Anti-SOCS4 抗体 (ab3607)
Key features and details
- Goat polyclonal to SOCS4
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-SOCS4 antibody -
製品の詳細
Goat polyclonal to SOCS4 -
由来種
Goat -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Cow, Pig
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免疫原
Synthetic peptide:
SKVRVLRIDAPEQQ
, corresponding to C terminal amino acids 426-439 of Human SOCS7. -
特記事項
Principal Names – SOCS7; SOCS4; suppressor of cytokine signaling 7; SH2 domain containing SOCS box protein. Official Gene Symbol - SOCS7. GenBank Accession Number – NP_543143. LocusLink ID - 122809 (human). Gene Ontology terms - intracellular signaling cascade; regulation of cell growth.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab3607 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 1 - 3 µg/ml. 1 hour primary incubation is recommended for this product. |
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| IHC-P | Use a concentration of 3 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. May be a substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Contains 1 SH2 domain.
Contains 1 SOCS box domain. -
ドメイン
The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes. - Information by UniProt
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参照データベース
- Entrez Gene: 540412 Cow
- Entrez Gene: 122809 Human
- Entrez Gene: 67296 Mouse
- Entrez Gene: 100302366 Pig
- Omim: 605118 Human
- SwissProt: Q0VC91 Cow
- SwissProt: Q8WXH5 Human
- SwissProt: Q91ZA6 Mouse
see all -
別名
- CIS4 antibody
- Cish4 antibody
- Cytokine inducible SH2 protein 4 antibody
see all
画像
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Western blot - Anti-SOCS4 antibody (ab3607)ab3607 at a 2
ab3607 at a 2µg/ml concentration staining ~50 kDa SOCS 7 in NIH-3T3 cell lysate (RIPA buffer, 30µg total protein per lane) by Western blot. 1 hour incubation, ECL detection.µ g/ml concentration staining ~50 kDa SOCS 7 in NIH-3T3 cell lysate (RIPA buffer, 30µ g total protein per lane) by Western blot. 1 hour incubation, ECL detection. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS4 antibody (ab3607)ab3607 (3.8µg/ml) staining of paraffin embedded Human Pancreas shows cytoplasm staining on select beta cells in islet of Langerhans. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
プロトコール
データシートおよび資料
参考文献 (0)
ab3607 は論文での使用が確認できていません。
