Anti-SOCS4 抗体 (ab3607)
Key features and details
- Goat polyclonal to SOCS4
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-SOCS4 antibody -
製品の詳細
Goat polyclonal to SOCS4 -
由来種
Goat -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Cow, Pig
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免疫原
Synthetic peptide:
SKVRVLRIDAPEQQ
, corresponding to C terminal amino acids 426-439 of Human SOCS7. -
特記事項
Principal Names – SOCS7; SOCS4; suppressor of cytokine signaling 7; SH2 domain containing SOCS box protein. Official Gene Symbol - SOCS7. GenBank Accession Number – NP_543143. LocusLink ID - 122809 (human). Gene Ontology terms - intracellular signaling cascade; regulation of cell growth.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab3607 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 1 - 3 µg/ml. 1 hour primary incubation is recommended for this product. |
|
| IHC-P | Use a concentration of 3 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. May be a substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Contains 1 SH2 domain.
Contains 1 SOCS box domain. -
ドメイン
The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes. - Information by UniProt
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参照データベース
- Entrez Gene: 540412 Cow
- Entrez Gene: 122809 Human
- Entrez Gene: 67296 Mouse
- Entrez Gene: 100302366 Pig
- Omim: 605118 Human
- SwissProt: Q0VC91 Cow
- SwissProt: Q8WXH5 Human
- SwissProt: Q91ZA6 Mouse
see all -
別名
- CIS4 antibody
- Cish4 antibody
- Cytokine inducible SH2 protein 4 antibody
see all
画像
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Western blot - Anti-SOCS4 antibody (ab3607)ab3607 at a 2
ab3607 at a 2µg/ml concentration staining ~50 kDa SOCS 7 in NIH-3T3 cell lysate (RIPA buffer, 30µg total protein per lane) by Western blot. 1 hour incubation, ECL detection.µ g/ml concentration staining ~50 kDa SOCS 7 in NIH-3T3 cell lysate (RIPA buffer, 30µ g total protein per lane) by Western blot. 1 hour incubation, ECL detection. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS4 antibody (ab3607)ab3607 (3.8µg/ml) staining of paraffin embedded Human Pancreas shows cytoplasm staining on select beta cells in islet of Langerhans. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
プロトコール
データシートおよび資料
参考文献 (0)
ab3607 は論文での使用が確認できていません。
