Anti-SODD 抗体 (ab2048)

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製品の概要

  • 製品名

    Anti-SODD antibody
    SODD 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to SODD

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, WBmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide corresponding to Human SODD aa 2-16.
    Sequence:

    SALRRSGYGPSDGPSC


    (Peptide available as ab8382)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • HeLa whole cell lysate or THP-1 whole cell lysate.

  • 特記事項

    SODDs.

     

    Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNF-R1 and Fas. Several novel death receptors including DR3, DR4, DR5, and DR6 were recently identified. Cell death signal is transduced by death domain containing adapter molecules through the interaction with death domain of these death receptors. A novel TNF-R1 interacting protein was recently identified and designated SODD for SODDs. SODD associates with the death domain of TNF-R1 and prevents constitutive activation of TNF-R1 signaling. TNF treatment releases SODD and permits adapter molecules such as TRADD recruiting to the active TNF-R1 complex, which activates TNF signaling pathways. SODD also interacts with DR3. SODD is ubiquitously expressed in human tissues and cell lines.

     This product was previously labelled as Silencer of Death Domain

     

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • バッファー

    Preservative: 0.02% Sodium azide
  • Concentration information loading...

  • 精製度

    DEAE-Chromatography

  • 特記事項（精製）

    SODD Antibody is Antibody is DEAE purified.

  • 一次抗体 備考

    Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNF-R1 and Fas. Several novel death receptors including DR3, DR4, DR5, and DR6 were recently identified. Cell death signal is transduced by death domain containing adapter molecules through the interaction with death domain of these death receptors. A novel TNF-R1 interacting protein was recently identifiedand designated SODD for silencer of death domains. SODD associates with the death domain of TNF-R1 and prevents constitutive activation of TNF-R1 signaling. TNF treatment releases SODD and permits adapter molecules such as TRADD recruiting to the active TNF-R1 complex, which activates TNF signaling pathways. SODD also interacts with DR3. SODD is ubiquitously expressed in human tissues and cell lines.

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab2048 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/2000. Detects a band of approximately 60 kDa.

ターゲット情報

画像

  • Western blot - Anti-SODD antibody (ab2048)
    Western blot - Anti-SODD antibody (ab2048)
    All lanes : Anti-SODD antibody (ab2048) at 1/500 dilution

    Lane 1 : HeLa whole cell lysate with absence of blocking peptide
    Lane 2 : THP-1 whole cell lysate with absence of blocking peptide
    Lane 3 : HeLa whole cell lysate with SODD peptide (ab8382)
    Lane 4 : THP-1 whole cell lysate with SODD peptide (ab8382)

    Observed band size: 60 kDa
    why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-SODD antibody (ab2048)
    Immunocytochemistry/ Immunofluorescence - Anti-SODD antibody (ab2048)

    ab2048 at 5µg/ml staining SODD in Hela cells by ICC/IF 

  • Immunocytochemistry/ Immunofluorescence - Anti-SODD antibody (ab2048)
    Immunocytochemistry/ Immunofluorescence - Anti-SODD antibody (ab2048)
    Immunofluorescence of SODD in Hela cells using ab2048 at 20 ug/ml.

参考文献

ab2048 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab2048 は 2 報の論文で使用されています。

  • Yi L  et al. Bcl-2 associated athanogene 4 promotes proliferation, migration and invasion of gastric cancer cells. Mol Med Rep 16:3753-3760 (2017). PubMed: 29067445
  • Boiani M  et al. The stress protein BAG3 stabilizes Mcl-1 protein and promotes survival of cancer cells and resistance to antagonist ABT-737. J Biol Chem 288:6980-90 (2013). WB ; Human . PubMed: 23341456

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