製品の概要

  • 製品名
    Anti-SOD2/MnSOD antibody
    SOD2/MnSOD 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SOD2/MnSOD
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: ELISA, IHC-Fr, ICC/IF, IHC-P, WB, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Monkey, Gerbil
  • 免疫原

    Full length protein corresponding to Human SOD2/MnSOD.

  • ポジティブ･コントロール
    • Rat brain tissue extract.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab13533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/150.
IHC-P Use at an assay dependent concentration.
WB 1/5000. Detects a band of approximately 25 kDa (predicted molecular weight: 26.6 kDa).
IP Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能
    Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
  • 関連疾患
    Genetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6) [MIM:612634]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • 配列類似性
    Belongs to the iron/manganese superoxide dismutase family.
  • 翻訳後修飾
    Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity.
  • 細胞内局在
    Mitochondrion matrix.
  • Information by UniProt
  • 参照データベース
    see all
  • 別名
    • Indophenoloxidase B antibody
    • IPO B antibody
    • IPOB antibody
    • Manganese containing superoxide dismutase antibody
    • Manganese SOD antibody
    • Manganese superoxide dismutase antibody
    • Mangano superoxide dismutase antibody
    • Mn SOD antibody
    • Mn superoxide dismutase antibody
    • MNSOD antibody
    • MVCD6 antibody
    • SOD 2 antibody
    • SOD2 antibody
    • SODM_HUMAN antibody
    • Superoxide dismutase [Mn] mitochondrial antibody
    • Superoxide dismutase [Mn], mitochondrial antibody
    • Superoxide dismutase 2 mitochondrial antibody
    see all

画像

  • Western blot - Anti-SOD2/MnSOD antibody (ab13533)
    Western blot - Anti-SOD2/MnSOD antibody (ab13533)Image courtesy of anonymous abreviewer
    All lanes : Anti-SOD2/MnSOD antibody (ab13533) at 1/5000 dilution

    Lane 1 : mouse liver whole cell lysate
    Lane 2 : mouse hepatocytes whole cell lysate

    Secondary
    All lanes : Goat anti-rabbit HRP conjugate at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 26.6 kDa
    Observed band size: 24 kDa (why is the actual band size different from the predicted?)


    Exposure time: 15 seconds

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-SOD2/MnSOD antibody (ab13533)
    Immunocytochemistry/ Immunofluorescence - Anti-SOD2/MnSOD antibody (ab13533)This image is courtesy of an Abreview submitted by Aurelien Bayot
    ab13533 staining Superoxide Dismutase 2 in Human normal fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 1% BSA for 1 hour at 37°C. Samples were incubated with primary antibody (1/150 in 1% BSA) for 2 hours at 37°C. An undiluted Alexa Fluor®488-conjugated Donkey anti-rabbit IgG polyclonal was used as the secondary antibody. Nuclei were counterstained with DAPI.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD antibody (ab13533)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD antibody (ab13533)

    ab13533 at 1/100 dilution staining Superoxide Dismutase in rat muscle fibre tissue section by Immunohistochemistry (Bouin's fixative fixed paraffin-embedded tissue section). Antigen retrieval was done by microwave in citrate buffer. A goat anti-rabbit secondary was used at 1/10 dilution.

  • Western blot - Anti-SOD2/MnSOD antibody (ab13533)
    Western blot - Anti-SOD2/MnSOD antibody (ab13533)This image is courtesy of an Abreview submitted by Dr sandra sobocanec
    All lanes : Anti-SOD2/MnSOD antibody (ab13533) at 1/5000 dilution

    Lane 1 : 2ug mouse liver homogenate supernatant
    Lane 2 : 1ug mouse liver homogenate supernatant
    Lane 3 : 0.5ug mouse liver homogenate supernatant

    Secondary
    All lanes : HRP conjugated donkey anti-rabbit antibody

    Performed under reducing conditions.

    Predicted band size: 26.6 kDa
    Observed band size: 25 kDa (why is the actual band size different from the predicted?)


    Exposure time: 10 seconds

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-SOD2/MnSOD antibody (ab13533)
    Immunocytochemistry/ Immunofluorescence - Anti-SOD2/MnSOD antibody (ab13533)This image is courtesy of an Abreview submitted by Dr jp lu

    ab13533 staining Superoxide Dismutase 2 in human Hella cells by ICC/IF. Cells were acetone fixed and blocked in 5% serum for 1 hour at 24°C. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 24°C. The secondary antibody used was Alexa Fluor®594 donkey anti-rabbit diluted at 1/100.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-SOD2/MnSOD antibody (ab13533)
    Immunocytochemistry/ Immunofluorescence - Anti-SOD2/MnSOD antibody (ab13533)This image is courtesy of an Abreview submitted by Dr Anne Chiaramello

    ab13533 staining NGF-differentiated PC12 cells (rat) by ICC/IF.  Cells were PFA fixed and permeabilized in 0.2% Triton X-100 prior to blocking in 10% serum for 1 hour at 25°C.  The primary antibody was diluted to 2µg/ml in PBS and incubated with the sample for 1 hour at 25°C.  An Alexa Fluor® 488 conjugated goat anti-rabbit antibody was used as the secondary.

    See Abreview

参考文献

This product has been referenced in:
  • Jeong BY  et al. Oxidative stress caused by activation of NADPH oxidase 4 promotes contrast-induced acute kidney injury. PLoS One 13:e0191034 (2018). Read more (PubMed: 29329317) »
  • Pinteric M  et al. De novo expression of transfected sirtuin 3 enhances susceptibility of human MCF-7 breast cancer cells to hyperoxia treatment. Free Radic Res N/A:1-13 (2018). Read more (PubMed: 29683756) »

See all 88 Publications for this product

ab13533 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

レビューを送る 質問を送る

36 reviews or Q&A

10
Votes: Highest First

Immunocytochemistry/ Immunofluorescence abreview for Anti-SOD2/MnSOD antibody

 Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293T)
Permeabilization
Yes - 0.1% Triton X-100
Specification
HEK293T
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Read More
abreview image
Username

Abcam user community

Verified customer

投稿 Jul 17 2017

Western blot abreview for Anti-SOD2 antibody

 Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Epithelial OVCA cells)
Gel Running Conditions
Reduced Denaturing (12.5%)
Loading amount
75 µg
Treatment
0- 100µM Perifosine 24 hr
Specification
Epithelial OVCA cells
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More
abreview image
Username

Abcam user community

Verified customer

投稿 Aug 09 2016

Western blot abreview for Anti-SOD2 antibody

 Excellent
Abreviews
Application
Western blot
Sample
Rat Tissue lysate - whole (Bone tissue (Tibia))
Gel Running Conditions
Reduced Denaturing (12,5%)
Loading amount
50 µg
Specification
Bone tissue (Tibia)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
abreview image
Username

Abcam user community

Verified customer

投稿 Aug 25 2015

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SOD2 antibody

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Rat gonad)
Antigen retrieval step
Enzymatic
Permeabilization
No
Specification
Rat gonad
Blocking step
Serum as blocking agent for 12 hour(s) and 45 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
abreview image
Username

Abcam user community

Verified customer

投稿 Apr 14 2015

Western blot abreview for Anti-SOD2 antibody

 Good
Abreviews
Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (liver, hepatocytes)
Specification
liver, hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
abreview image
Username

Abcam user community

Verified customer

投稿 Jan 05 2015

Western blot abreview for Anti-SOD2 antibody

 Excellent
Abreviews
Application
Western blot
Loading amount
80 µg
Gel Running Conditions
Reduced Denaturing (12.5% Acrylamide, 2D gel ph 3-10)
Sample
Pig Tissue lysate - other (Muscle Sarcoplasm)
Specification
Muscle Sarcoplasm
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
abreview image
Username

Dr. Shannon Cruzen

Verified customer

投稿 Sep 29 2014

Western blot abreview for Anti-SOD2 antibody

 Excellent
Abreviews
Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (10% SDS PAGE)
Sample
Mouse Tissue lysate - whole (Liver)
Specification
Liver
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
abreview image
Username

Abcam user community

Verified customer

投稿 Jul 02 2014

Western blot abreview for Anti-SOD2 antibody

 Excellent
Abreviews
Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (4-20%)
Sample
Mouse Tissue lysate - whole (Lung)
Specification
Lung
Treatment
S. aureus 10^7 CFU
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
abreview image
Username

Mr. Alan Chang

Verified customer

投稿 Mar 21 2014

Western blot abreview for Anti-SOD2 antibody

 Excellent
Abreviews
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (10% separating gel)
Sample
Rat Tissue lysate - whole (cerebellum)
Specification
cerebellum
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
abreview image
Username

Abcam user community

Verified customer

投稿 Feb 21 2014

Western blot abreview for Anti-SOD2 antibody

 Excellent
Abreviews
Application
Western blot
Loading amount
20000 cells
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - whole cell (jurkats)
Specification
jurkats
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Read More
abreview image
Username

Dr. Tejpal Gill

Verified customer

投稿 Jan 13 2014

1-10 of 36 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録