アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
お問い合わせは電話 +81-(0)3-6231-0940 または メールでどうぞ

  • アカウント
  • ログアウト
ログイン または 新規登録

Welcome

ログイン または

すでにアカウントをお持ちですか?

新規登録
カート
クイック・オーダー
Abcam homepage

  • 研究用製品
    製品タイプ別
    一次抗体
    二次抗体
    イムノアッセイキット・試薬
    細胞・組織イメージング
    細胞・バイオケミカルアッセイ
    タンパク質・ペプチド
    製品タイプ別
    プロテオミクス
    アゴニスト・アクチベータ-・アンタゴニスト・インヒビター
    細胞株・ライゼート
    miRNA マルチプレックス・アッセイキット
    マルチプレックス・アッセイ
    研究分野別
    癌
    循環器系
    細胞生物学
    エピジェネティクス
    メタボリズム
    発生生物学
    研究分野別
    免疫学
    微生物学
    脳神経科学
    シグナル伝達
    ステムセル
  • カスタム製品 & パートナーシップ
    カスタム製品 & パートナーシップ

    診断や治療プログラムを加速するための、カスタム製品および商業化に向けたパートナーシップ

    カスタム製品

    私たちとパートナーになる

  • サポート
    サポートハブ

    研究のためのサポートとアドバイス

    サポートハブを表示

    プロトコール

    実験のステップごとの詳細

    プロトコールを表示

  • イベント
    • イベント・カレンダー
    • 癌
    • 循環器
    • エピジェネティクス&核内情報
    • 免疫学
    • ニューロサイエンス
    • ステムセル
    • 展示会
    • ウェビナー
    最新のイベント情報

    世界中でアブカムが主催する研究会やセミナーの日程、内容、演者など

    イベント・カレンダー

  • パスウェイ
    細胞シグナル伝達パスウェイ

    すべてのパスウェイを見る

    インタラクティブ・パスウェイを見る

  1. Link

    sod2mnsod-antibody-ab13533.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cell Biology Apoptosis Mitochondrial
Share by email

Anti-SOD2/MnSOD 抗体 (ab13533)

  • Datasheet
  • SDS
Reviews (30)Q&A (10)References (260)

Product price, shipping and contact information

Currently unavailable

申し訳ございませんが、現在表示できません。

こちらにお問い合わせいただくか、またはしばらく経ってから改めてお試しください

 

Loading size & price…

 

出荷および受注について

Shipping info

Promotion Information

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-SOD2/MnSOD antibody (ab13533)
  • Western blot - Anti-SOD2/MnSOD antibody (ab13533)
  • Western blot - Anti-SOD2/MnSOD antibody (ab13533)

Key features and details

  • Rabbit polyclonal to SOD2/MnSOD
  • Suitable for: WB
  • Reacts with: Mouse, Rat
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-SOD2/MnSOD antibody
    SOD2/MnSOD 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to SOD2/MnSOD
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: WBmore details
  • 種交差性

    交差種: Mouse, Rat
    交差が予測される動物種: Human
  • 免疫原

    Recombinant fragment corresponding to Human SOD2/MnSOD.
    Database link: P04179

  • ポジティブ・コントロール

    • WB: Mouse Brain ; Rat Brain ; Rat Brain Membrane; Mouse Liver ; Mouse Hepatocytes
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー

    Preservative: 0.1% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • 特記事項(精製)

    This antibody was immunoaffinity purified.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Cell Biology
    • Apoptosis
    • Mitochondrial
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Cardiovascular
    • Vasculature
    • Endothelium
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Cancer
    • Cancer Metabolism
    • Cellular metabolic process
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Antioxidants
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Metabolism
    • Types of disease
    • Cancer
    • Cancer
    • Cell Death
    • Apoptosis
    • Mitochondrial
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab13533の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (22)
1/5000. Detects a band of approximately 25 kDa (predicted molecular weight: 26.6 kDa).
特記事項
WB
1/5000. Detects a band of approximately 25 kDa (predicted molecular weight: 26.6 kDa).

ターゲット情報

  • 機能

    Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
  • 関連疾患

    Genetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6) [MIM:612634]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • 配列類似性

    Belongs to the iron/manganese superoxide dismutase family.
  • 翻訳後修飾

    Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity.
  • 細胞内局在

    Mitochondrion matrix.
  • Target information above from: UniProt accession P04179 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 6648 Human
    • Entrez Gene: 20656 Mouse
    • Entrez Gene: 24787 Rat
    • Omim: 147460 Human
    • SwissProt: P04179 Human
    • SwissProt: P09671 Mouse
    • SwissProt: P07895 Rat
    • Unigene: 487046 Human
    • Unigene: 290876 Mouse
    • Unigene: 10488 Rat
    see all
  • 別名

    • Indophenoloxidase B antibody
    • IPO B antibody
    • IPOB antibody
    • Manganese containing superoxide dismutase antibody
    • Manganese SOD antibody
    • Manganese superoxide dismutase antibody
    • Mangano superoxide dismutase antibody
    • Mn SOD antibody
    • Mn superoxide dismutase antibody
    • MNSOD antibody
    • MVCD6 antibody
    • SOD 2 antibody
    • SOD2 antibody
    • SODM_HUMAN antibody
    • Superoxide dismutase [Mn] mitochondrial antibody
    • Superoxide dismutase [Mn], mitochondrial antibody
    • Superoxide dismutase 2 mitochondrial antibody
    see all

画像

  • Western blot - Anti-SOD2/MnSOD antibody (ab13533)
    Western blot - Anti-SOD2/MnSOD antibody (ab13533)
    All lanes : Anti-SOD2/MnSOD antibody (ab13533) at 1/1000 dilution (o/n at 4degC)

    Lane 1 : Mouse brain
    Lane 2 : Rat brain
    Lane 3 : Rat brain membrane

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG HRP for 1 hr at RT with shaking at 1/5000 dilution

    Predicted band size: 26.6 kDa



    Block: 5% Skim Milk powder in TBST.

    Color Development: Chemiluminescent for HRP for 5 min at RT.

  • Western blot - Anti-SOD2/MnSOD antibody (ab13533)
    Western blot - Anti-SOD2/MnSOD antibody (ab13533)Image courtesy of anonymous abreviewer
    All lanes : Anti-SOD2/MnSOD antibody (ab13533) at 1/5000 dilution

    Lane 1 : mouse liver whole cell lysate
    Lane 2 : mouse hepatocytes whole cell lysate

    Secondary
    All lanes : Goat anti-rabbit HRP conjugate at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 26.6 kDa
    Observed band size: 24 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds

    See Abreview

  • Western blot - Anti-SOD2/MnSOD antibody (ab13533)
    Western blot - Anti-SOD2/MnSOD antibody (ab13533)This image is courtesy of an Abreview submitted by Dr sandra sobocanec
    All lanes : Anti-SOD2/MnSOD antibody (ab13533) at 1/5000 dilution

    Lane 1 : 2ug mouse liver homogenate supernatant
    Lane 2 : 1ug mouse liver homogenate supernatant
    Lane 3 : 0.5ug mouse liver homogenate supernatant

    Secondary
    All lanes : HRP conjugated donkey anti-rabbit antibody

    Performed under reducing conditions.

    Predicted band size: 26.6 kDa
    Observed band size: 25 kDa why is the actual band size different from the predicted?


    Exposure time: 10 seconds

    See Abreview

プロトコール

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (260)

ab13533 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab13533 は 260 報の論文で使用されています。

  • DeJulius CR  et al. Microsphere antioxidant and sustained erythropoietin-R76E release functions cooperate to reduce traumatic optic neuropathy. J Control Release 329:762-773 (2021). PubMed: 33049330
  • Wanschel ACBA  et al. The Presence of Cholesteryl Ester Transfer Protein (CETP) in Endothelial Cells Generates Vascular Oxidative Stress and Endothelial Dysfunction. Biomolecules 11:N/A (2021). PubMed: 33430172
  • Naguib S  et al. Retinal oxidative stress activates the NRF2/ARE pathway: An early endogenous protective response to ocular hypertension. Redox Biol 42:101883 (2021). PubMed: 33579667
  • Su H  et al. Identification of hub genes associated with neutrophils infiltration in colorectal cancer. J Cell Mol Med 25:3371-3380 (2021). PubMed: 33666342
  • Satriyo PB  et al. 4-Acetylantroquinonol B induced DNA damage response signaling and apoptosis via suppressing CDK2/CDK4 expression in triple negative breast cancer cells. Toxicol Appl Pharmacol 422:115493 (2021). PubMed: 33727089
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-10 of 40 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SOD2/MnSOD antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Specification
skin
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2%
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 Apr 28 2021

Western blot abreview for Anti-SOD2/MnSOD antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (brain)
Gel Running Conditions
Reduced Denaturing (TEO TRICINE 4-20% gel (ab119209))
Loading amount
20 µg
Specification
brain
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Dr. Yonatan Feuermann

Verified customer

投稿 Apr 12 2021

Western blot abreview for Anti-SOD2/MnSOD antibody

Excellent
Abreviews
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
25 µg
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Jan 03 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-SOD2/MnSOD antibody

Good
Abreviews
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (cerebellum)
Permeabilization
Yes - Triton
Specification
cerebellum
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 Oct 25 2019

Immunocytochemistry/ Immunofluorescence abreview for Anti-SOD2/MnSOD antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293T)
Permeabilization
Yes - 0.1% Triton X-100
Specification
HEK293T
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 Jul 17 2017

Western blot abreview for Anti-SOD2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Epithelial OVCA cells)
Gel Running Conditions
Reduced Denaturing (12.5%)
Loading amount
75 µg
Treatment
0- 100µM Perifosine 24 hr
Specification
Epithelial OVCA cells
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More

Abcam user community

Verified customer

投稿 Aug 09 2016

Western blot abreview for Anti-SOD2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Bone tissue (Tibia))
Gel Running Conditions
Reduced Denaturing (12,5%)
Loading amount
50 µg
Specification
Bone tissue (Tibia)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

Abcam user community

Verified customer

投稿 Aug 25 2015

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SOD2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Rat gonad)
Antigen retrieval step
Enzymatic
Permeabilization
No
Specification
Rat gonad
Blocking step
Serum as blocking agent for 12 hour(s) and 45 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Apr 14 2015

Western blot abreview for Anti-SOD2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (liver, hepatocytes)
Specification
liver, hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More

Abcam user community

Verified customer

投稿 Jan 05 2015

Western blot abreview for Anti-SOD2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
80 µg
Gel Running Conditions
Reduced Denaturing (12.5% Acrylamide, 2D gel ph 3-10)
Sample
Pig Tissue lysate - other (Muscle Sarcoplasm)
Specification
Muscle Sarcoplasm
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Dr. Shannon Cruzen

Verified customer

投稿 Sep 29 2014

1-10 of 40 Abreviews or Q&A

  •  戻る
  • 1
  • 2
  • 3
  • 4
  • 次へ 

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

技術情報や製品情報をメールで受け取る 登録
製品・研究分野別
  • 癌
  • 循環器
  • 細胞生物学
  • 発生
  • エピジェネティクス&核内情報
  • 免疫学
  • メタボリズム
  • 感染症
  • ニューロサイエンス
  • シグナル伝達
  • ステムセル
製品・タイプ別
  • 一次抗体
  • 二次抗体
  • バイオケミカルズ
  • アイソタイプ・コントロール
  • マルチカラー・セレクター
  • キット
  • ローディング・コントロール
  • ライゼート
  • ペプチド
  • タンパク質
  • スライド
  • タグ抗体・細胞マーカー
  • その他試薬
サービス&サポート
  • サポート
  • よくあるお問い合わせ
  • プロトコール&トラブルシュート
  • ご購入に関して
  • お問い合わせフォーム
  • トレーニング
  • ターゲット別に見る
アブカムについて
  • 企業ウェブサイト
  • 投資家の皆様へ
  • 企業情報
  • 採用情報
  • アブカムについて
イベント
  • 展示会
  • カンファレンス
International websites
  • abcam.com
  • abcam.cn

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
© 1998-2022 Abcam plc. All rights reserved.