Anti-SODD 抗体 (ab2048)
Key features and details
- Rabbit polyclonal to SODD
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-SODD antibody
SODD 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SODD -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human SODD aa 2-16.
Sequence:SALRRSGYGPSDGPSC
(Peptide available asab8382) -
ポジティブ･コントロール
- HeLa whole cell lysate or THP-1 whole cell lysate.
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特記事項
Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNF-R1 and Fas. Several novel death receptors including DR3, DR4, DR5, and DR6 were recently identified. Cell death signal is transduced by death domain containing adapter molecules through the interaction with death domain of these death receptors. A novel TNF-R1 interacting protein was recently identified and designated SODD for SODDs. SODD associates with the death domain of TNF-R1 and prevents constitutive activation of TNF-R1 signaling. TNF treatment releases SODD and permits adapter molecules such as TRADD recruiting to the active TNF-R1 complex, which activates TNF signaling pathways. SODD also interacts with DR3. SODD is ubiquitously expressed in human tissues and cell lines.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
精製度
DEAE-Chromatography -
特記事項（精製）
SODD Antibody is Antibody is DEAE purified. -
一次抗体 備考
Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNF-R1 and Fas. Several novel death receptors including DR3, DR4, DR5, and DR6 were recently identified. Cell death signal is transduced by death domain containing adapter molecules through the interaction with death domain of these death receptors. A novel TNF-R1 interacting protein was recently identifiedand designated SODD for silencer of death domains. SODD associates with the death domain of TNF-R1 and prevents constitutive activation of TNF-R1 signaling. TNF treatment releases SODD and permits adapter molecules such as TRADD recruiting to the active TNF-R1 complex, which activates TNF signaling pathways. SODD also interacts with DR3. SODD is ubiquitously expressed in human tissues and cell lines. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab2048の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 5 µg/ml.
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| WB |
1/500 - 1/2000. Detects a band of approximately 60 kDa.
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| 特記事項 |
|---|
|
ICC/IF
Use a concentration of 5 µg/ml. |
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WB
1/500 - 1/2000. Detects a band of approximately 60 kDa. |
ターゲット情報
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機能
Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release (By similarity). Prevents constitutive TNFRSF1A signaling. -
組織特異性
Ubiquitous. -
配列類似性
Contains 1 BAG domain. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 9530 Human
- Omim: 603884 Human
- SwissProt: O95429 Human
- Unigene: 194726 Human
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別名
- BAG 4 antibody
- BAG family molecular chaperone regulator 4 antibody
- BAG-4 antibody
see all
画像
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Western blot - Anti-SODD antibody (ab2048)All lanes : Anti-SODD antibody (ab2048) at 1/500 dilution
Lane 1 : HeLa whole cell lysate with absence of blocking peptide
Lane 2 : THP-1 whole cell lysate with absence of blocking peptide
Lane 3 : HeLa whole cell lysate with SODD peptide (ab8382)
Lane 4 : THP-1 whole cell lysate with SODD peptide (ab8382)
Observed band size: 60 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-SODD antibody (ab2048)ab2048 at 5µg/ml staining SODD in Hela cells by ICC/IF
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Immunocytochemistry/ Immunofluorescence - Anti-SODD antibody (ab2048)Immunofluorescence of SODD in Hela cells using ab2048 at 20 ug/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab2048 は 2 報の論文で使用されています。
- Yi L et al. Bcl-2 associated athanogene 4 promotes proliferation, migration and invasion of gastric cancer cells. Mol Med Rep 16:3753-3760 (2017). PubMed: 29067445
- Boiani M et al. The stress protein BAG3 stabilizes Mcl-1 protein and promotes survival of cancer cells and resistance to antagonist ABT-737. J Biol Chem 288:6980-90 (2013). WB ; Human . PubMed: 23341456