Anti-SODD 抗体 (ab2048)
Key features and details
- Rabbit polyclonal to SODD
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-SODD antibody
SODD 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SODD -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human SODD aa 2-16.
Sequence:SALRRSGYGPSDGPSC
(Peptide available asab8382) -
ポジティブ・コントロール
- HeLa whole cell lysate or THP-1 whole cell lysate.
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特記事項
SODDs.
Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNF-R1 and Fas. Several novel death receptors including DR3, DR4, DR5, and DR6 were recently identified. Cell death signal is transduced by death domain containing adapter molecules through the interaction with death domain of these death receptors. A novel TNF-R1 interacting protein was recently identified and designated SODD for SODDs. SODD associates with the death domain of TNF-R1 and prevents constitutive activation of TNF-R1 signaling. TNF treatment releases SODD and permits adapter molecules such as TRADD recruiting to the active TNF-R1 complex, which activates TNF signaling pathways. SODD also interacts with DR3. SODD is ubiquitously expressed in human tissues and cell lines.
This product was previously labelled as Silencer of Death Domain
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading...
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精製度
DEAE-Chromatography -
特記事項(精製)
SODD Antibody is Antibody is DEAE purified. -
一次抗体 備考
Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNF-R1 and Fas. Several novel death receptors including DR3, DR4, DR5, and DR6 were recently identified. Cell death signal is transduced by death domain containing adapter molecules through the interaction with death domain of these death receptors. A novel TNF-R1 interacting protein was recently identifiedand designated SODD for silencer of death domains. SODD associates with the death domain of TNF-R1 and prevents constitutive activation of TNF-R1 signaling. TNF treatment releases SODD and permits adapter molecules such as TRADD recruiting to the active TNF-R1 complex, which activates TNF signaling pathways. SODD also interacts with DR3. SODD is ubiquitously expressed in human tissues and cell lines. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab2048 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF | Use a concentration of 5 µg/ml. | |
WB | 1/500 - 1/2000. Detects a band of approximately 60 kDa. |
ターゲット情報
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機能
Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release (By similarity). Prevents constitutive TNFRSF1A signaling. -
組織特異性
Ubiquitous. -
配列類似性
Contains 1 BAG domain. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 9530 Human
- Entrez Gene: 67384 Mouse
- Entrez Gene: 361167 Rat
- Omim: 603884 Human
- SwissProt: O95429 Human
- SwissProt: Q8CI61 Mouse
- Unigene: 194726 Human
- Unigene: 118400 Mouse
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別名
- BAG 4 antibody
- BAG family molecular chaperone regulator 4 antibody
- BAG-4 antibody
see all
画像
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All lanes : Anti-SODD antibody (ab2048) at 1/500 dilution
Lane 1 : HeLa whole cell lysate with absence of blocking peptide
Lane 2 : THP-1 whole cell lysate with absence of blocking peptide
Lane 3 : HeLa whole cell lysate with SODD peptide (ab8382)
Lane 4 : THP-1 whole cell lysate with SODD peptide (ab8382)
Observed band size: 60 kDa why is the actual band size different from the predicted? -
ab2048 at 5µg/ml staining SODD in Hela cells by ICC/IF
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Immunofluorescence of SODD in Hela cells using ab2048 at 20 ug/ml.
プロトコール
参考文献 (2)
ab2048 は 2 報の論文で使用されています。
- Yi L et al. Bcl-2 associated athanogene 4 promotes proliferation, migration and invasion of gastric cancer cells. Mol Med Rep 16:3753-3760 (2017). PubMed: 29067445
- Boiani M et al. The stress protein BAG3 stabilizes Mcl-1 protein and promotes survival of cancer cells and resistance to antagonist ABT-737. J Biol Chem 288:6980-90 (2013). WB ; Human . PubMed: 23341456