Anti-SODD 抗体 (ab2048)

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Western blot - Anti-SODD antibody (ab2048)
  • Immunocytochemistry/ Immunofluorescence - Anti-SODD antibody (ab2048)
  • Immunocytochemistry/ Immunofluorescence - Anti-SODD antibody (ab2048)

Key features and details

  • Rabbit polyclonal to SODD
  • Suitable for: ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-SODD antibody
    SODD 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to SODD

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, WBmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide corresponding to Human SODD aa 2-16.
    Sequence:

    SALRRSGYGPSDGPSC


    (Peptide available as ab8382)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • HeLa whole cell lysate or THP-1 whole cell lysate.

  • 特記事項

    SODDs.

     

    Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNF-R1 and Fas. Several novel death receptors including DR3, DR4, DR5, and DR6 were recently identified. Cell death signal is transduced by death domain containing adapter molecules through the interaction with death domain of these death receptors. A novel TNF-R1 interacting protein was recently identified and designated SODD for SODDs. SODD associates with the death domain of TNF-R1 and prevents constitutive activation of TNF-R1 signaling. TNF treatment releases SODD and permits adapter molecules such as TRADD recruiting to the active TNF-R1 complex, which activates TNF signaling pathways. SODD also interacts with DR3. SODD is ubiquitously expressed in human tissues and cell lines.

     This product was previously labelled as Silencer of Death Domain

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • バッファー

    pH: 7.2
    Preservative: 0.02% Sodium azide
  • Concentration information loading...

  • 精製度

    DEAE-Chromatography

  • 特記事項（精製）

    SODD Antibody is Antibody is DEAE purified.

  • 一次抗体 備考

    Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNF-R1 and Fas. Several novel death receptors including DR3, DR4, DR5, and DR6 were recently identified. Cell death signal is transduced by death domain containing adapter molecules through the interaction with death domain of these death receptors. A novel TNF-R1 interacting protein was recently identifiedand designated SODD for silencer of death domains. SODD associates with the death domain of TNF-R1 and prevents constitutive activation of TNF-R1 signaling. TNF treatment releases SODD and permits adapter molecules such as TRADD recruiting to the active TNF-R1 complex, which activates TNF signaling pathways. SODD also interacts with DR3. SODD is ubiquitously expressed in human tissues and cell lines.

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab2048の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
Use a concentration of 5 µg/ml.
WB
1/500 - 1/2000. Detects a band of approximately 60 kDa.
特記事項
ICC/IF
Use a concentration of 5 µg/ml.
WB
1/500 - 1/2000. Detects a band of approximately 60 kDa.

ターゲット情報

  • 機能

    Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release (By similarity). Prevents constitutive TNFRSF1A signaling.

  • 組織特異性

    Ubiquitous.

  • 配列類似性

    Contains 1 BAG domain.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    • 別名

      • BAG 4 antibody
      • BAG family molecular chaperone regulator 4 antibody
      • BAG-4 antibody
      • BAG4 antibody
      • BAG4_HUMAN antibody
      • Bcl 2 associated athanogene 4 antibody
      • Bcl-2-associated athanogene 4 antibody
      • BCL2 associated athanogene 4 antibody
      • Silencer of death domains antibody
      • SODD antibody
      see all

    画像

    • Western blot - Anti-SODD antibody (ab2048)
      Western blot - Anti-SODD antibody (ab2048)
      All lanes : Anti-SODD antibody (ab2048) at 1/500 dilution

      Lane 1 : HeLa whole cell lysate with absence of blocking peptide
      Lane 2 : THP-1 whole cell lysate with absence of blocking peptide
      Lane 3 : HeLa whole cell lysate with SODD peptide (ab8382)
      Lane 4 : THP-1 whole cell lysate with SODD peptide (ab8382)

      Observed band size: 60 kDa why is the actual band size different from the predicted?

    • Immunocytochemistry/ Immunofluorescence - Anti-SODD antibody (ab2048)
      Immunocytochemistry/ Immunofluorescence - Anti-SODD antibody (ab2048)

      ab2048 at 5µg/ml staining SODD in Hela cells by ICC/IF 

    • Immunocytochemistry/ Immunofluorescence - Anti-SODD antibody (ab2048)
      Immunocytochemistry/ Immunofluorescence - Anti-SODD antibody (ab2048)
      Immunofluorescence of SODD in Hela cells using ab2048 at 20 ug/ml.

    参考文献 (2)

    ab2048 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab2048 は 2 報の論文で使用されています。

    • Yi L  et al. Bcl-2 associated athanogene 4 promotes proliferation, migration and invasion of gastric cancer cells. Mol Med Rep 16:3753-3760 (2017). PubMed: 29067445
    • Boiani M  et al. The stress protein BAG3 stabilizes Mcl-1 protein and promotes survival of cancer cells and resistance to antagonist ABT-737. J Biol Chem 288:6980-90 (2013). WB ; Human . PubMed: 23341456

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