Anti-SOD2/MnSOD 抗体 (ab13533)
製品の概要
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製品名
Anti-SOD2/MnSOD antibody
SOD2/MnSOD 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SOD2/MnSOD -
由来種
Rabbit -
アプリケーション
適用あり: ELISA, IHC-Fr, ICC/IF, IHC-P, WB, IPmore details -
種交差性
交差種: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Monkey, Gerbil -
免疫原
Full length protein corresponding to Human SOD2/MnSOD.
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ポジティブ･コントロール
- Rat brain tissue extract.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.1% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This antibody was immunoaffinity purified. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
Our Abpromise guarantee covers the use of ab13533 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| ICC/IF | 1/150. | |
| IHC-P | Use at an assay dependent concentration. | |
| WB | 1/5000. Detects a band of approximately 25 kDa (predicted molecular weight: 26.6 kDa). | |
| IP | Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems. -
関連疾患
Genetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6) [MIM:612634]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. -
配列類似性
Belongs to the iron/manganese superoxide dismutase family. -
翻訳後修飾
Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity. -
細胞内局在
Mitochondrion matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 6648 Human
- Entrez Gene: 20656 Mouse
- Entrez Gene: 24787 Rat
- Omim: 147460 Human
- SwissProt: P04179 Human
- SwissProt: P09671 Mouse
- SwissProt: P07895 Rat
- Unigene: 487046 Human
see all -
別名
- Indophenoloxidase B antibody
- IPO B antibody
- IPOB antibody
see all
画像
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Western blot - Anti-SOD2/MnSOD antibody (ab13533)Image courtesy of anonymous abreviewerAll lanes : Anti-SOD2/MnSOD antibody (ab13533) at 1/5000 dilution
Lane 1 : mouse liver whole cell lysate
Lane 2 : mouse hepatocytes whole cell lysate
Secondary
All lanes : Goat anti-rabbit HRP conjugate at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 26.6 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
Exposure time: 15 seconds
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Immunocytochemistry/ Immunofluorescence - Anti-SOD2/MnSOD antibody (ab13533)This image is courtesy of an Abreview submitted by Aurelien Bayotab13533 staining Superoxide Dismutase 2 in Human normal fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 1% BSA for 1 hour at 37°C. Samples were incubated with primary antibody (1/150 in 1% BSA) for 2 hours at 37°C. An undiluted Alexa Fluor®488-conjugated Donkey anti-rabbit IgG polyclonal was used as the secondary antibody. Nuclei were counterstained with DAPI. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD antibody (ab13533)ab13533 at 1/100 dilution staining Superoxide Dismutase in rat muscle fibre tissue section by Immunohistochemistry (Bouin's fixative fixed paraffin-embedded tissue section). Antigen retrieval was done by microwave in citrate buffer. A goat anti-rabbit secondary was used at 1/10 dilution.
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Western blot - Anti-SOD2/MnSOD antibody (ab13533)This image is courtesy of an Abreview submitted by Dr sandra sobocanecAll lanes : Anti-SOD2/MnSOD antibody (ab13533) at 1/5000 dilution
Lane 1 : 2ug mouse liver homogenate supernatant
Lane 2 : 1ug mouse liver homogenate supernatant
Lane 3 : 0.5ug mouse liver homogenate supernatant
Secondary
All lanes : HRP conjugated donkey anti-rabbit antibody
Performed under reducing conditions.
Predicted band size: 26.6 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds
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Immunocytochemistry/ Immunofluorescence - Anti-SOD2/MnSOD antibody (ab13533)This image is courtesy of an Abreview submitted by Dr jp luab13533 staining Superoxide Dismutase 2 in human Hella cells by ICC/IF. Cells were acetone fixed and blocked in 5% serum for 1 hour at 24°C. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 24°C. The secondary antibody used was Alexa Fluor®594 donkey anti-rabbit diluted at 1/100.
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Immunocytochemistry/ Immunofluorescence - Anti-SOD2/MnSOD antibody (ab13533)This image is courtesy of an Abreview submitted by Dr Anne Chiaramelloab13533 staining NGF-differentiated PC12 cells (rat) by ICC/IF. Cells were PFA fixed and permeabilized in 0.2% Triton X-100 prior to blocking in 10% serum for 1 hour at 25°C. The primary antibody was diluted to 2µg/ml in PBS and incubated with the sample for 1 hour at 25°C. An Alexa Fluor® 488 conjugated goat anti-rabbit antibody was used as the secondary.
プロトコール
参考文献
This product has been referenced in:
- Pignanelli C et al. Low-load resistance training to task failure with and without blood flow restriction: muscular functional and structural adaptations. Am J Physiol Regul Integr Comp Physiol 318:R284-R295 (2020). WB ; Human . Read more (PubMed: 31823670) »
- Cummins N et al. Disease-associated tau impairs mitophagy by inhibiting Parkin translocation to mitochondria. EMBO J 38:N/A (2019). Read more (PubMed: 30538104) »
