗体 製品一覧 -
製品の詳細
Chicken polyclonal to Sodium Potassium ATPase -
由来種
Chicken -
特異性
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum as well as purified and partially purified ATPase, Sodium, Potassium [Canine Kidney]. -
アプリケーション
適用あり: ELISA, IHC-Fr, ICC/IF, WBmore details -
種交差性
交差種: Dog -
免疫原
Full length protein corresponding to Sodium Potassium ATPase. ATPase, Sodium, Potassium [Canine Kidney]
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ポジティブ･コントロール
- ICC: MDCK cells ELISA: 1ug recombinant protein
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特記事項
Centrifuge product if not completely clear after standing at room temperature. This product is for research use only and is not intended for therapeutic or diagnostic applications. Please note that we have 2 concentrations of this antibody: 1mg/ml and 10mg/ml. Please check the vial label.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate, 0.878% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgY -
研究分野
関連製品
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab353の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
This product has been assayed against 1.0 ug of ATPase, Sodium, Potassium in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Chicken IgG [H&L] (Rabbit)and ABTS (2,2'-azino-bis-[3-ethylbenthia-zoline-6-sulfonic acid])as a substrate for 30 minutes at room temperature. |
|
| IHC-Fr |
Use at an assay dependent concentration.
(see review) |
|
| ICC/IF |
Use at an assay dependent concentration.
(see review). Use amplification. |
|
| WB |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
ELISA
Use at an assay dependent concentration. This product has been assayed against 1.0 ug of ATPase, Sodium, Potassium in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Chicken IgG [H&L] (Rabbit)and ABTS (2,2'-azino-bis-[3-ethylbenthia-zoline-6-sulfonic acid])as a substrate for 30 minutes at room temperature. |
|
IHC-Fr
Use at an assay dependent concentration. (see review) |
|
ICC/IF
Use at an assay dependent concentration. (see review). Use amplification. |
|
WB
Use at an assay dependent concentration. |
ターゲット情報
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機能
This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. -
配列類似性
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily. -
翻訳後修飾
Phosphorylation on Tyr-10 modulates pumping activity. -
細胞内局在
Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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別名
- ATPase Na+/K+ transporting alpha antibody
- adenosinetriphosphatase antibody
- AT1A1_HUMAN antibody
see all
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab353 は 10 報の論文で使用されています。
- Zámbó B et al. Clinically relevant mutations in the ABCG2 transporter uncovered by genetic analysis linked to erythrocyte membrane protein expression. Sci Rep 8:7487 (2018). ICC/IF . PubMed: 29749379
- Martín-Gago P et al. Covalent Protein Labeling at Glutamic Acids. Cell Chem Biol 24:589-597.e5 (2017). PubMed: 28434875
- Varga K et al. Histone deacetylase inhibitor- and PMA-induced upregulation of PMCA4b enhances Ca2+ clearance from MCF-7 breast cancer cells. Cell Calcium 55:78-92 (2014). PubMed: 24439526
- Braune EB et al. S/T phosphorylation of DLL1 is required for full ligand activity in vitro but dispensable for DLL1 function in vivo during embryonic patterning and marginal zone B cell development. Mol Cell Biol 34:1221-33 (2014). PubMed: 24449764
- Liu R et al. Itraconazole suppresses the growth of glioblastoma through induction of autophagy: involvement of abnormal cholesterol trafficking. Autophagy 10:1241-55 (2014). PubMed: 24905460
- Antalffy G et al. A C-terminal di-leucine motif controls plasma membrane expression of PMCA4b. Biochim Biophys Acta 1833:2561-72 (2013). ICC/IF . PubMed: 23830917
- Czepiel M et al. Differentiation of induced pluripotent stem cells into functional oligodendrocytes. Glia 59:882-92 (2011). PubMed: 21438010
- Qin Y et al. The mammalian Scribble polarity protein regulates epithelial cell adhesion and migration through E-cadherin. J Cell Biol 171:1061-71 (2005). ICC/IF ; Dog . PubMed: 16344308
- Tauscher A & Kuver R ABCG5 and ABCG8 are expressed in gallbladder epithelial cells. Biochem Biophys Res Commun 307:1021-8 (2003). IF, IHC ; Dog . PubMed: 12878214
- Lee J et al. Polarized cholesterol and phospholipid efflux in cultured gall-bladder epithelial cells: evidence for an ABCA1-mediated pathway. Biochem J 364:475-84 (2002). PubMed: 12023891