Anti-SOD2/MnSOD 抗体 (ab13533)
Key features and details
- Rabbit polyclonal to SOD2/MnSOD
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-SOD2/MnSOD antibody
SOD2/MnSOD 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SOD2/MnSOD -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat
交差が予測される動物種: Human
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免疫原
Recombinant fragment corresponding to Human SOD2/MnSOD.
Database link: P04179 -
ポジティブ･コントロール
- WB: Mouse Brain ; Rat Brain ; Rat Brain Membrane; Mouse Liver ; Mouse Hepatocytes
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.1% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This antibody was immunoaffinity purified. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab13533の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (22) |
1/5000. Detects a band of approximately 25 kDa (predicted molecular weight: 26.6 kDa).
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| 特記事項 |
|---|
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WB
1/5000. Detects a band of approximately 25 kDa (predicted molecular weight: 26.6 kDa). |
ターゲット情報
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機能
Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems. -
関連疾患
Genetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6) [MIM:612634]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. -
配列類似性
Belongs to the iron/manganese superoxide dismutase family. -
翻訳後修飾
Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity. -
細胞内局在
Mitochondrion matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 6648 Human
- Entrez Gene: 20656 Mouse
- Entrez Gene: 24787 Rat
- Omim: 147460 Human
- SwissProt: P04179 Human
- SwissProt: P09671 Mouse
- SwissProt: P07895 Rat
- Unigene: 487046 Human
see all -
別名
- Indophenoloxidase B antibody
- IPO B antibody
- IPOB antibody
see all
画像
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Western blot - Anti-SOD2/MnSOD antibody (ab13533)All lanes : Anti-SOD2/MnSOD antibody (ab13533) at 1/1000 dilution (o/n at 4degC)
Lane 1 : Mouse brain
Lane 2 : Rat brain
Lane 3 : Rat brain membrane
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP for 1 hr at RT with shaking at 1/5000 dilution
Predicted band size: 26.6 kDaBlock: 5% Skim Milk powder in TBST.
Color Development: Chemiluminescent for HRP for 5 min at RT.
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Western blot - Anti-SOD2/MnSOD antibody (ab13533)Image courtesy of anonymous abreviewerAll lanes : Anti-SOD2/MnSOD antibody (ab13533) at 1/5000 dilution
Lane 1 : mouse liver whole cell lysate
Lane 2 : mouse hepatocytes whole cell lysate
Secondary
All lanes : Goat anti-rabbit HRP conjugate at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 26.6 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
Exposure time: 15 seconds
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Western blot - Anti-SOD2/MnSOD antibody (ab13533)This image is courtesy of an Abreview submitted by Dr sandra sobocanecAll lanes : Anti-SOD2/MnSOD antibody (ab13533) at 1/5000 dilution
Lane 1 : 2ug mouse liver homogenate supernatant
Lane 2 : 1ug mouse liver homogenate supernatant
Lane 3 : 0.5ug mouse liver homogenate supernatant
Secondary
All lanes : HRP conjugated donkey anti-rabbit antibody
Performed under reducing conditions.
Predicted band size: 26.6 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (260)
ab13533 は 260 報の論文で使用されています。
- DeJulius CR et al. Microsphere antioxidant and sustained erythropoietin-R76E release functions cooperate to reduce traumatic optic neuropathy. J Control Release 329:762-773 (2021). PubMed: 33049330
- Wanschel ACBA et al. The Presence of Cholesteryl Ester Transfer Protein (CETP) in Endothelial Cells Generates Vascular Oxidative Stress and Endothelial Dysfunction. Biomolecules 11:N/A (2021). PubMed: 33430172
- Naguib S et al. Retinal oxidative stress activates the NRF2/ARE pathway: An early endogenous protective response to ocular hypertension. Redox Biol 42:101883 (2021). PubMed: 33579667
- Su H et al. Identification of hub genes associated with neutrophils infiltration in colorectal cancer. J Cell Mol Med 25:3371-3380 (2021). PubMed: 33666342
- Satriyo PB et al. 4-Acetylantroquinonol B induced DNA damage response signaling and apoptosis via suppressing CDK2/CDK4 expression in triple negative breast cancer cells. Toxicol Appl Pharmacol 422:115493 (2021). PubMed: 33727089